Summary
Mixtures of protoplasts of two auxotrophic mutants of Physcomitrella patens, one requiring thiamine (aneurine), the other p-aminobenzoic acid, have been subjected to electrofusion. The protoplasts were aligned in an alternating electric field (500 KHz, 20 V RMS/cm) and induced to fuse by a brief DC pulse (800 V/cm, time constant lms). After culture, first on complete medium and then on selective medium, hybrid plants were obtained at a frequency of 3%. One hybrid with a morphology typical of polyploidy was also observed.
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References
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Communicated by G. Melchers
Dedicated to Professor Georg Melchers to celebrate his 50-year association with the journal
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Watts, J.W., Doonan, J.H., Cove, D.J. et al. Production of somatic hybrids of moss by electrofusion. Molec Gen Genet 199, 349–351 (1985). https://doi.org/10.1007/BF00330279
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DOI: https://doi.org/10.1007/BF00330279