Summary
Linkage analysis of four polymorphic anonymous DNA markers from the Xp22 region was performed using families from the Centre d'Etude du Polymorphisme Humain. The loci DXS43 (pD2) and DXS16 (pXUT23) were found to be tightly linked (\(\hat \theta \)= 0.02 at\(\hat Z\)= 14.96) and proximal to both DXS85 (782) and DXS143 (dic56). Multipoint linkage analysis suggests the order:
References
Aldridge J, Kunkel L, Bruns G, Tantravahi U, LaLande M, Brewster T, Moreau E, Wilson M, Bromley W, Roderick T, Latt SA (1984) A strategy to reveal high frequency RFLPs along the human X chromosome. Am J Hum Genet 36:546–564
Alitalo T, Karna J, Forsius H, Chapelle A de la (1987) X-linked retinoschisis is closely linked to DXS41 and DXS16 but not DXS85. Clin Genet 32:192–195
Arveiler B, Oberlé I, Mandel JL (1987) Genetic mapping of nine DNA markers in the q11-22 region of the human X chromosome. Genomics 1:60–66
Bakker E, Goor N, Wrogemann K, Kunkel LM, Fenton WA, Majoor-Krakauer D, Jahoda MGJ, Van Ommen GJB, Hofker MH, Mandel JL, Davies KE, Willard HF, Sandkuyl L, Essen AJV, Sachs ES, Pearson PL (1985) Prenatal diagnosis and carrier detection of Duchenne muscular dystrophy with closely linked RFLPs. Lancet I:655–658
Brown CJ, Willard HF (1987) MspI RFLP detected with chromosome-walk clone pXUT23-SE3.2L from DXS16 in Xp22.1-22.3. Nucleic Acids Res 15:9614
Conneally PM, Edwards JH, Kidd KK, Lalouel J-M, Morton NE, Ott J, White R (1985) Report of the committee on methods of linkage analysis and reporting. Cytogenet Cell Genet 40:356–359
Dahl N, Goonewardena P, Chotai J, Anvret M, Pettersson U (1988) DNA linkage analysis of X-linked retinoschisis. Hum Genet 78: 228–232
Davies KE, Kenwrick S, Forrest S, Ball SP, Ingle C, Bates G, Ellis KM, Mandel JM, Kunkel LM, Willard H, Williamson R, Pearson PL, Benham FJ (1985) Physical mapping of RFLP's surrounding the Duchenne muscular dystrophy locus. Cytogenet Cell Genet 40:613
Davies KE, Mandel J-L, Weissenbach J, Fellous M (1988) Report of the committee on the genetic constitution of the X and Y chromosomes. Cytogenet Cell Genet 46:277–315
Donis-Keller H, Green P, Helms C, Cartinhour S, Weiffenback B, Stephens K, Keith TP, Bowden DW, Smith DR, Lander ES, Botstein D, Akots G, Redider KS, Gravius T, Brown VA, Rising MB, Parker C, Watt JA, Kauffman DE, Bricker ER, Phipps P, Muller-Kahle H, Fulton TR, Ng S, Schumm JW, Braman JC, Knowlton RG, Barker DF, Crooks SM, Lincoln SE, Daly SE, Abrahamson J (1987) A genetic linkage map of the human genome. Cell 51:319–337
Drayna D, White R (1985) The genetic linkage map of the human X chromosome. Science 230:753–758
Feinberg AP, Vogelstein B (1983) A technique for radiolabeling DNA fragments to high specific activity. Anal Biochem 132:6–13
Hofker MH, Wapenaar MC, Goor N, Bakker E, Van Ommen GJB, Pearson PL (1985) Isolation of probes detecting restriction fragment length polymorphism from X chromosome-specific libraries: potential use for diagnosis of Duchenne muscular dystrophy. Hum Genet 70:148–156
Kidd JR, Castiglione CM, Davies KE, Pakstis AJ, Gusella J, Sparkes RS, Pearson P, Willard H, Kidd KK (1985) Mapping the locus for X-linked ocular albinism (OA). Am J Hum Genet 37:A161
Lathrop GM, Lalouel JM, White RL (1986) Construction of human linkage maps: likelihood calculations for multilocus linkage analysis. Genet Epidemiol 3:39–52
Mahtani MM, Willard HF (1988) A primary genetic map of the pericentromeric region of the human X chromosome. Genomics 2: 294–301
Middlesworth W, Bertelson C, Kunkel LM (1985) An RFLP detecting single copy X-chromosome fragment, dic56, from Xp22-Xpter [HGM8 assignment no. DXS143]. Nucleic Acids Res 13: 5723
Ott J (1974) Estimation of the recombination fraction in human pedigrees: efficient computation of the likelihood for human linkage studies. Am J Hum Genet 8:80–96
Thakker RV, Read AP, Davies KE, Whyte MP, Weksberg R, Glorieux F, Davies M, Mountford RC, Harris R, King A, Kim GS, Fraser D, Kooh SW, O'Riordan JLH (1987) Bridging markers defining the map position of X linked hypophosphataemic rickets. J Med Genet 24:756–760
Willard HF, Smith KD, Sutherland J (1983) Isolation and characterization of a major tandem repeat family from the human X chromosome. Nucleic Acids Res 11:2017–2033
Yates JRW, Goudie DR, Gillard EF, Aitken DA, Affara NA, Clayton JF, Tippett PA, Ferguson-Smith MA (1987) Multipoint linkage analysis of steroid sulfatase (X-linked ichthyosis) and distal Xp markers. Genomics 1:52–59
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Brown, C.J., Mahtani, M.M. & Willard, H.F. Genetic mapping of four DNA markers (DXS16, DXS43, DXS85, and DXS143) from the p22 region of the human X chromosome. Hum Genet 80, 296–298 (1988). https://doi.org/10.1007/BF01790101
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DOI: https://doi.org/10.1007/BF01790101