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Chromosomal damage in sperm of patients surviving Hodgkin's disease following MOPP (nitrogen mustard, vincristine, procarbazine, and prednisone) therapy with and without radiotherapy

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Abstract

Following fusion with hamster eggs, human sperm chromosomes from six Hodgkin's disease patients were analyzed to determine the genotoxic effects of therapy. Each patient had received two to six cycles of MOPP (nitrogen mustard, vincristine, procarbazine, and prednisone), with or without radiotherapy, from 3 to 20 years before the study. A total of 571 cells from the six patients were analyzed; 9.8% of the cells had structural aberrations, and 1.6% were hyperhaploid. Analysis of 5998 metaphases from a control group of 24 male donors revealed only 6.9% of cells with structural aberrations and 0.8% aneuploidy. The increase in hyperhaploidy in the patients was statistically significant. Thus, results of this study suggest that the MOPP regimen, with or without radiotherapy, is capable of causing chromosome abnormalities in the sperm of Hodgkin's disease patients.

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Authors and Affiliations

  1. Biology and Biotechnology Research Program, Lawrence Livermore National Laboratory, 94550, Livermore, CA, USA

    B. F. Brandriff, L. A. Gordon & A. V. Carrano

  2. Department of Experimental Radiotherapy, University of Texas M.D. Anderson Cancer Center, 77030, Houston, TX, USA

    M. L. Meistrich

  3. Department of Laboratory Medicine, University of Texas M.D. Anderson Cancer Center, 77030, Houston, TX, USA

    J. C. Liang

Authors
  1. B. F. Brandriff
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  2. M. L. Meistrich
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  3. L. A. Gordon
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  4. A. V. Carrano
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  5. J. C. Liang
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Brandriff, B.F., Meistrich, M.L., Gordon, L.A. et al. Chromosomal damage in sperm of patients surviving Hodgkin's disease following MOPP (nitrogen mustard, vincristine, procarbazine, and prednisone) therapy with and without radiotherapy. Hum Genet 93, 295–299 (1994). https://doi.org/10.1007/BF00212026

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  • DOI: https://doi.org/10.1007/BF00212026

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