Skip to main content
Log in

Isolation and restriction mapping of plasmids containing ribosomal DNA sequences from the rrn B cistron of E. coli

  • Published:
Molecular and General Genetics MGG Aims and scope Submit manuscript

Summary

Recombinant plasmids containing the entire 16S RNA gene from the rrn B cistron of E. coli inserted in Col E1 and pBR322 plasmid vectors have been constructed. These plasmids have been mapped using several restriction endonucleases as well as by DNA-RNA hybridization. These maps reveal previously undetected restriction sites in the rrn B cistron and in Col E1 plasmid DNA.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Armstrong, K.A., Herschfield, V., Helinski, D.R.: Gene cloning and containment properties of plasmid Col E1 and its derivatives. Science 196, 172–174 (1977)

    Google Scholar 

  • Barnes, W.M.: Plasmid detection and sizing in single colony lysates. Science 195, 393–394 (1977)

    Google Scholar 

  • Barnes, W.M.:J. Bacteriol., submitted (1978)

  • Bickle, T.A., Pirotta, V., Imber, R.: A simple general procedure for purifying restriction endonucleases. Nucleic Acids Res. 4, 2561–2572 (1977)

    Google Scholar 

  • Bolivar, F., Rodriguez, R.L., Betlach, M.C., Boyer, H.W.: Construction and characterization of new cloning vehicles I. Ampicillin-resistant derivatives of the plasmid pMB9. Gene 2, 75–93 (1977)

    Google Scholar 

  • Boros, I., Sain, B.: Restriction endonuclease analysis of the transducing bacteriophage lambda rifd 18. Mol. Biol. Rep. 3, 451–457 (1977)

    Google Scholar 

  • Brosius, J., Palmer, M.L., Kennedy, P.J., Noller, H.F.: Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coli. Proc. Natl. Acad. Sci. U.S.A. 75, 4801–4805 (1978)

    Google Scholar 

  • Carbon, P., Ehresmann, C., Ehresmann, B., Ebel, J.P.: The sequence of Escherichia coli ribosomal 16S RNA determined by new rapid gel methods. FEBS Lett. 94, 152–156 (1978)

    Google Scholar 

  • Clewell, D.B.: Nature of Col E1 plasmid replication in E. coli in the presence of chloramphenicol. J. Bacteriol. 110, 667–676 (1972)

    Google Scholar 

  • Cohen, S.N., Chang, A.C.Y., Hsu, L.: Nonchromosomal antibiotic resistance in bacteria: genetic transformation of E. coli by R-factor DNA. Proc. Natl. Acad. Sci. U.S.A. 69, 2110–2114 (1972)

    Google Scholar 

  • Dunn, J.J., Studier, F.W.: T7 early RNAs and Escherichia coli ribosomal RNAs are cut from large precursor RNAs in vivo by ribonuclease III. Proc. Natl. Acad. Sci. U.S.A. 70, 3296–3300 (1973)

    Google Scholar 

  • Ehresmann, C., Stiegler, P., Carbon, P., Ebel, J.P.: Recent progress in the determination of the primary sequence of the 16S RNA of Escherichia coli. FEBS Lett. 84, 337–341 (1977)

    Google Scholar 

  • Filippes, F.M. De: A new method for isolation of a restriction endonuclease from Haemophilus influencae RD. Biochem. Biophys. Res. Commun. 58, 586–596 (1974)

    Google Scholar 

  • Gegenheimer, P., Apirion, D.: Processing of rRNA by RNAase P: Spacer tRNAs are linked to 16S rRNA in an RNAase P, RNAase III mutant strain of E. coli. Cell 15, 527–539 (1978)

    Google Scholar 

  • Glaser, G., Cashel, M.: In vitro transcripts from the rrn B ribosomal RNA cistron originating from two tandem promoters. Cell (in press)

  • Glaser, G., Enquist, L., Cashel, M.: Col E1 cloning of a ribosomal RNA: Promoter region from λ rifd 18 by selection for lambda integration and excision functions. Gene 2, 159–172 (1977)

    Google Scholar 

  • Guerry, P., Le Blanc, D.J., Falkow, S.: General method for the isolation of plasmid deoxyribonucleic acid. J. Bacteriol. 116, 1064–1066 (1973)

    Google Scholar 

  • Hogan, J.J., Noller, H.F.: Altered topography of 16S RNA in the inactive form of Escherichia coli 30S ribosomal subunits. Biochemistry 17, 587–593 (1978)

    Google Scholar 

  • Kirschbaum, J.B., Greenblatt, J., Allet, B., Rochaix, J.-D.: Studies with a λ rif transducing phage carrying the genes for the β and β' subunits of E. coli RNA polymerase. In: RNA polymerase (Losick, R. and Chamberlin, M., eds.), pp. 503–518. New York: Cold Spr. Harb. Lab. Monogr. Series 1976

  • Kirschbaum, J.B., Konrad, E.B.: Isolation of a specialized lambda transducing bacteriophage carrying the beta subunit gene for E. coli ribonucleic acid polymerase. J. Bacteriol. 116, 517–526 (1973)

    Google Scholar 

  • Kiss, I., Slaska, K., Sümegi, J., Udvardy, A., Venetianer, P.: In vitro transcription of ribosomal RNA on phage λ rifd 18 DNA. Biochim. Biophys. Acta 518, 257–266 (1978)

    Google Scholar 

  • Lindahl, L., Jaskunas, S.R., Dennis, P.P., Nomura, M.: Cluster of genes in Escherichia coli for ribosomal proteins, ribosomal RNA, and RNA polymerase subunits. Proc. Natl. Acad. Sci. U.S.A. 72, 2743–2747 (1975)

