Summary
Abscission layer formation in the sour cherry (Prunus cerasus L.) during fruit maturation occurred in the transition zone between the fruit and the pedicel. The abscission layer, consisting of 5–8 rows of cells, was first identified by its low affinity for haematoxylin. The walls of cells in the abscission layer contained less total polysaccharides than adjacent cells. The pectins were degraded and the cellulose was partially broken down resulting in cell separation. The Ca level in the abscission zone decreased and Ca and Mg were lost from the walls of cells in the layer during abscission. After the abscission layer formed, cells associated with the layer had a lower capacity to bind 45Ca than cells distal or proximal to the layer.
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References
Barnell, E.: Studies in tropical fruits. V. Some anatomical aspects of fruit fall in two tropical arboreal plants. Ann. Bot., N.S. 3, 78–89 (1939).
Bornman, C. H., A. R. Spurr, and F. T. Addicott: Abscisin, auxin and gibberellin effects on the developmental aspects of abscission in cotton (Gossypium hirsutum). Amer. J. Bot. 54, 125–135 (1967).
Broda, B.: Über die Verwendung von Chinalizarin, Titangelb und Azurblau zum mikro- und histochemischen Magnesiumnachweis in Pflanzengeweben. Mikrokosmos 32, 184 (1939).
Dahl, L. K.: A simple and sensitive histochemical method for calcium. Proc. Soc. exp. Biol. (N.Y.) 80, 474–479 (1952).
Feigl, F.: Tüpfelanalyse, Bd. I, Anorganischer Teil. Frankfurt 1960.
Frey-Wyssling, A.: Die pflanzliche Zellwand. Berlin-Göttingen-Heidelberg: Springer 1959.
Ginzburg, B. Z.: Evidence for a protein gel structure cross linked by metal cations in the intercellular cement of plant tissues. J. exp. Bot. 12, 85–107 (1961).
Goldstein, D., and C. Stark-Mayer: New specific test for calcium. Anal. chim. Acta 19, 437–439 (1958).
Henglein, F. A.: Die Uron- und Polyuronsäuren (Pektin- und Alginsäure). In: Handbuch der Pflanzenphysiologie, Bd. VI, S. 405–478 (W. Ruhland, ed.). Berlin-Göttingen-Heidelberg: Springer 1958.
Holmes, E. J., and M. J. Brown: Use of a silicone rubber (Clear Seal) as a section adhesive, resistant to acid, alkali and heat. Stain Technol. 42, 249–252 (1967).
Horton, R. F., and Daphne J. Osborne: Senescence, abscission and cellulase activity in Phaseolus vulgaris. Nature (Lond.) 214, 1086–1088 (1967).
Ito, A., and A. Fujiwara: Function of calcium in the cell of rice leaves. Plant Cell Physiol. 8, 409–422 (1967).
Jensen, W. A.: Botanical Histochemistry. San Francisco: Freeman 1962.
Johansen, D. A.: Plant Microtechnique. New York and London: McGraw-Hill Book Co., Inc. 1940.
Kishawa, H. K., and C. M. House: Calcium in cells of fresh tissue stained with glyoxal bis(2-hydroxyanil). Stain Technol. 41, 49–55 (1966).
Letham, D. S.: Separation of plant cells with hexametaphosphate and the nature of intercellular bonding. Exp. Cell Res. 27, 352–355 (1936).
MacDaniels, L. H.: Some anatomical aspects of apple flower and fruitsabscission. Proc. Amer. Soc. Hort. Sci. 34, 122–129 (1936).
McCown, M.: Anatomical and chemical aspects of abscission of fruits of the apple. Bot. Gaz. 105, 212–220 (1943).
Morre, D. J.: Cell wall dissolution and enzyme secretion during leaf abscission. Plant Physiol. 43, 1545–1559 (1968).
Osborne, D. J.: Changes in the distribution of pectin methylesterase across leaf abscission zones of Phaseolus vulgaris. J. exp. Bot. 9, 446–457 (1958).
Preston, R. D.: Structural plant polysaccharides. Endeavour 23, 153–159 (1964).
Rasmussen, H. P.: The mode of entry and distribution of aluminium in Zea mays: Electron microprobe x-ray analysis. Planta (Berl.) 81, 28–37 (1968).
Rasmussen, H. P.: Chemical and physiological changes associated with abscission layer formation in the bean (Phaseolus vulgaris L. ev. Contender). Doct. dissert., Michigan State University, East Lansing, 1965.
Rasmussen, H. P., and M. J. Bukovac: A histochemical study of abscission layer formation in the bean. Amer. J. Bot. 56, 69–76 (1969).
Reeve, R. M.: A specific hydroxylamine-ferric chloride reaction for histochemical localization of pectins. Stain Technol. 34, 209–211 (1959).
Setterfield, G., and S. Bailey: Structure and physiology of cell walls. Ann. Rev. Plant Physiol. 12, 35–62 (1961).
Stösser, R.: Über die Ausbildung des Trenngewebes und seine kausale Beziehung zu Fruchtfall und Fruchtentwicklung bei Süßkirschen. Angew. Bot. 41, 194–209 (1967).
Stösser, R., H. P. Rasmussen, and M. J. Bukovac: A histological study of abscission layer formation in cherry fruits during maturation. J. Amer. Soc. Hort. Sci. 94, in press (1969).
Tagawa, T., and J. Bonner: Mechanical properties of the Avena coleoptile as related to auxin and ionic interactions. Plant Physiol. 32, 207–212 (1957).
Wilson, W. C., and C. H. Hendershott: Anatomical and histochemical studies of abscission of oranges. Proc. Amer. Soc. Hort. Sci. 92, 203–210 (1968).
Yager, R. E.: Possible role of pectic enzymes in abscission. Plant Physiol. 35, 157–162 (1960).
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Michigan Agricultural Experiment Station Journal Article No. 4607
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Stösser, R., Rasmussen, H.P. & Bukovac, M.J. Histochemical changes in the developing abscission layer in fruits of Prunus cerasus L.. Planta 86, 151–164 (1969). https://doi.org/10.1007/BF00379823
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DOI: https://doi.org/10.1007/BF00379823