Abstract
Familial multiple endocrine neoplasia type 2A (MEN 2A) is a cancer syndrome that is inherited as an autosomal dominant with high penetrance. Its clinical features are medullary carcinoma of the thyroid, pheochromocytomas, and hyperparathyroidism. A new polymorphic locus D10S97 (probe: KW6ΔSacI) detects a codominant EcoRI polymorphism that is tightly linked to the MEN2A locus. The peak lod score for linkage between D10S97 with MEN2A is 13.03 at θ=0.00. The polymorphic locus D10S97 maps, by linkage analysis, into the previously defined interval between FNRB and RBP3 to which MEN2A has been assigned. We present physical mapping data showing that the probe pKW6 originates from 10p13 and that the polymorphic locus D10S97 in 10q11.2 is detected by cross-hybridization.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Albertsen HM, Abderrahim H, Cann HM, Dausset J, Le Paslier D, Cohen D (1990) Construction and characterization of a yeast artificial chromosome library containing seven haploid genome equivalents. Proc Natl Acad Sci USA 87:4256–4260
Astrin KH, Warner CA, Yoo H-W, Goodfellow PJ, Tsai T-S, Desnick RJ (1991) Regional assignment of the human uroporphyrinogen III synthase (UROS) gene to chromosome 10q25.3–q26.3. Hum Genet 87:18–22
Botstein D, White RL, Skolnick M, Davis RW (1980) Construction of a genetic linkage map in man using restriction fragment length polymorphisms. Am J Hum Genet 32:314–331
Bowcock AM, Bucci C, Hebert JM, Kidd JR, Kidd KK, Friedlaender JS, Cavalli-Sforza LL (1987) Study of 47 DNA markers in five populations from four continents. Gene Geogr 1:47–64
Brooks-Wilson AR, Povey D, Nevanlinna HA, De Jong PJ, Goodfellow PJ (1990) Rapid cloning and characterization of new chromosome 10 DNA markers by ALU element-mediated PCR. Genomics 7:614–620
Carson NL, Simpson NE (1991) A physical map of human chromosome 10 and a comparison with an existing genetic map. Genomics 11:379–388
Carson NL, Wu J, Jackson CE, Kidd KK, Simpson NE (1990) The mutation for medullary thyroid carcinoma with parathyroid tumors (MTC with PTs) is closely linked to the centromeric region of chromosome 10. Am J Hum Genet 47:946–951
Gagel RF, Tashjian AH, Cummings T. et al (1988) The clinical outcome of prospective screening for multiple endocrine neoplasia type 2A: an 18-year experience. N Engl J Med 318:478–484
Green ED, Olson MV (1990) Systematic screening of yeast artificial-chromosome libraries by use of the polymerase chain reaction. Proc Natl Acad Sci USA 87:1213–1217
Goodfellow PJ, Myers S, Anderson LL, Brooks-Wilson AR, Simpson NE (1990) a new marker (D10S94) very tightly linked to the multiple endocrine neoplasia type 2A (MEN2A) locus. Am J Hum Genet 47:952–956
Kidd KK, Simpson NE (1990) The search for the gene for MEN 2A. Rec Adv Horm Res 46:305–343
Kidd JR, Black FL, Weiss KM, Balazs I, Kidd KK (1991) Studies of Amerindian populations using nuclear DNA polymorphisms. Hum Biol 63:775–794
Lichter P, Tang C-JC, Call K, Hermanson G, Evans G, Housman D, Ward DC (1990) High resolution mapping of human chromosome 11 by in situ hybridization with cosmid clones. Science 247:64–69
Lichter JB, Wu J, Genel M, Flynn SD, Pakstis AJ, Kidd JR, Kidd KK (1992a) Pre-symptomatic testing using DNA markers for individuals at-risk for familial MEN 2A. J Clin Endocrinol Metab 74:368–373
Lichter JB, Wu J, Miller D, Goodfellow PJ, Kidd KK (1992b) A high resolution meiotic mapping panel for the pericentromeric region of chromosome 10. Genomics 13:607–612
Mathew CGP, Chin KS, Easton DF, Thorpe K, Carter C, Liou GI, Fong S-L, Bridges CDB, Haak H, Nieuwcnhuijzen Druseman AC, Schifter S, Hansen HH, Telenius H, Telenius-Berg M, Ponder BAJ (1987) A linked genetic marker for multiple endocrine neoplasia type 2A on chromosome 10. Nature 328:527–528
Mathew CGP, Wakeling W, Jones E, Easton D, Fisher R, Strong C, Smith C, Chin K, Little P, Nakamura Y, Shows TB, Jones C, Goodfellow PJ, Povey S, Ponder BAJ (1990) Regional localization of polymorphic markers on chromosome 10 by physical and genetic mapping. Ann Hum Genet 54:121–129
Mathew CGP, Easton DF, Nakamura Y, Ponder BAJ, and the International MEN 2A community (1991) Presymptomatic screening for multiple endocrine neoplasia type 2A with linked DNA markers. Lancet 337:7–11
Ott J (1985) Analysis of human genetic linkage. Johns Hopkins University Press, Baltimore
Simpson NE, Cann HM (1992) Report of the Committee on the Genetic Constitution of Chromosome 10. Cytogenet Cell Genet 58:428–458
Simpson NE, Kidd KK, Goodfellow PJ, McDermid H, Myers S, Kidd JR, Jackson CE, Duncan AMV, Farrer LA, Brasch K, Castiglione C, Genel M, Gertner J, Greenberg CR, Gusella JF, Holden JJA, White BN (1987) Assignment of multiple endocrine neoplasia type 2A to chromosome 10 by linkage. Nature 328:528–530
Wu J, Kidd KK (1990a) Extensive sequence polymorphisms associated with chromosome 10 alpha satellite DNA and its close linkage to markers from the pericentromeric region. Hum Genet 84:279–282
Wu J, Kidd KK (1990b) An EcoRI polymorphism identified by KW6 (D10S97) on chromosome 10. Nucleic Acids Res 18:1316
Wu J, Carson N, Myers S, Pakstis A, Kidd J, Castiglione C, Anderson L, Hoyle L, Genel M, Simpson N, Kidd KK (1990) The genetic defect in multiple endocrine neoplasia type 2A maps next to the centromere of chromosome 10. Am J Hum Genet 46:624–630
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Lichter, J.B., Wu, J., Brooks-Wilson, A.R. et al. A new polymorphic marker (D10S97) tightly linked to the multiple endocrine neoplasia type 2A (MEN2A) locus. Hum Genet 90, 516–520 (1993). https://doi.org/10.1007/BF00217451
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00217451