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Dose- and time-dependent functional and structural damage to the colon mucosa byEntamoeba histolytica trophozoite lysates

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Abstract

Analysis of the initial interaction betweenEntamoeba histolytica trophozoites and the large intestine is impossible in humans and very difficult in experimental animals. To circumvent this obstacle we treated the luminal side of full-thickness rabbit colon segments mounted in Ussing-type chambers with trophozoite lysates of theE. histolytica HM1 virulent strain. Exposure to lysates for up to 90 min produced dose- and time-dependent effects on the colon, consisting of (a) increased decay rates for potential difference, short-circuit current, and transmural resistance and (b) mucosal damage ranging from vacuolation at the bases and shortening of epithelial cells to the loss of intercellular junctions, destruction of microvilli, and necrosis of interglandular epithelial zones. This acute model of intestinal amebiasis is sensitive, fast, and reliable.

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Authors and Affiliations

  1. Department of Cell Biology, Centro de Investigación y de Estudios Avanzados del IPN, Ap. postal 14-740, 07000, México, DF, Mexico

    Fernando Navarro-García & Rubén López-Revilla

  2. Department of Experimental Pathology, Centro de Investigación y de Estudios Avanzados del IPN, Ap. postal 14-740, 07000, México, DF, Mexico

    Víctor Tstusumi

Authors
  1. Fernando Navarro-García
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  2. Rubén López-Revilla
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  3. Víctor Tstusumi
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Navarro-García, F., López-Revilla, R. & Tstusumi, V. Dose- and time-dependent functional and structural damage to the colon mucosa byEntamoeba histolytica trophozoite lysates. Parasitol Res 79, 517–522 (1993). https://doi.org/10.1007/BF00931595

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  • DOI: https://doi.org/10.1007/BF00931595

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