Abstract
Three G1 cyclins,CLN1,CLN2, andCLN3, have been identified in the budding yeastSaccharomyces cerevisiae. G1 cyclins are essential, albeit functionally redundant, rate-limiting activators of cell cycle initiation. We have isolated dosage-dependent suppressor genes (designatedHMD genes) of the mating defect caused byCLN3-2, a dominant mutation inCLN3,HMD2 andHMD3 are identical toSTE4 andSTE5, respectively,HMD1 is an essential gene that encodes a protein containing a putative RNA binding domain. Overproduction ofHMD1 results in a relatively specific reduction in the level of theCLN3 orCLN3-2 transcript. This reduction occurs subsequent to transcription initiation ofCLN3 since overexpression ofHMD1 did not affect expression of a heterologous transcript from theCLN3 promoter but did result in a reduction ofCLN3 transcript expressed from a heterologous promoter.HMD1 has at least one essential role independent of its effect onCLN3 sinceHMD1 remains essential for viability in the absence of a functionalCLN3 gene.
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Sugimoto, K., Matsumoto, K., Kornberg, R.D. et al. Dosage suppressors of the dominant G1 cyclin mutantCLN3-2: Identification of a yeast gene encoding a putative RNA/ssDNA binding protein. Molec. Gen. Genet. 248, 712–718 (1995). https://doi.org/10.1007/BF02191711
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DOI: https://doi.org/10.1007/BF02191711