Summary
The intensity of the silver staining of nucleolus organizing regions (NORs) and the frequency of chromosomal associations were studied before and after antithyroid treatment in seven and eight hyperthyroid patients, respectively.
For one patient, the NOR staining was significantly more intense after treatment, while for another the opposite result was found. A higher stainability of chromosome 22 was observed after treatment in six of seven patients. The difference was significant for the group of patients considered as a whole but only for one patient considered separately. Chromosome 22 also showed an increased association frequency. For one patient, the number of large associations and the total number of associating chromosomes was also significantly increased after treatment.
A decrease of associating chromosomes 14 and 21 such as was reported by Nilsson et al. (1975) after antithyroid treatment could not be found in our sample. The possible reason for this difference are discussed.
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References
Back, E., Zang, K. D.: quantitative studies on the arrangement of human metaphase chromosomes. II. Influence of preparation technique on the association pattern of the acrocentric chromosomes. Cytogenetics 8, 304–314 (1969)
Bloom, S. E., Goodpasture, C.: An improved technique for selective silver staining of nucleolar organizer regions in human chromosomes. Hum. Genet. 34, 199–206 (1976)
De Capoa, S., Ferraro, F., Memendez, F., et al.: Ag staining of nucleolar organizer (NO) and its relation to satellite associations. Hum. Genet. 44, 71–78 (1978)
Fialkow, P. J., Uchida, J. A., Hecht, F., Motulsky, A. G.: Increased frequency of thyroid autoantibodies in mothers of patients with Down's syndrome. Lancet 1965 II, 868–870
Hansson, A.: Satellite association in the human metaphase chromosomes. Hereditas 69, 269 (1971)
Hansson, A., Mikkelsen, M.: An increased tendency to satellite association of human chromosome 21: A factor in the aetiology of Down's syndrome. IRCS (Med. Sci.) 2, 1617 (1974)
Hansson, A.: Satellite association in human metaphases. A comparative study of normal individuals, patients with Down syndrome, and their parents. Hereditas 90, 59–83 (1979)
Luchsinger, U., Bühler, e., Mehes, K., Stalder, G.: Satelliten-assoziationen bei autosomalen und gonosomalen Chromosomenabe rrationen und bei Hypothyreosen. Humangenetik 8, 53–61 (1969)
Mattei, J.-F.: Etude génétique des parents d'enfants trisomique 21. Thesis, Faculté de Médicine de Marseille, 1974
Mattevi, M. S., Salzano, F. M.: Effect of sex, age and cultivation time on the number of satellites and acrocentric associations in man. Humangenetik 29, 265–267 (1975)
Mikelsaar, A.-V., Schwarzacher, H. G.: Comparison of silver staining of nucleolar organizer regions in human lymphocytes and fibroblasts. Hum. Gent. 42, 291–299 (1978)
Miller, D. A., Tantravahi, R., Dev, V. G., et al.: Frequency of satellite associations of human chromosomes is correlated with the amount of Ag staining of the nucleolus organizing regions. Am. J. Hum. Genet. 29, 490–502 (1978)
Nilsson, C., Hansson, A., Nilsson, G.: Influence of thyroid hormones on satellite association in man and the origin of chromosome abnormalities. Hereditas 80, 157–166 (1975)
Zang, K. D., Back, E.: Quantitative studies on the arrangement of human metaphase chromosomes. I. Individual features in the association pattern of acrocentric chromosomes of normal males and females. Cytogenetics 7, 455–470 (1968)
Zankl, H., Bernhardt, S.: Combined silver staining of the nucleolus organizing regions and Giemsa banding in human chromosomes. Hum. Genet. 37, 79–80 (1977)
Zankl, H., Zang, K. D.: Quantitative studies on the arrangement of human metaphase chromosomes. VII. The association pattern of acrocentric chromosomes in carriers of Robertsonian translocations and in their relatives with normal karyotype. Hum. Gent. 52, 119–125 (1979)
Zankl, H., Michaelsen, D., Zank, K. D.: Quantitative studies on the arrangement of human metaphase chromosomes. VI. The association pattern of acrocentric chromosomes in patients with trisomy 13. Hum. Genet. 49, 185–189 (1979)
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Parts of this work are included in the doctoral (MD) thesis of CM
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Zankl, H., Mayer, C. & Zang, K.D. Association frequency and silver staining of nucleolus organizing regions in hyperthyroid patients. Hum Genet 54, 111–114 (1980). https://doi.org/10.1007/BF00279059
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DOI: https://doi.org/10.1007/BF00279059