    Google Scholar 

  • Lindahl, L., Nomura, M.: Analysis of organization of E. coli In: Ribosome and RNA polymerase genes using biochemical methods, Alfred Benzon Symposium IX (N.O. Kjeldgaard and O. Maaloe, eds.), Control of ribosome synthesis, pp. 206–220. Copenhagen: Munksgaard 1975

    Google Scholar 

  • Lund, E., Dahlberg, J.E., Lindahl, L., Jaskunas, S.R., Dennis, P.P., Nomura, M.: Transfer RNA genes between 16S and 23S rRNA genes in rRNA transcription units of E. coli. Cell 7, 165–177 (1976)

    Google Scholar 

  • Modrich, P., Zabel, D.: Eco RI endonuclease: physical and catalytic properties of the homogeneous enzyme. J. Biol. Chem. 251, 5866–5874 (1976)

    Google Scholar 

  • Morgan, E.A., Ikemura, T., Lindahl, L., Fallon, M., Nomura, M.: Some rRNA operons in E. coli have tRNA genes at their distal ends. Cell 13, 335–344 (1978)

    Google Scholar 

  • Mueller, K., Oebbecke, C., Förster, G.: Capacity of ribosomal RNA promoters of E. coli to bind RNA polymerase. Cell 10, 121–130 (1977)

    Google Scholar 

  • Murray, K., Murray, N.E.: Phage lambda receptor chromosomes for DNA fragments made with restriction endonuclease II of Haemophilus influenza and restriction endonuclease I of Escherichia coli. J. Mol. Biol. 98, 551–564 (1975)

    Google Scholar 

  • Nomura, M., Morgan, E.A., Jaskunas, S.R.: Genetics of bacterial ribosomes. Ann. Rev. Genet. 11, 294–347 (1977)

    Google Scholar 

  • Oka, A., Takanami, M.: Cleavage map of colicin E1 plasmid. Nature 264, 193–196 (1976)

    Google Scholar 

  • Pace, N.R.: Structure and synthesis of the ribosomal ribonucleic acid of prokaryotes. Bacteriol. Rev 37, 562–603 (1973)

    Google Scholar 

  • Reiness, G., Yang, H.-L., Zubay, G., Cashel, M.: Effects of guanosine tetraphosphate on cell free synthesis of E. coli ribosomal RNA and other gene products. Proc. Natl. Acad. Sci. U.S.A. 72, 2881–2885 (1975)

    Google Scholar 

  • Roberts, R.J., Breitmeyer, J.B., Tabachnik, N.F., Myers, P.A.: A second specific endonuclease from Haemophilus aegyptius. J. Mol. Biol. 91, 121–123 (1975)

    Google Scholar 

  • Roberts, R.J., Myers, P.A., Morrison, A., Murray, K.: A specific endonuclease from Arthrobacter luteus. J. Mol. Biol. 102, 157–165 (1976)

    Google Scholar 

  • Sgaramella, V.: Enzymatic oligomerization of bacteriophage P22 DNA and of linear simian virus 40 DNA. Proc. Natl. Acad. Sci. U.S.A. 69, 3389–3393 (1972)

    Google Scholar 

  • Shaltiel, S., Ev-el, Z.: Hydrophobic chromatography: use for purification of glycogen synthetase. Proc. Natl. Acad. Sci. U.S.A. 70, 778–781 (1973)

    Google Scholar 

  • Sharp, P.A., Sugden, B., Sambrook, J.: Detection of two restriction endonuclease activities in Haemophilus parainfluenzae using analytical agarose-ethidium bromide electrophoresis. Biochemistry 12, 3055–3063 (1973)

    Google Scholar 

  • Shinnick, T.M., Lund, E., Smithies, O., Blattner, F.R.: Hybridization of labeled RNA to DNA in agarose gels. Nucleic Acids Res. 2, 1911–1929 (1975)

    Google Scholar 

  • Smith, H.O.: A new method for isolation of a restriction enzyme from Haemophilus parainfluenzae. In: Methods in molecular biology, Vol. 7, pp. 71–85. New York: Marcel Dekker 1974

    Google Scholar 

  • Smith, H.O., Nathans, D.: A suggested nomenclature for bacterial host modification and restriction systems and their enzymes. J. Mol. Biol. 81, 419–423 (1973)

    Google Scholar 

  • Southern, E.M.: Detection of specific sequences among DNA fragments separated by gel electrophoresis. J. Mol. Biol. 98, 503–517 (1975)

    Google Scholar 

  • Thomas, M., Davis, R.W.: Studies on the cleavage of bacteriophage lambda DNA with Eco RI restriction endonuclease. J. Mol. Biol. 91, 315–328 (1975)

    Google Scholar 

  • Wilson, E.A., Young, F.E.: Isolation of a sequence-specific endonuclease (Bam I) from Bacillus amyloliquefaciens H. J. Mol. Biol. 97, 123–125 (1975)

    Google Scholar 

  • Young, R.A., Steitz, J.A.: Complementary sequences 1700 nucleotides apart form a ribonuclease III cleavage site in E. coli ribosomal precursor RNA. Proc. Natl. Acad. Sci. U.S.A. 75, 3593–3597 (1978)

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Additional information

Communicated by H.W. Boyer

Rights and permissions

Reprints and permissions

About this article

Cite this article

Palmer, M.L., Raker, M.A., Kennedy, P.J. et al. Isolation and restriction mapping of plasmids containing ribosomal DNA sequences from the rrn B cistron of E. coli . Molec. Gen. Genet. 172, 171–178 (1979). https://doi.org/10.1007/BF00268279

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00268279

Keywords

Navigation