Summary
Recently, using an APOE cDNA probe, we discovered an Hpa I restriction fragment length polymorphism (RFLP) that appeared to be strongly associated with the expression of type III hyperlipoproteinemia (Klasen et al. 1987). In the present report it is shown that the same Hpa I RFLP can be revealed with both the APOC1 and APOC2 cDNA probes. This enabled us to localize the polymorphic Hpa I site between the APOE and APOC1 genes and to localize the APOC2 gene approximately 22 kb 3′ of the APOC1 pseudogene on chromosome 19.
This is a preview of subscription content, log in via an institution to check access.
Reference
Boguski MS, Birkenmeier EH, Elshourbagy NA, Taylor JM, Gordon JI (1986) Evolution of the apolipoproteins: structure of the rat apo AIV gene and its relationship to the human genes for apo AI, CIII and E. J Biol Chem 261:6398–6407
Bruns GAP, Karathanasis SK, Breslow JL (1984) Human apolipoprotein AI-CIII gene complex is located on chromosome 11. Arteriosclerosis 4:97–102
Das HK, McPherson J, Bruns GAP, Karathanasis SK, Breslow JL (1985) Isolation, characterization and mapping to chromosome 19 of the human apolipoprotein E gene. J Biol Chem 260:6240–6247
Davison PJ, Norton P, Wallis SC, Gill L, Cook M, Williamson R, Humphries SE (1986) There are two gene sequences for human apolipoprotein C1 (apo C1) on chromosome 19, one of which is 4kb from the gene for apo E. Biochem Biophys Res Commun 136:876–884
Donald JA, Wallis SC, Kessling A, Tippett P, Robson EB, Ball S, Davies KE, Scambler P, Berg K, Heiberg A, Williamson R, Humphries SE (1985) Linkage relationships of the gene for apolipoprotein CII with loci on chromosome 19. Hum Genet 69:39–43
Ferns GAA, Galton DJ (1986) Haplotypes of the human apoprotein AI-CIII-AIV gene cluster in coronary atherosclerosis. Hum Genet 73:245–249
Hofker MH, Wapenaar MC, Goor N, Bakker E, Ommen G-JB van, Pearson PL (1985) Isolation of probes detecting restriction fragment length polymorphisms from X chromosome-specific libraries: potential use for diagnosis of Duchenne muscular dystrophy. Hum Genet 70:148–156
Humphries SE, Berg K, Gill L, Cumming AM, Robertson FW, Stalenhoef AFH, Williamson R, Borresen AL (1984) The gene for apolipoprotein CII is closely linked to the gene for apolipoprotein E on chromosome 19. Clin Genet 26:389–396
Karathanasis SK (1985) Apolipoprotein multigene family: tandem organization of human apolipoprotein AI, CIII and AIV genes. Proc Natl Acad Sci USA 82:6374–6378
Karathanasis SK, Yunis I, Zannis VI (1986) Structure, evolution and tissue-specific synthesis of human apolipoprotein AIV. Biochemistry 25:3962–3970
Klasen EC, Talmud PJ, Havekes L, Wit E de, Kooij-Meijs E van der, Smit M, Hansson G, Humphries SE (1987) A common restriction fragment length polymorphism of the human apolipoprotein E gene and its relationship to type III hyperlipidaemia. Hum Genet 75:244–247
Knott TJ, Robertson LM, Priestley LM, Urdea M, Wallis S, Scott J (1984) Characterisation of mRNAs encoding the precursor for human apolipoprotein CI. Nucleic Acids Res 12:3909–3915
Luo C-C, Li W-H, Moore MN, Chan L (1986) Structure and evolution of the apolipoprotein multigene family. J Mol Biol 187:325–340
Myklebost O, Ronge S (1986) The gene for human apolipoprotein CI is located 4.3 kilobases away from the apolipoprotein E gene on chromosome 19. Hum Genet 73:286–289
Myklebost O, Rogne S, Olaisen B, Gedde-Dahl T Jr, Prydz H (1984a) The locus for apolipoprotein CII is closely linked to the apolipoprotein E locus on chromosome 19 in man. Hum Genet 67:309–312
Myklebost O, Williamson B, Markham AF, Myklebost SR, Rogers J, Woods DE, Humphries SE (1984b) The isolation and characterization of cDNA clones for human apolipoprotein CII. J Biol Chem 259:4401–4404
Olaisen B, Teisberg P, Gedde-Dahl T Jr (1982) The locus for apolipoprotein E (apo E) is linked to the complement component C3 (C3) locus in chromosome 19 in man. Hum Genet 62:233–236
Ommen G-JB van, Arnberg AC, Bass F, Brocas H, Sterk A, Tegelaers WHH, Vassart G, Vijlder JJM de (1983) The human thyroglobulin gene contains two 15–17 kb introns near its 3′ end. Nucleic Acids Res 11:2273–2285
Scott J, Knott TJ, Shaw DJ, Brook JD (1985) Localization of genes encoding apolipoproteins CI, CII and E to the p13→cen region of human chromosome 19. Hum Genet 71:144–146
Tata F, Henry I, Markham AF, Wallis SC, Weil D, Grzeschik KH, Junien C, Williamson R, Humphries SE (1985) Isolation and characterization of a cDNA clone for human apolipoprotein C1 and assignment of the gene to chromosome 19. Hum Genet 69: 345–349
Zannis VI, McPherson J, Goldberger G, Karathanasis SK, Breslow JL (1984) Synthesis, intracellular processing and signal peptide of human apolipoprotein E. J Biol Chem 259:5495–5499
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Smit, M., van der Kooij-Meijs, E., Frants, R.R. et al. Apolipoprotein gene cluster on chromosome 19. Hum Genet 78, 90–93 (1988). https://doi.org/10.1007/BF00291243
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00291243