Summary
Properties of an inversion and a deletion mutant of B. subtilis phage SPP1 which arose during cloning are described. The results are related to the biology of this bacteriophage.
In preceding communications from our laboratories (Heilmann and Reeve 1982, Behrens et al. 1983) we reported the properties of genetically engineered SPP1 bacteriophages, which could be used as cloning vehicles in B. subtilis. These phages contain a unique restriction site within a dispensable region of their genomes. In the course of cloning experiments using these phage vectors, we have occasionally observed the appearance of not only the original vector and desired hybrid phages, but also of SPP1 phages which had undergone extensive genomic rearrangements. Properties of two such phages, SPP1 inv1, which was found to contain a large inversion and of SPP1 delV, a deletion mutant, which defines an additional dispensable region of the SPP1 genome, are described in this communication.
This is a preview of subscription content, log in via an institution to check access.
References
Behrens B, Pawlek B, Morelli G, Trautner TA (1983) Restriction and modification in Bacillus subtilis: Construction of hybrid λ and SPP1 phages containing a DNA methyltransferase gene from B. subtilis phage SPR. Mol Gen Genet 189:10–16
Botstein D, Herskowitz I (1974) Properties of hybrids between Salmonella phage P22 and coliphage λ. Nature 251:584–589
Deichelbohrer I, Messer W, Trautner TA (1982) Genome of Bacillus subtilis bacteriophage SPP1: Structure and nucleotide sequence of pac, the origin of packaging. J Virol 42:83–90
Heilmann H, Reeve JN (1982) Construction and use of SPP1v, a viral cloning vector for Bacillus subtilis. Gene 17:91–100
Hilliker S, Botstein D (1976) Specificity of genetic elements controlling regulation of early functions in temperate bacteriophages. J Mol Biol 106:537–566
Morelli G, Fisseau C, Behrens B, Trautner TA, Luh J, Ratcliff SW, Allison DP, Ganesan AT (1979) The genome of B. subtilis phage SPP1: The topology of DNA molecules. Mol Gen Genet 168:153–164
Noyer-Weidner M, Jentsch S, Pawlek B, Günthert U, Trautner TA (1983) Restriction and modification in Bacillus subtilis: DNA methylation potential of the related bacteriophages Z, SPR, SPβ, ϕ3T and ρ11. J Virol 46:446–453
Santos MA, de Lencastre H, Archer LJ (1984) Homology between phages SPP1, 41c, 22a, ρ15 and SF6. J. Gen. Virol. 65:2067–2072
Southern EM (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 98:503–517
Stüber D, Morelli G, Bujard H, Montenegro MA, Trautner TA (1981) Promoter sites in the genome of B. subtilis phage SPP1. Mol Gen Genet 181:518–521
Author information
Authors and Affiliations
Additional information
Communicated by E.K.F. Bautz
Rights and permissions
About this article
Cite this article
Desmyter, A., Reeve, J.N., Morelli, G. et al. Inversion and deletion mutants in Bacillus subtilis bacteriophage SPP1 as a consequence of cloning. Mol Gen Genet 198, 537–539 (1985). https://doi.org/10.1007/BF00332954
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00332954