Abstract
Guard-cell protoplasts were isolated by enzymic digestion of the epidermis peeled from the abaxial surface of leaves from Commelina communis L. The protoplasts were separated from mesophyll-cell protoplasts and other contaminants by density-gradient centrifugation, and the purity of the preparations carefully and quantitatively assessed by light microscopy. The preparations of guard-cell protoplasts were then compared with mesophyll-cell protoplasts in terms of the activity of photosystem II as assessed by a) the light-induced evolution of oxygen under both steady-state and flashing light and b) the characteristics of photosystem-II chlorophyll fluorescence. In all experiments, clear photosystem-II activity was found in guard-cell protoplasts, although some subtle distinctions between guard-cell and mesophyll-cell protoplasts were found. The contribution of any contaimination by mesophyll-cell chlorophyll to guard-cell-protoplast signals was estimated to be less than 3% in all cases. The results indicate that photosystem II is present and active in guard cells of Commelina.
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Abbreviations
- DCMU:
-
3-(3′, 4′-dichlorophenyl)-1,1-dimethylurea
- GCP:
-
guard-cell protoplasts
- GCC:
-
guard-cell chloroplasts
- MCP:
-
mesophyll-cell protoplasts
- MCC:
-
mesophyllcell chloroplasts
- PS:
-
photosystem
References
Allaway, W.G., Mihhorpe, F.L. (1976) Structure and functioning of stomata. In: Water deficits and plant growth, vol. 4, pp. 57–102, Kozlowski, T.T., ed. Academic Press, New York London
Bradley, P.M. (1978) Production of enucleated plant protoplasts of Allium cepa. Plant Sci. Lett. 13, 287–290
Delieu, T., Walker, D.A. (1972) An improved cathode for the measurement of photosynthetic oxygen by isolated chloroplasts. New Phytol. 71, 201–225
Fitzsimons, P.J., Weyers, J.D.B. (1983) Separation and purification of protoplast types from Commelina communis L. leaf epidermis. J. Exp. Bot. 34, 55–66
Habermann, H.M. (1973) Evidence for two photoreactions and possible involvement of phytochrome in light-dependent stomatal opening. Plant Physiol. 51, 543–548
Hipkins, M.F. (1978) Kinetic analysis of the chlorophyll fluorescence inductions from chloroplasts blocked with 3-(3′, 4′-dichlorophenyl)-1,1-dimethylurea. Biochim. Biophys. Acta 502, 514–523
Izawa, S. (1977) Inhibitors of electron transport. In: Encyclopaedia of plant physiology, N.S., vol. 5: Photosynthesis I, pp. 266–282, Trebst, A., Avron, M., eds. Springer, Berlin Heidelberg New York
Joliot, P., Joliot, A. (1968) A polarographic method for detection of oxygen production and reduction of Hill reagents by isolated chloroplasts. Biochim. Biophys. Acta 153 625–634
Kok, B., Forbush, B., McGloin, M. (1970) Co-operation of charges in photosynthetic O2 evolution I. A linear four-step mechanism. Photochem. Photobiol. 11, 457–475
Krause, G.H., Vernotte, C., Briantais, J.-M. (1982) Photoinduced quenching of chlorophyll fluorescence in intact chloroplasts and leaves. Resolution into two components. Biochim. Biophys. Acta 679, 116–124
Kuiper, P.J.C. (1964) Action spectrum of stomatal movement in epidermis tissue of Senecio odoris Defl. Plant Physiol. 39, 952–955
Lavorel, J., Etienne, A.-L. (1977) In vivo chlorophyll fluorescence. In: Topics in photosynthesis, vol. 2: Primary processes of photosynthesis, pp. 203–268, Barber, J., ed. Elsevier, Amsterdam
Lurie, S. (1977) Photochemical properties of guard cell chloroplasts. Plant Sci. Lett. 10, 219–222
