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Propeties of plasmids produced by recombination between R factors of groups J and FII

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Summary

Recombinant plasmids have been produced both by transduction of genetic material from FII R factors into Proteus mirabilis strains carrying plasmids of group J and by insertion of a transposon conferring streptomycin and trimethoprim resistances into a J group R factor.

The transposon-carrying derivative and one of the transductants were shown to be members of group J whereas another transductant was shown to be compatible with members of this group. This recombinant plasmid was able to eliminate but not to be eliminable by R factors of group FII. A model for the origin of this anomalous compatibility characteristics is presented based on the assumption that the recombinant plamid carries part but not all of a complex of binding sites for the repressor of replication [Uhlin and Nordström (1975)].

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Authors and Affiliations

  1. Bacteriology Department, Royal Postgraduate Medical School, London, U.K.

    R. W. Hedges, A. E. Jacob, Naomi Datta & J. N. Coetzee

  2. Institute of Pathology, Microbiology Department, University of Pretoria, Republic of South Africa

    R. W. Hedges, A. E. Jacob, Naomi Datta & J. N. Coetzee

Authors
  1. R. W. Hedges
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  2. A. E. Jacob
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  3. Naomi Datta
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  4. J. N. Coetzee
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Communicated by W. Arber

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Hedges, R.W., Jacob, A.E., Datta, N. et al. Propeties of plasmids produced by recombination between R factors of groups J and FII . Molec. Gen. Genet. 140, 289–302 (1975). https://doi.org/10.1007/BF00267320

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  • DOI: https://doi.org/10.1007/BF00267320

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