Summary
Lp(a)-lipoprotein of human serum, when isolated by ultracentrifugation and gelfiltration on Sepharose 4 B, may disaggregate into several components either spontaneously or by mild treatment with detergents:
1. A lipoprotein with characteristics of LDL2; 2. a lipid-free protein complex, which reacts immunologically with anti-Lp(a)-sera; 3. albumin and 4. two other protein components, which were also observed in aged LDL2-preparations and have not been characterized further. The Lp(a)-positive protein moiety could be separated from all other components by chromatography on hydroxyl-apatite.
It is concluded that the Lp(a)-lipoprotein represents a complex protein composed of LDL2-lipoprotein, the Lp(a)-protein moiety, and possibly albumin.
Zusammenfassung
Das durch Ultrazentrifugation und Gelfiltration and Sepharose 4 B isolierte Lp(a)-Lipoprotein des menschlichen Serums zerfällt spontan oder nach milder Behandlung mit Detergentien in mehrere Komponenten:
1. Ein Lipoprotein mit den Eigenschaften der LDL2; 2. einen lipidfreien Proteinkomplex, der immunologisch mit Anti-Lp(a)-Antiseren reagiert; 3. Albumin und 4. zwei weitere Proteinkomponenten, die auch in gealterten LDL2-Präparationen beobachtet wurden, die aber hier nicht weiter charakterisiert wurden. Der Lp(a)-positive Proteinanteil konnte von allen anderen Bestandteilen durch Chromatographie an Hydroxylapatit abgetrennt werden.
Es wird geschlossen, daß das Lp(a)-Lipoprotein ein komplexes Protein darstellt, das aus LDL2-Lipoprotein, dem Lp(a)-Protein und möglicherweise Albumin zusammengesetzt ist.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Berg, K.: A new serum type system in man. The Lp-system. Acta path. microbiol. scand. 59, 369 (1963).
—: Similarity in aminoacid composition between human serum lipoproteins and histocompatibility antigens on cell membranes. Clin. Genet. 2, 193 (1971).
Bütler, R., Brunner, E.: A new interpretation of the serum factor “Ag(e)”. Vox Sang. (Basel) 18, 370–372 (1970).
Charlton, R. K., Zmijewski, C. M.: Soluble HL-A7 antigen: localization in the β-lipoprotein fraction of human serum. Science 170, 636–637 (1970).
Ehnholm, C., Garoff, H.: Characterization of the antigenic determinant of Lp(a)-lipoprotein. Protides of the biological fluids. Brugge, Belgium, April-May 1971.
—, Simons, K., Aro, H.: Purification and quantitation of the human plasma lipoprotein carrying the Lp(a) antigen. Biochim. biophys. Acta (Amst.) 236, 431 (1971).
Harvie, N. R., Schultz, J. S.: Studies of Lp-lipoprotein as a quantitative genetic trait. Proc. nat. Acad. Sci. (Wash.) 66, 99 (1970).
Havel, R. J., Eder, H. A., Bragdon, J. H.: The distribution and chemical composition of ultracentrifugally separated lipoproteins in human serum. J. clin. Invest. 34, 1345 (1955).
Lowry, O. H., Rosenbroogh, N. J., Farr, A. L., Randall, R. J.: Protein measurement with the Folin phenol reagent. J. biol. Chem. 193, 265 (1951).
Margolis, S., Langdon, R. G.: Studies on human-serum-β1-lipoprotiens. J. biol. Chem. 241, 458 (1966).
Rider, A. K., Levy, R. I., Fredrickson, D. S.: “Sinking” prebeta lipoprotein and the Lp-antigen. Circulation 42, Suppl. III-10 (1970).
Scanu, A., Granda, L.: Solubilization and properties of the apolipoproteins of the very low-and low-density lipoproteins in human serum. Biochemistry (Wash.) 5, 3301 (1966).
Schreffler, D. C., Schultz, J. S.: Genetics and immunochemistry of the Lp antigens of human serum. 4th International Congress of Human Genetics, Paris, September 6–11, 1971.
Seidel, D., Alaupovic, P., Furman, R. H., McConathy, W. J.: A lipoprotein characterizing obstructive jaundice. II. Isolation and partial characterization of the protein moieties of low density lipoproteins. J. clin. Invest. 49, 2396 (1970).
Simons, K., Ehnholm, C., Renkonen, V., Bloth, B.: Characterization of the Lp(a)-lipoprotein in human plasma. Acta path. microbiol. scand. 78, 459 (1970).
Utermann, G., Wiegandt, H.: Darstellung und Charakterisierung eines Lipoproteins mit Antigenwinksamkeit im Lp-System. Humangenetik 8, 39 (1969).
—: Disk-elektrophoretischer Nachweis des Lp(a)-Proteins in Lipoproteinfraktionen. Humangenetik 11, 66 (1970).
—: Comparative studies of the Lp(a)-lipoproteins and low density lipoproteins of human serum. Hoppe-Seylers Z. physiol. Chem. 352, 938 (1971).
Wiegandt, H., Lipp, K., Wendt, G. G.: Identifizierung eines Lipoproteins mit Antigenwirksamkeit im Lp-System. Hoppe-Seylers Z. physiol. Chem. 349, 489 (1968).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Utermann, G., Lipp, K. & Wiegandt, H. Studies on the Lp(a)-lipoprotein of human serum. Humangenetik 14, 142–150 (1972). https://doi.org/10.1007/BF00273299
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00273299