Mansfield, T.A., Travis, A.J., Jarvis, R.G. (1981) Responses to light and carbon dioxide. In: Stomatal physiology, pp. 119–135, Jarvis, P.G., Mansfield, T.A., eds. Cambridge University Press, Cambridge
Melis, A., Zeiger, E. (1982) Chlorophyll a fluorescence transients in mesophyll and guard cells. Plant Physiol. 69, 642–647
Morison, J.T.L., Jarvis, P.G. (1983) Direct and indirect effects of light on stomata. II. In Commelina communis L. Plant Cell Environ. 6, 103–109
Ogawa, T., Grantz, D., Boyer, J., Govindjee (1982) Effects of cations and abscisic acid on chlorophyll a fluorescence in guard cells of Vicia faba. Plant Physiol. 69, 1140–1144
Outlaw, W.H., Mayne, B.C., Zenger, V.E., Manchester, J. (1981) Presence of both photosystems in guard cells of Vicia faba. Plant Physiol. 67, 12–16
Papageorgiou, G. (1975) Chlorophyll fluorescence: an intrinsic probe of photosynthesis. In: Bioenergetics of photosynthesis, pp. 319–371, Govindjee, ed. Academic Press, New York London
Radmer, P., Cheniae, G. (1977) Mechanism of oxygen evolution. In: Primary processes of photosynthesis. Topics in photosynthesis, vol. 2, pp. 308–348, Barber, J., ed. Elsevier, Amsterdam
Raghavendra, A.S., Rao, I.M., Das, V.S.R. (1976) Characterisation of ABA inhibition of stomatal opening in isolated epidermal strips. Plant Sci. Lett. 6, 111–115
Raschke, K. (1979) Movements of stomata. In: Encyclopaed of plant physiology N.S., vol. 7: Physiology of movement. pp. 383–447, Haupt, W., Feinleib, M.E., eds. Springer, Berlin Heidelberg New York
Roth-Bejerano, N., Itai, C. (1981) Involvement of phytochrome in stomatal movement: effect of blue and red light. Physiol. Plant. 52, 201–206
Schnabl, H., Hampp, R. (1980) Vicia guard cell protoplasts lack photosystem II activity. Naturwissenschaften 67, 465–466
Shimizaki, K., Gotow, K., Kondo, N. (1982) Photosynthetic properties of guard cell protoplasts from Vicia faba L. Plant Cell Physiol. 23, 871–879
Stokes, D.M., Walker, D.A. (1971) Phosphoglycerate as a Hill oxidant in a reconstituted chloroplast system. Plant Physiol. 48, 163–165
Strain, H.H., Cope, B.T., Svec, W.A. (1971) Analytical procedures for the identification, estimation and investigation of the chlorophylls. Methods Enzymol. 23, 452–476
Weyers, J.D.B., Fitzsimons, P.J. (1982) The non-osmotic volume of Commelina guard cells. Plant Cell Environ. 5, 417–421
Weyers, J.D.B., Fitzsimons, P.J., Mansey, G.M., Martin, E.S. (1983) Guard cell protoplasts—aspects of work with an important new research tool. Physiol. Plant. 58, 331–339
Weyers, J.D.B., Travis, A.J. (1981) Selection and preparation of leaf epidermis for experiments on stomatal physiology. J. Exp. Bot. 32, 837–850
Zeiger, E. (1983) The biology of stomatal guard cells. Annu. Rev. Plant Physiol. 34, 441–475
Zeiger, E., Armond, P., Melis, A. (1981a) Fluorescence properties of guard cell chloroplasts. Plant Physiol. 67, 17–20
Zeiger, E., Field, C., Mooney, H.A. (1981b) Stomatal opening at dawn: possible roles of the blue-light response in nature. In: Plants and the daylight spectrum, pp. 391–407, Smith, H., ed. Academic Press, New York London
Zeiger, E., Hepler, P.K. (1976) Production of guard cell protoplasts from onion and tobacco. Plant Physiol. 58, 492–498
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Hipkins, M.F., Fitzsimons, P.J. & Weyers, J.D.B. The primary processes of photosystem II in purified guard-cell protoplasts and mesophyll-cell protoplasts from Commelina communis L.. Planta 159, 554–560 (1983). https://doi.org/10.1007/BF00409145
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DOI: https://doi.org/10.1007/BF00409145