Summary
An electron microscopic study aimed at differentiation between “castration gonadotrophs” and “posthepatectomy STH cells” was performed on the pars distalis of the pituitary of castrated male mice, after partial hepatectomy. The ultrastructural features observed permit the distinction of both cell types. In the present experiments some remarkable ultrastructural changes, other than those described in a previous report, have been found in STH cells of hepatectomized mice with or without previous castration. Most of them contained masses of heterogeneous electron density, suggesting fusion of granules. These masses and some secretion granules were observed close to the plasma membrane, apparently in the process of discharging material into the pericapillary space. Granular extrusion was more frequent than normally. An increased number of lysosomes, probably related to the digestion of overproduced secretion material, was evident. The appearance of concentric lamellar formations might be related to an increase in cell movements. STH cells with severe cytoplasmic damage were also found, indicating an increased rate of cell loss.
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Badrán, A.F., Echave Llanos, J.M., Surur, J.M.: The pars distalis of the pituitary as a source of liver growth stimulating factors during liver regeneration. Virchows Arch. Abt. B. 13, 79–85, (1973)
Badrán, A.F., Nash, R.E., Echave Llanos, J.M.: Ultrastructure of STH cells of the pars distalis of the hypophysis of mice bearing transplanted hepatomas. Z. Zellforsch. 118, 525–531 (1971)
Behrens, U., Martin, C.: Ultrastructural changes in the pars distalis of the maturing male rat. J. Morph. 136, 53–78 (1972)
Brues, A.M., Drury, D.R., Brues, M.C.: A quantitative study of cell growth in regenerating liver. Arch. Path. 22, 658–673 (1936)
Clementi, F., De Virgiliis, G.: Ultrastructure de l'adénohypophyse après ovariectomie et traitement par les oestrogènes et la progesterone. Path. et Biol. 15, 119–131 (1967)
Coates, P.W., Ashby, E.A., Krulich, L., Dhariwal, A.P.S., McCann, S.M.: Morphologic alterations in somatotrophs of the rat adenohypophysis following administration of hypothalamic extracts. Amer. J. Anat. 128, 389–412 (1970)
De Duve, Ch., Wattiaux, R.: Function of lysosomes. Ann. Rev. Physiol. 28, 435–492 (1966)
De Virgiliis, G., Meldolesi, J., Clementi, F.: Ultrastructure of growth hormone producing cells of rat pituitary after injection of hypothalamic extract. Endocrinology 83, 1278–1284 (1968)
Dubois, P., Girod, Ch.: Formations lamellaires concentriques dans des cellules antéhypophysaires chez le hamster doré. Z. Zellforsch. 115, 196–211 (1971)
Echave Llanos, J.M., Gómez Dumm, C.L.A.: Release of growth hormone from STH cells of hepatectomized mice without the participation of the Golgi complex. Experientia (Basel) 27, 318 (1971b)
Echave Llanos, J.M., Gómez Dumm, C.L.A., Nessi, A.C.: Ultrastructure of STH cells of the pars distalis of hepatectomized mice. Z. Zellforsch. 113, 29–38 (1971a)
Echave Llanos, J.M., Gómez Dumm, C.L.A., Surur, J.M.: Growth hormone release after hepatectomy. Experientia (Basel) 27, 574 (1971c)
Farquhar, M.G., Rinehart, J.F.: Electron microscopic studies of the anterior pituitary gland of castrated rats. Endocrinology, 54, 516–540 (1954)
Gómez Dumm, C.L.A., Echave Llanos, J.M.: Further studies on the ultrastructure of the pars distalis of the male mouse hypophysis. Acta anat. (Basel) 82, 254–266 (1972)
Luft, J.: Improvements in epoxy resin embedding methods. J. biophys. biochem. Cytol. 9, 409–414 (1961)
Millonig, G.: Further observations on a phosphate buffer for osmium solutions in fixation. In: S.S. Breeze (ed.), Fifth International Congress for Electron Microscopy vol. 2, 8. New York-London: Academic Press 1962
Reynolds, E.S.: Use of lead citrate at high pH as an electron opaque stain in electron microscopy. J. Cell Biol. 17, 208–212 (1963)
Smith, R.E., Farquhar, M.G.: Lysosome function in the regulation of the secretory process in cells of the anterior pituitary gland. J. Cell Biol. 31, 319–347 (1966)
Yamada, K., Nakamura, M., Yamashita, K.: Light and electron microscopic studies on the adenohypophysis of a diabetic (KK) strain of the mouse. Z. Zellforsch. 79, 429–442 (1967)
Zotter, S.: Elektronenmikroskopische Befunde an den STH-Zellen der Mäusehypophyse nach partieller Hepatektomie. Exp. Path. 6, 130–140 (1972)
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Work carried out with the financial assistance by a grant from the Comisión de Investigación Científica de la Universidad Nacional de La Plata. Thanks are due to the members of the Technical Staff of the Institute for their technical assistance.
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Echave Llanos, J.M., Gómez Dumm, C.L.A. Ultrastructure of STH cells of the pars distalis of castrated male mice after hepatectomy. Z.Zellforsch 144, 31–35 (1973). https://doi.org/10.1007/BF00306684
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DOI: https://doi.org/10.1007/BF00306684