Summary
Falck's method for the demonstration of monoamines was applied to the flatworm Schistosoma mansoni. Whole mount preparations and sections of frozen dried specimens showed that a primary catecholamine is present in four pairs of large nerve cells and in two longitudinal fiber tracts showing varicosities. Smaller neurons were found along these tracts. They are united by commissures and give off many branches which end in small dilatations.
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Angelakos, E. T., King, M. P.: Demonstration of nerve terminals, containing adrenaline by a new histochemical technique. Nature (Lond.) 213, 391–392 (1967).
Bueding, E., Schiller, E. L., Burgeois, J. C.: Some physiological biochemical and morphologic effects of tris (p-aminophenil) carbonium salts (TAC) on Schistosoma mansoni. Amer. J. trop. Med. Hyg. 16, 500–515 (1967).
Bullock, T. H., Horridge, G. A.: Structure and function in the nervous system of invertebrates, vol. 1. San Francisco: W. A. Freeman & Co. 1965.
Corrodi, H., Jonsson, G.: The formaldehyde fluorescence method for the histochemical demonstration of biogenic monoamines. A review of the methodology. J. Histochem. Cytochem. 15, 65–78 (1967).
Cottrell, G. A., Laverak, M. S.: Invertebrate pharmacology. Ann. Rev. Pharmacol. 8, 273–298 (1968).
Dahl, E., Falck, B., Mecklenburg, C. von, Myhrberg, H.: Adrenergic sensory neurons in invertebrates. Gen. comp. Endocrinol. 3, 693 (1963).
Eränkö, O.: Distribution of fluorescing islet, adrenaline and noradrenaline in the adrenal medulla of the hamster. Acta endocr. (Kbh.) 18, 174–179 (1955).
Falck, B., Owman, Ch.: A detailed methodological description of the fluorescence method for the cellular demonstration of biogenic monoamines. Acta Univ. Lund., Sect. II, 7, 1–23 (1965).
Fripp, P. J.: Histochemical localization of esterase activity in Schistosomes. Exp. Parasit. 21, 380–390 (1967).
Plotnikova, S. I., Kuzmina, L. V.: In: Sakharov, D. A., 1970.
Sakharov, D. A.: Cellular aspects of invertebrate neuropharmacology. Ann. Rev. Pharmacol. 10, 335–352 (1970).
Scharrer, B.: Neurohumors and neurohormones: definitions and terminology. J. Neurovisc. Rel., Suppl. 9, 1–20 (1969).
Silk, M. H., Spence, I. M.: Ultrastructural studies on the blood fluke Schistosoma mansoni III. The nerve tissue and sensory structures. S. Afr. J. med. Sci. 34, 93–104 (1969).
Tranzer, J. P., Thoenen, H., Snipes, R. L., Richards, J. G.: Recent developments on the ultrastructural aspect of adrenergic nerve endings in various experimental conditions. In: Mechanisms of synaptic transmission (eds. K. Akert and P. G. Waser). Progress in Brain Research 31, 33–46 (1969).
Welsh, J. H., Williams, L. D.: Monoamines containing neurons in planaria. J. comp. Neurol. 138, 103–116 (1970).
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Contribution number 14 from the Schistosomiasis Research Unit, Institute of Biological Sciences, Federal University of Minas Gerais, Brazil. This study has been supported by research grants from the U.S. Army (DAAC 19-70-G-0023 and DAHG-70-G-0028), CAPES and Conselho Nacional de Pesquisas, Brazil. For technical assistance we thank Mr. Rubens Miranda, technician supported by the “Conselho Nacional de Pesquisas” of Brazil.
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Machado, C.R.S., Machado, A.B.M. & Pellegrino, J. Catecholamine-containing neurons in Schistosoma mansoni . Z.Zellforsch. 124, 230–237 (1972). https://doi.org/10.1007/BF00335681
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DOI: https://doi.org/10.1007/BF00335681