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Restriction endonuclease BglI as a tool for in vitro reconstruction and recombination of plasmid and bacteriophage genomes

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Summary

The restriction endonuclease BglI produces different individual fragment ends from different cut sites. This property has allowed us to reconstruct efficiently several commonly used plasmid and bacteriophage genomes and a number of recombinant plasmids containing up to seven BglI restriction sites from their constituent BglI fragments. It is demonstrated that in vitro reconstitution from BglI fragments can be used to create, in a simple way, recombinant DNA molecules by recombining in vitro BglI fragments from different mutated or otherwise related genomes. Further applications of the method are discussed.

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References

  • Bickle TA, Ineichen K (1980) The DNA sequence recognised by BglI. Gene 9:205–212

    Google Scholar 

  • Bolivar F, Rodriguez RL, Greene PJ, Betlach MC, Heyneker HL, Boyer HW, Crosa JH, Falkow S (1977) Construction and characterization of new cloning vehicles. II. A multi-purpose cloning system. Gene 2:95–113

    Google Scholar 

  • Brown NL, Smith M (1977) Cleavage specificity of the restriction endonuclease isolated from Haemophilus gallinarum (HgaI). Proc Natl Acad Sci USA 74:3213–3216

    Google Scholar 

  • Burger KJ, Steinbauer J, Röllich G, Kollek R, Goebel W (1981) Copy number control and incompatibility of plasmid R1: Identification of a protein that seems to be involved in both processes. Mol Gen Genet 182:44–52

    Google Scholar 

  • Chang ACY, Cohen SN (1978) Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid. J Bacteriol 134:1141–1156

    Google Scholar 

  • Cohen SN, Chang ACY, Hsu CL (1972) Nonchromosomal antibiotic resistance in bacteria; genetic transformation of Escherichia coli by R factor DNA. Proc Natl Acad Sci USA 69:2110–2114

    Google Scholar 

  • Cohen SN, Chang ACY, Boyer HW, Helling RB (1973) Construction of biologically functional bacterial plasmids in vitro. Proc Natl Acad Sci USA 70:3240–3244

    Google Scholar 

  • Goebel W, Bonewald R (1975) Class of small multicopy plasmids originating from the mutant antibiotic resistance factor R1drd-19B2. J Bacteriol 123:658–665

    Google Scholar 

  • Kelly TJ, Smith HO (1970) A restriction enzyme from Haemophilus influenzae. II. Base sequence of the recognition site. J Mol Biol 51:393–409

    Google Scholar 

  • Kollek R, Oertel W, Goebel W (1980) Site-specific deletion at the replication origin of the antibiotic resistance factor R1. Mol Gen Genet 177:413–419

    Google Scholar 

  • Lautenberger JA, White CT, Haighwood NL, Edgell MH, Hutchison CA (1980) The recognition site of type II restriction enzyme BglI is interrupted. Gene 9:213–231

    Google Scholar 

  • McDonell MW, Simon MN, Studier FW (1977) Analysis of restriction fragments of T7 DNA and determination of molecular weights by electrophoresis in neutral and alkaline gels. J Mol Biol 110:119–146

    Google Scholar 

  • Moses PB, Horiuchi K (1979) Specific recombination in vitro promoted by the restriction endonuclease HgaI. J Mol Biol 135:517–524

    Google Scholar 

  • Ratcliff SW, Luh J, Ganesan AT, Behrens B, Thompson R, Montenegro MA, Morelli G, Trautner TA (1979) The genome of Bacillus subtilis phage SPP1: The arrangement of restriction endonuclease generated fragments. Mol Gen Genet 168:165–172

    Google Scholar 

  • Roberts RJ (1980) Restriction and modification enzymes and their recognition sequences. Nucl Acids Res 8:63–79

    Google Scholar 

  • Rottländer E, Trautner TA (1970) Genetic and transfection studies with B. subtilis phage SP50. I. Phage mutants with restricted growth on B. subtilis strain 168. Mol Gen Genet 108:84–106

    Google Scholar 

  • Smith HO, Wilcox KW (1970) A restriction enzyme from Haemophilus influenzae. I. Purification and general properties. J Mol Biol 51:379–391

    Google Scholar 

  • Sugisaki H (1978) Recognition sequence of a restriction endonuclease from Haemophilus gallinarum. Gene 3:17–28

    Google Scholar 

  • Sutcliffe JG (1979) Complete nucleotide sequence of the Escherichia coli plasmid pBR322. Cold Spring Harbor Symp Quant Biol 43:77–90

    Google Scholar 

  • Van Heuverswyn H, Fiers W (1980) Recognition sequence for the restriction endonuclease BglI from Bacillus globigii. Gene 9:195–203

    Google Scholar 

  • Wilson GA, Young FE (1976) Restriction and modification in the Bacillus subtilis genospecies. In: Schlessinger D (ed) Microbiology 1976. American Society for Microbiology, Washington DC, pp 350–357

    Google Scholar 

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Authors and Affiliations

  1. Institut für Genetik und Mikrobiologie der Universität Würzburg, Röntgenring 11, D-8700, Würzburg, Germany

    Klaus J. Burger & Reinhard Schinzel

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  1. Klaus J. Burger
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  2. Reinhard Schinzel
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Communicated by E. Bautz

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Burger, K.J., Schinzel, R. Restriction endonuclease BglI as a tool for in vitro reconstruction and recombination of plasmid and bacteriophage genomes. Molec Gen Genet 189, 269–274 (1983). https://doi.org/10.1007/BF00337816

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  • DOI: https://doi.org/10.1007/BF00337816

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