Abstract
The biosynthetic basis for the high rates of ethylene production by the apical region of etiolated pea (Pisum sativum L.) seedlings was investigated. The ethylene precursor 1-aminocyclopropane-1-carboxylic acid (ACC) was quantified in extracts of various regions of seedlings by measuring isotopic dilution of a 2H-labelled internal standard using selected-ion-monitoring gas chromatography/mass spectrometry. The ACC levels in the apical hook and leaves were much higher than in the expanded internodes of the epicotyl. The capacity of excised tissue sections to convert exogenous ACC to ethylene was also much greater in the apical region, reflecting the distribution of soluble protein in the epicotyl.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Abbreviations
- ACC:
-
1-aminocyclopropane-1-carboxylic acid
- FW:
-
fresh weight
- GC/MS:
-
coupled gas chromatography/mass spectrometry
- HPLC:
-
high-performance liquid chromatography
References
Boller, T., Kende, H. (1980) Regulation of wound ethylene synthesis in plants. Nature 286, 259–260
Brown, B.H., Neill, S.J., Horgan, R. (1986) The use of deuterium labelled internal standards for plant hormone quantitation: a cautionary note. Planta 163, 141–143
Burg, S.P., Burg, E.A. (1968) Auxin stimulated ethylene formation: its relationship to auxin inhibited growth, root geotropism and other plant processes. In: Biochemistry and physiology of plant growth substances, pp. 1275–1294, Wightman, F., Setterfield, G., eds Runge Press, Ottawa
Goeschl, J.D., Pratt, H.K. (1968) Regulatory roles of ethylene in the etiolated growth habit of Pisum sativum. In: Biochemistry and physiology of plant growth substances, pp. 1229–1242, Wightman, F., Setterfield, G. eds. Runge Press, Ottawa
Goeschl, J.D., Pratt, H.K., Bonner, B.A. (1967) An effect of light on the production of ethylene and the growth of the plumular portion of etiolated pea seedlings. Plant Physiol. 42, 1077–1080
Guy, M., Kende, H. (1984) Conversion of 1-aminocyclopropane-1-carboxylic acid to ethylene by isolated vacuoles of Pisum sativum L. Planta 160, 281–287
Jones, J.F., Kende, H. (1979) Auxin-induced ethylene biosynthesis in subapical stem sections of etiolated seedlings of Pisum sativum L. Planta 146, 649–656
Konze, J.R., Kwiatkowski, G.M.K. (1981) Rapidly induced ethylene formation after wounding is controlled by the regulation of 1-aminocyclopropane-1-carboxylic acid synthesis. Planta 151, 327–330
McGaw, B.A., Horgan, R., Heald, J.K. (1985) Selected ion monitoring/isotope dilution mass spectrometric determination of 1-aminocyclopropane-1-carboxylic acid levels in ripening tomato fruit. Anal. Biochem. 149, 130–135
Saltveit, M.E., Dilley, D.R. (1978) Rapidly induced wound ethylene from excised segments of etiolated Pisum sativum L., cv. Alaska, I. Characterization of the response. Plant Physiol. 61, 447–450
Satler, S.O., Kende, H. (1985) Ethylene and the growth of rice seedlings. Plant Physiol. 79, 194–198
Yu, Y.B., Adams, D.O., Yang, S.F. (1979) Regulation of auxin-induced ethylene production in mung bean hypocotyls. Role of 1-aminocyclopropane-1-carboxylic acid. Plant Physiol. 63, 589–590
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Taylor, J.E., Grosskopf, D.G., McGaw, B.A. et al. Apical localization of 1-aminocyclopropane-1-carboxylic acid and its conversion to ethylene in etiolated pea seedlings. Planta 174, 112–114 (1988). https://doi.org/10.1007/BF00394882
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00394882