Abstract
Molecular cloning studies have elucidated the presence of multiple isoforms of mammalian adenylyl cyclase. So far, six different isoforms (I to VI) have been fully characterized. Comparison of their structural and biochemical characteristics suggests that the mammalian adenylyl cyclase family can be classified into four sub-families: type I, type III, type II/IV, and type V/VI. We have determined the chromosomal localization of these genes. Type I gene was assigned to chromosome 7, type III to chromosome 2, types II and IV to chromosomes 5 and 14, and types V and VI to chromosomes 3 and 12. Our results indicate that the different adenylyl cyclase isoforms, even within the same subfamily, are distributed randomly in the genome, in contrast to the chromosomal organization of other components within the same signaling pathway, such as catecholamine receptors and G proteins.
Similar content being viewed by others
References
Blatt C, Eversole-Cire P, Cohn VH, Zollman S, Fournier REK, Mohandas LT, Nesbitt M, Lugo T, Jones DT, Reed RR, Weiner LP, Sparkes RS, Simon MI (1988) Chromosomal localization of genes encoding guanine nucleotide-binding protein subunits in mouse and human. Proc Natl Acad Sci USA 85:7642–7646
Bloch DB, Bloch KD, Iannuzzi M, Collins FS, Neer EJ, Seidman JG, Morton CC (1988) The gene for the alpha-i-1 subunit of human guanine nucleotide binding protein maps near the cystic fibrosis locus. Am J Hum Genet 42:884–888
Katsushika S, Chen L, Kawabe J, Nilakantan R, Halnon NJ, Homey CJ, Ishikawa Y (1992) Cloning and characterization of a sixth adenylyl cyclase isoforms: types V and VI constitute a subgroup within the mammalian adenylyl cyclase family. Proc Natl Acad Sci USA 89:8774–8778
Levine MA, Modi WS, O'Brien SJ (1990) Chromosomal localization of the genes encoding two forms of the G protein beta polypeptide, beta 1 and beta 3, in man. Genomics 8:380–386
Magovcevic I, Ang SL, Seidman JG, Tolman CJ, Neer EJ, Morton CC (1992) Regional localization of the human G protein alpha i2 (GNAI2) gene: assignment to 3p21 and a related sequence (GNAI2L) to 12pl2-pl3. Genomics 12:125–129
Murtagh JJ, Eddy R, Shows TB, Moss J, Vaughan M (1991) Different forms of Go alpha mRNA arise by alternative splicing of transcripts from a single gene on human chromosome 16. Mol Cell Biol 11:1146–1155
Ruano G (1993) BIOS map. Somatic cell hybrid manual: method for human chromosomal localization. BIOS Laboratories, New Haven, pp 21–26
Stengel D, Parma J, Gannagé MH, Roeckel N, Mattei MG, Barouki R, Hanoune J (1992) Different chromosomal localization of two adenylyl cyclase genes expressed in human brain. Hum Genet 90:126–130
Tang WJ, Gilman AG (1993) Adenylyl cyclase. Cell 70:869–872
Wilkie TM, Gilbert DJ, Olsen AS, Chen XN, Amatruda TT, Kronenberg JR, Trask BJ, Jong PD, Reed RR, Simon MI, Jenkins NA, Copeland NG (1992) Evolution of the mammalian G protein alpha subunit multigene family. Nature Genet 1:85–91
Yang-Feng TL, Xue F, Zhong W, Cotecchia S, Frielle T, Caron MG, Lefkowitz RJ, Francke U (1990) Chromosomal organization of adrenergic receptor genes. Proc Natl Acad Sci USA 87:1516–1520
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Gaudin, C., Homey, C.J. & Ishikawa, Y. Mammalian adenylyl cyclase family members are randomly located on different chromosomes. Hum Genet 94, 527–529 (1994). https://doi.org/10.1007/BF00211020
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00211020