Summary
Leukemic cells with reciprocal translocations involving 11p13 and 14q13 were obtained from two patients with T-cell acute lymphoblastic leukemia and fused with mouse Ltk- cells. DNA from independent hybrid clones was screened by Southern blot and hybridization to molecular probes for the human catalase and Ha-ras-1 genes. Several clones showed segregation of these two genes, indicating the presence of either the der 11 or der 14 human chromosomes. When DNA from these hybrid clones was examined for the presence of the human genes for calcitonin and γ-globin, both genes were found to segregate with the Ha-ras-1 gene and the der14 chromosome indicating that they lie distal to catalase. When the hybrid clones were examined for the presence of human lactate dehydrogenase A (LDH A) activity, only those clones containing the der14 chromosome expressed activity indicating that the LDH A gene is also distal to catalase on the short arm of chromosome 11.
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Botstein D, White RL, Skolnick M, Davis RW (1980) Construction of a genetic linkage map in man using restriction fragment length polymorphisms. Am J Genet 32:314–331
Bruns G, Kumit D, Gerhard DS, Housman D (1984) cDNA clones on chromosome 11p and 12p identified with LDH oligonucleotides. Cytogenet Cell Genet 37:428
Chaganti RSK, Jhanwar SC, Antonarakis SE, Hayward WS (1985) Germ-line chromosomal localization of genes in chromosome 11p linkage: Parathyroid hormone, β-globin, c:Ha-ras-1, and insulin. Somatic Cell Mol Genet 11:197–202
De Martinville B, Francke U (1983) The c-Ha-ras-1, insulin, and β-globin map outside the delection associated with aniridia-Wilms' tumour. Nature 305:641–643
De Martinville B, Giacalone J, Shih C, Weinberg RA, Francke U (1983) Oncogene from human EJ bladder carcinoma is located on the short arm of chromosome 11. Science 219:498–501
Francke U, Oliver N (1978) Quantitative analysis of high-resolution trypsin-Giemsa bands on human prometaphase chromosomes. Hum Genet 45:137–165
Francke U, George DL Brown MG, Riccardi VM (1977) Gene dosage effect: intraband mapping of the LDH A locus using cells from four individuals with different interstitial deletions of 11p. Cytogenet Cell Genet 19:197–207
Gerald PS, Grzeschik KH (1984) Report of the committee on the genetic constitution of chromosomes 10, 11, and 12. Human Gene Mapping 7. Cytogenet Cell Genet 37:103–126
Gusella J, Varsanyi-Breiner A, Kao F-T, Jones C, Puck TT, Keys C, Orkin S, Housman D (1979) Precise localization of human β-globin gene complex on chromosome 11. Proc Natl Acad Sci USA 76:5239–5243
Höppener JW, Steenbergh PH, Zandberg J, Bakker E, Pearson PL, Geurts van Kessel AHM, Jansz HS, Lips CJM (1984) Localization of the polymorphic human calcitonin gene on chromosome 11. Hum Genet 66:309–312
Huerre C, Despoisse S, Gilgenkrantz S, Lenoir GM, Junien C (1983) c-Ha-ras-1 is not deleted in aniridia-Wilms' tumour association. Nature 305:638–641
Jhanwar SC, Neel BG, Hayward WS, Chaganti RSK (1980) Localization of c-ras oncogene family on human germ-line chromosomes. Proc Natl Acad Sci USA 80:4794–4797
Junien C, Turleau C, De Grouchy J, Said R, Rethore M-O, Tenconi R, Dufier JL (1980) Regional assignment of catalase (cat) gene to band 11p13. Association with the aniridia-Wilms' tumor-gonadoblastoma (WAGR) complex. Ann Genet (Paris) 23:165–168
Korneluk RG, Quan F, Lewis WH, Guise KS, Willard HF, Holmes MT, Gravel RA (1984) Isolation of human fibroblast catalase cDNA clones. J Biol Chem 259:13819–13823
Lebo RV, Carrano AV, Burkhart-Schultz KJ, Dozy AM, Yu L-C, Kan YW (1979) Assignment of human β-, γ- and δ-globin genes to the short arm of chromosome 11 by chromosome sorting and DNA restriction enzyme analysis. Proc Natl Acad Sci USA 76:5804–5808
Lebo RV, Cheung M-C, Bruce BD, Riccardi VM, Kao F-T, Kan YW (1985) Mapping parathyroid hormone, β-globin, insulin, and LDH-A genes within the human chromosome 11 short arm by spot blotting sorted chromosomes. Hum Genet 69:316–320
Lewis WH, Michalopoulos EE, Williams DL, Minden MD, Mak TW (1985) Breakpoints in the human T-cell antigen receptor α-chain locus in two T-cell leukaemia patients with chromosomal translocations. Nature (in press)
Malcolm S, Barton P, Murphy C, Ferguson-Smith MA (1981) Chromosomal localization of a single gene by in situ hybridization-human β-globin genes on the short arm of chromosome 11. Ann Hum Genet 45:135–141
Meera Khan P (1971) Enzyme electrophoresis on cellulose acetate gel: zymogram patterns in man-mouse and man-Chinese hamster somatic cell hybrids. Arch Biochem Biophys 145:470–483
Michalopoulos EE, Bevilacqua PJ, Stokoe N, Powers VE, Willard HF, Lewis WH (1985) Molecular analysis of gene deletion in aniridia-Wilms tumor association. Hum Genet 70:157–162
Morton CC, Kirsch IR, Taub R, Orkin SH, Brown JA (1984) Localization of the β-globin gene by chromosomal in situ hybridization. Am J Hum Genet 36:576–585
Narahara K, Kikkawa K, Kimoto H, Ogata M, Kasai R, Hamawaki M, Matsuoka K (1984) Regional mapping of catalase and Wilms tumor-aniridia, genitourinary abnormalities, and mental retardation triad loci to the chromosome segment 11p1305-p1306. Hum Genet 66:181–185
Oi VT, Herzenberg LA (1980) Immunoglobulin-producing hybrid cell lines. In: Mishell B, Shiigi SM (eds) Selected methods in cellular immunology. Freeman, San Francisco, pp 351–372
Quan F, Korneluk RG, Gravel RA (1985) Isolation and characterization of the gene for human catalase. Am J Hum Genet (in press)
Simola KOJ, Knuutila S, Kaitila I, Pirkola A, Pohja P (1983) Familial aniridia and translocation t(4;11)(q22;p13) without Wilms tumor. Hum Genet 63:158–161
Wieacker P, Mueller CR, Mayerova A, Grzeschik KH, Ropers HH (1980) Assignment of the gene coding for human catalase to the short arm of chromosome 11. Ann Genet (Paris) 23:73–77
Williams DL, Look T, Melvin SL, Roberson PK, Dahl G, Flake T, Stass S (1984) New chromosomal translocations correlate with specific immunophenotypes of childhood acute lymphoblastic leukemia. Cell 36:101–109
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Lewis, W.H., Goguen, J.M., Powers, V.E. et al. Gene order on the short arm of human chromosome 11: regional assignment of the LDH A gene distal to catalase in two translocations. Hum Genet 71, 249–253 (1985). https://doi.org/10.1007/BF00284584
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DOI: https://doi.org/10.1007/BF00284584