Summary
The neural plates of very early neurula stages of Triturus alpestris were removed, the material which is released from the extracellular space between mesoderm and neural plate to the medium in which the embryos were dissected was isolated and extracted with phenol. The protein isolated from the phenol layer showed neural inducting activity. Proteoglycans isolated from the aqueous layer did not show such inducing activity. These results together with previously published experiments (Wilhelm Roux's Arch 184: 285–299) suggest that a neuralizing factor which is released from the mesoderm acts on the inner surface of the overlying dorsal ectoderm.
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References
Asahi K-I, Born J, Tiedemann He, Tiedemann Hi (1979) Formation of mesodermal pattern by secondary inducing interactions. Wilhelm Roux's Arch 187: 231–244
Becker U, Tiedemann Hi, Tiedemann He (1959) Versuche zur Determination von embryonalem Amphibiengewebe durch Induktionsstoffe in Lösung. Z für Naturforsch 14b: 608–609
Borń J, Grunz H, Tiedemann He, Tiedemann Hi (1980) Biological activity of the vegetalizing factor: Decrease after coupling to polysaccharide matrix and enzymatic recovery of active factor. Wilhelm Roux's Arch 189: 47–56
Bramhall S, Noack N, Wu M, Loewenberg JR (1969) A simple colorimetric method for determination of protein. Anal Biochem 31: 146–148
Dische Z (1947) A new specific color reaction of hexuronic acids. J Biol Chem 167: 189–198
Johansen PG, Marshall RD (1960) Carbohydrates in protein 2. The hexose, hexose amine, acetyl and amide-nitrogen content of hen's-egg albumin. J Biochem 77: 239–247
Johnson KE (1977) Extracellular matrix synthesis in blastula and gastrula stages of normal and hybrid frog embryos. J Cell Sci 25: 313–322
Kaska DD, Triplett EL (1980) Glycoprotein secretion by isolated Rana pipiens gastrula chordamesoderm. Cell Differ 9: 281–290
Mangold O (1923) Transplantationsversuche zur Frage der Spezifität und der Bildung der Keimblätter. Wilhelm Roux's Arch 100: 198–301
Niebel J, Tiedemann Hi, Tiedemann He (1973) Studies on an inhibitor of embryonic differentiation. Characterization by isoelectric focusing and filtration on sephadex. Eur J Biochem 32: 242–246
Ronelle CJM, Morgan WTJ (1955) The determination of glucosamine and galactosamine. Biochem J 77: 239–252
Saxén L, Toivonen S (1962) Primary embryonic induction. Academic Press, New York, p 111
Spiro RG (1966) Analysis of sugars found in glycoproteins. In: Neufeld EN, Ginsburg (eds). Methods in enzymology 8: 3–26. Academic Press, New York, London
Tarin D (1973) Histochemical and enzyme digestion studies on neural induction in Xenopus laevis. Differentiation 1: 109–126
Tiedemann Hi, Born J (1978) Biological activity of vegetalizing and neuralizing inducing factors after binding to BAC-Cellulose and CNBr-Sepharose. Wilhelm Roux's Arch 184: 285–299
Tiedemann He, Becker U, Tiedemann Hi (1961) Über die primären Schritte bei der embryonalen Induktion. Embryologia 6: 204–218
Tiedemann He, Becker U, Tiedemann Hi (1963) Chromatographic separation of a hind-brain inducing substance into mesodermal and neural-inducing subfractions. Biochim Biophys Acta 74: 557–560
Tiedemann He, Tiedemann Hi, Born J (1969) Polyvinylsulfate: Interaction with complexes of morphogenetic factors and their natural inhibitors. Science 164: 1175–1177
Toivonen S, Tarin D, Saxén L, Tarin PJ, Wartiovaara J (1975) Transfilter studies on neural induction in the newt. Differentiation 4: 1–8
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John, M., Janeczek, J., Born, J. et al. Neural induction in amphibians. Wilhelm Roux' Archiv 192, 45–47 (1983). https://doi.org/10.1007/BF00848769
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DOI: https://doi.org/10.1007/BF00848769