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Radiolabeling ofNecator americanus third stage larvae with75Se-methionine

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Abstract

Third stage larvae ofNecator americanus were radiolabeled with75Se-methionine by two methods. Larvae labeled in aqueous cultures contained 188 and 25 cpm per sheathed and ex-sheathed larva, respectively. Larvae labeled in coproculture incorporated 25 and 18 cpm per sheathed and ex-sheathed larva, respectively. All of the label was decayed in 5 days from larvae labeled in aqueous cultures, whereas appreciable amounts of radioactivity were still detectable at day 7 of chase peroid in coproculture labeled larvae.

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Siddiqui, A.A., Lei, Y., Fusco, A.C. et al. Radiolabeling ofNecator americanus third stage larvae with75Se-methionine. Parasitol Res 76, 722–723 (1990). https://doi.org/10.1007/BF00931094

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  • DOI: https://doi.org/10.1007/BF00931094

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