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Genetic and transfection studies with B. subtilis phage SP 50

II. Temperature sensitive mutants and the establishment of a linkage map

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Summary

27 temperature sensitive mutants of phage SP 50 were isolated after hydroxylamine or nitrous acid mutagenesis, classified by complementation into groups of functional identity, and mapped by two factor crosses. These mutants, together with the plaque type and functional mutants previously isolated by Rottländer (1966), could be arranged in a linear map comprising a total of 2×92,1 recombination units.

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Authors and Affiliations

  1. Department of Molecular Biology and Virus Laboratory, University of California, Berkeley, California, USA

    W. R. Havender & T. A. Trautner

  2. Abt. Trautner, Max-Planck-Institut für Molekulare Genetik, Berlin, Germany

    W. R. Havender & T. A. Trautner

Authors
  1. W. R. Havender
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  2. T. A. Trautner
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Communicated by G. Bertani

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Havender, W.R., Trautner, T.A. Genetic and transfection studies with B. subtilis phage SP 50 . Molec. Gen. Genetics 108, 61–69 (1970). https://doi.org/10.1007/BF00343185

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  • DOI: https://doi.org/10.1007/BF00343185

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