Summary
Vegetalising factor was isolated from swimbladder of crusian carp (Carassius auratus) by solubilishing with 8 M urea the precipitate obtained after digesting the swimbladder with collagenase. The urea-soluble fraction vegetalised isolated presumptive ectoderm ofTriturus gastrula and produced both undifferentiated mesodermal and endodermal cells. Brief heating of the fraction changed its capacity to produce organised mesodermal tissues, such as notochord and somite, and the frequency of induction of undifferentiated cells was reduced. By inserting the urea-soluble fraction into the blastocoel of an early gastrula, embryos without epidermis were obtained. Some of the embryos consisted of undifferentiated mesodermal and endodermal cells, but in the remaining ones small fragments of notochord, small numbers of somites and pronephros developed, enclosed by endodermal cells.
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References
Kawakami, I.: Fish swimbladder, an excellent mesodermal inductor. J. Embryol. exp. Morph.36, 315–320 (1976)
Kawakami, I., Sato, A., Osako, N.: Difference in inductive effect of liver tissues with and without perisinusoidal basement membrane. Develop., Growth and Differ18, 259–266 (1976)
Kocher-Becker, U., Tiedemann, H., Tiedemann, H.: Exovagination of newt endoderm: Cell affinities altered by the mesodermal inducing factor. Science147, 167–169 (1965)
Lindahl, P. E.: Über “animalisierte” und “vegetativisierte” Seeigellarven. Roux' Arch. EntwMech. Org.128, 661–682. (1933)
Nieuwkoop, P.D.: Activation and organization of the central nervous system in Amphibians. I. Induction and activation. II. Differentiation and organization. III. Synthesis of a new working hypothesis. J. exp. Zool.120, 1–108 (1952)
Okada, T.S.: Experimental studies on the differentiation of the endodermal organs in Amphibians. I. Significance of the mesenchymatous tissue to the differentiation of the presumptive endoderm. Mem. Coll. Sci. Univ. Kyoto, ser. B.21, 1–6 (1954)
Takata, C., Yamada, T.: Endodermal tissues developed from the isolated newt ectoderm under the influence of guinea pig bone marrow. Embryologia5, 8–20 (1960)
Tanaka, T., Noda, S., Kawakami, I. and Sato, A.: Inductive capacities of glomerular basement membranes and dentine matrix. Develop., Growth and Differ. 267–272 (1976)
Tiedemann, H.: The molecular basis of differentiation in early development of amphibian embryos. In: “Current topics in developmental biology”. (Eds. A. Monroy and A.A. Moscona), Acad. Press (New York and London)1, 85–112 (1966)
Tiedemann, H., Tiedemann, H.: Zur Gewinnung von Induktionsstoffe aus Hühnerembryonen. Experientia8, 320 (1957)
Timpl, R.: Fraktionierung der nicht-kollagenen Komponenten des Gelenkkapselbindewebes. Z. Physiol. Chem.346, 257–272 (1966)
Toivonen, S., Saxén, L.: The simultaneous inducing action of liver and bone-marrow of the guineapig in implantation and explantation experiments with embryos ofTriturus. Exp. Cell. Res. (suppl.)3, 346–357 (1955)
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Kawakami, I., Noda, S., Kurihara, K. et al. Vegetalising factor extracted from the fish swimbladder and tested on presumptive ectoderm ofTriturus embryos. Wilhelm Roux' Archiv 182, 1–7 (1977). https://doi.org/10.1007/BF00848083
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DOI: https://doi.org/10.1007/BF00848083