Summary
The ribosomal protein patterns of recessive suppressor strain and parent strain of Saccharomyces cerevisiae were analyzed by two-dimensional polyacrylamide gel electrophoresis. About 30 protein spots were found for ribosomal proteins of small subunit for both mutant and parent strain. These patterns do not differ from each other neither in intensity of staining, nor in mobility of spots. 41 protein spots were found in electrophoregrams of 60S ribosomal proteins both from parent strain and recessive suppressor strain. The electrophoretic picture of the 60S proteins from the parent and mutant strains is similar except the intensity of staining of the L30 spot. This protein is present in 60S subunit of suppressor strain and completely absent or only weakly stained on electrophoregrams of ribosomal proteins of parent strain. The possible relationships between the content of L30 protein and the mechanism of recessive suppression in yeast are discussed.
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Cannon, M., Schindler, D., Davies, Ju.: Methylation of proteins in 60S ribosomal subunits from Saccharomyces cerevisiae. FEBS Letters 75, 187–191 (1977)
Dabbs, E.R.: Correlation between a novel phenotype towards streptomycine and the binding of an additional protein to the ribosome in mutants of E. coli B. Molec. gen. Genet. 158, 55–62 (1977)
Gerlach, W.L.: Genetic properties of some amber-ochre supersuppressors in Saccharomyces cerevisiae. Molec. gen. Genet. 138, 53–63 (1975)
Gerlach, W.L.: Mutational properties of supP amber-ochre supersuppressors in Saccharomyces cerevisiae. Molec. gen. Genet. 144, 213–215 (1976)
Hawthorne, D.C., Leupold, U.: Suppressor mutations in yeast. Curr. Top. Microbiol. Immunol. 64, 1–47 (1974)
Hebert, J., Pierre, M., Loeb, J.E.: Phosphorylation in vitro and in vivo of ribosomal proteins from Saccharomyces cerevisiae. Europ. J. Biochem. 72, 167–174 (1977)
Inge-Vechtomov, S.G.: Suppressors of adenine requirement in yeast. [In Russian.] Genetika (USSR). 2, 22–26 (1965)
Inge-Vechtomov, S.G.: Identification of some coupling groups in Petergovsky genetical lines of yeast. [In Russian.] Genetika (USSR) 7, 113 (1971)
Inge-Vechtomov, S.G., Andrianova, V.M.: Recessive super-suppressors in yeast. [In Russian.] Genetika (USSR) 11, 103–115 (1970)
Inge-Vechtomov, S.G., Simarov, B.V., Soidla, T.R., Kozin, S.A.: Super-suppressor induced interallelic complementation. Z. Vererbungsl. 98, 375–384 (1966)
Kruiswijk, T.: Some characteristics of the ribosomal proteins in yeast. Ph.D. Thesis, Vrije Universiteit, Amsterdam (1977)
Kruiswijk, T., Planta, R.J.: Analysis of the protein composition of yeast ribosomal subunits by two-dimentional polyacrylamide gel electrophoresis. Molec. biol. Reports 1, 409–415 (1974)
Kruiswijk, T., Planta, R.J.: Further analysis of the protein composition of yeast ribosomes. FEBS Letters 58, 102–105 (1975)
Sherton, C.C., Wool, I.G.: Two-dimentional polyacrylamide gel electrophoresis of eucaryotic ribosomal proteins. In: Methods in enzymology (Moldave, K. and Grossman, L., eds.), Vol. XXX, pp. 500–526. New York-London: Academic Press 1974
Smirnov, V.N., Kreier, V.G., Lizlova, L.V., Andrianova, V.M., Inge-Vechtomov, S.G.: Recessive super-suppression in yeast. Molec. gen. Genet. 129, 105–121 (1974)
Smirnov, V.N., Kreier, V.G., Lizlova, L.V., Inge-Vechtomov, S.G.: Recessive suppression and protein synthesis in yeast. FEBS Letters 38, 96–100 (1973)
Smirnov, V.N., Surguchov, A.P., Fominykch, E.S., Lizlova, L.V., Saprygina, T.V., Inge-Vechtomov, S.G.: Recessive nonsensesuppression in yeast: further characterization of a defect in translation. FEBS Letters 66, 12–15 (1976)
Wittmann, H.G.: Two-dimensional polyacrylamide gel electrophoresis for separation of ribosomal proteins. In: Methods in enzymology (Moldave, K. and Grossman, L., eds.), Vol. XXX pp. 497–505. New York-London: Academic Press 1974
Zeijst Ben A.M. Van der, Kool, A.J., Bloemers, H.P.J.: Isolation of active ribosomal subunits from yeast. Europ. J. Biochem. 30, 15–25 (1972)
Zinker, S., Warner, J.R.: The ribosomal proteins of Saccharomyces cerevisiae. Phosphorylated and exchangeable proteins. J. biol. Chem. 251, 1799–1807 (1976)
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Communicated by D. Goldfarb
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Smirnov, V.N., Surguchov, A.P., Smirnov, V.V. et al. Recessive nonsense-suppression in yeast: Involvement of 60S ribosomal subunit. Molec. Gen. Genet. 163, 87–90 (1978). https://doi.org/10.1007/BF00268967
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DOI: https://doi.org/10.1007/BF00268967