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Histochemical studies on the distribution of alkaline phosphatase, acid phosphatase, 5-nucleotidase and ATPase in various reproductive tissues of certain digenetic trematodes

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Summary

Out of other functions performed by vitellaria in digenetic trematodes, their role in the formation of shell globules and shell membrane of the capsule, as well as in the excretion of iron with the help of vitamin C is very important. The present histochemical work shows the localization of certain enzymes in different parts of the reproductive system of ten species of trematodes viz.: Neopronocephalus triangularis Mehra, 1932; Glossimetra orientalis Mehra, 1937; Orientodiscus lobatus Srivastava, 1938; Eumegacetes artemii Mehra, 1935; Ganeo tigrinus Mehra et Negi, 1928; Encyclometra caudata Dollfus, 1928; Thapariella udaipurensis Gupta and Sharma, 1970; Paradistomoides indicum Narain et Das, 1929; Patagifer wesleyi Verma, 1936; Proalarioides tropidonotus Vidyarthi, 1937 and indicates their functional significance. The hydrolytic enzymes (alkaline phosphatase, acid phosphatase, 5-nucleotidase and ATPase) are suggestive of their involvement in the uptake of certain nutrients, glycogen and lipoprotein being very significant among others. The four enzymes could also be detected in testes, ovary, uterus, cirrus sac and egg shell. The possible functional significance of each enzyme has been discussed.

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References

  • Bogitsh, B. J.: Histochemical observations on Posthodiplostomum minimum. III. Alkaline phosphatase activity in metacercariae and adults. Exp. Parasit. 19, 339–347 (1966)

    Google Scholar 

  • Bogitsh, B. J., Davis, D. A., Nunnally, D. A.: Cytochemical and biochemical observations on the digestives tracts of digenetic trematodes. II. Ultrastructural localization of acid phosphatase in Haematolochus medioplexus. Exp. Parasit. 23, 303–308 (1968)

    Google Scholar 

  • Burton, P. R.: A histochemical study on vitelline cells, egg capsules and Mehlis' gland in the frog lung fluke, Haematolochus medioplexus. J. exp. Zool. 154, 247–258 (1963)

    Google Scholar 

  • Dike, S. C.: Acid phosphatase activity and ferritin incorporation in the caeca of digenetic trematodes. J. Parasit. 55, 111–123 (1969)

    Google Scholar 

  • Duve, C. de: The lysosome concept. In: A. V. S. de Reuck and M. P. Cameron, eds., CIBA Foundation Symposium on Lysosomes. London: J. A. Churchill 1963

    Google Scholar 

  • Gomori, G.: Microscopic histochemistry. Chicago, Illinois: University of Chicago Press 1952

    Google Scholar 

  • Gupta, A. N., Sharma, P. N.: Histoenzymological studies of digenetic trematodes of vertebrates (Series I). Acta morph. neerl. scand. 12, 61–68 (1974)

    Google Scholar 

  • Halton, D. W.: Studies on phosphatase activity in trematodes. J. Parasit. 53, 46–54 (1967)

    Google Scholar 

  • Hardonk, M. J.: 5-nucleotidase. I. Distribution of 5-nucleotidase in tissues of rat and mouse. Histochem. 12, 1–17 (1968)

    Google Scholar 

  • Hardonk, M. J., Koudstaal, J.: 5-nucleotidase. II. The significance of 5-nucleotidase in the metabolism of nucleotides studied by histochemical and biochemical methods. Histochem. 12, 18–28 (1968)

    Google Scholar 

  • Krupa, P. L., Bogitsh, B. J.: Ultrastructural phosphohydrolase activities in Schistosoma mansoni sporocysts and cercariae. J. Parasit. 58, 495–514 (1972)

    Google Scholar 

  • Nimmo-Smith, R. H., Standen, O. D.: Phosphomonoesterases of Schistosoma mansoni. Exp. Parasit. 13, 305–322 (1963)

    Google Scholar 

  • Novikoff, A. B.: Lysosomes in the physiology and pathology of cells; contributions on staining methods. In: CIBA Foundation Symposium on Lysosomes. Boston, Mass.: Little, Brown 1963

    Google Scholar 

  • Novikoff, A. B., Goldfisher, S.: Nucleoside phosphatase activity in Golgi apparatus and its usefulness for cytological studies. Proc. nat. Acad. Sci. (Wash.) 47, 802–810 (1961)

    Google Scholar 

  • Pearse, A. G. E.: Histochemistry. Theoretical and applied, 2nd ed. London: Churchill 1960

    Google Scholar 

  • Racker, E.: Mechanisms of synthesis of adenosine triphosphatase. Advanc. Enzymol. 23, 323 (1961)

    Google Scholar 

  • Smith, J. D., Clegg, J. A.: Egg-shell formation in trematodes and cestodes. Exp. Parasit. 8, 286–323 (1959)

    Google Scholar 

  • Spence, I. M., Silk, M. H.: Ultrastructural studies of the blood fluke Schistosoma mansoni. V. Female reproductive system; a preliminary report. S. Afr. J. med. Sci. 36, 41–50 (1971)

    Google Scholar 

  • Threadgold, L. T.: Electron microscope studies of Fasciola hepatica. IV. The ultrastructural localization of phosphatases. Exp. Parasit. 23, 264–276 (1968)

    Google Scholar 

  • Wachstein, M., Meisel, E.: Histochemistry of hepatic phosphatases at a physiologic pH. Amer. J. clin. Path. 27, 13 (1957)

    Google Scholar 

  • Yoshimura, H.: Physiological study of Clonorchis sinensis. II. Histochemical aspects of the development of the liver fluke in experimentally infected rats. Jap. J. Parasit. 14, 445–451 (1965)

    Google Scholar 

  • Yoshimura, H., Yokogawa, M.: Physiological studies on the lung fluke. III. Histochemical distribution of polysaccharides, nucleic acids and phosphatases in Paragonimus westermani, Kerbert, 1878. Jap. J. Parasit. 7, 363–369 (1958)

    Google Scholar 

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  1. Department of Zoology, University of Udaipur, India

    P. N. Sharma

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  1. P. N. Sharma
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Sharma, P.N. Histochemical studies on the distribution of alkaline phosphatase, acid phosphatase, 5-nucleotidase and ATPase in various reproductive tissues of certain digenetic trematodes. Z. Parasitenk 49, 223–231 (1976). https://doi.org/10.1007/BF00380592

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  • DOI: https://doi.org/10.1007/BF00380592

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