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Auxin-stimulated ATPase in membrane fractions from pumpkin hypocotyls (Cucurbita maxima L.)

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Abstract

Membrane fractions from Cucurbita maxima hypocotyls were isolated in a medium which inhibits the action of endogenous phospholipases. After removal of soluble phosphatases by Sepharose 2B-CL column chromatography, an auxin-stimulated ATPase activity was found in membrane fractions from linear sucrose gradients. In the presence of 10-4 M phenylacetic acid (PAA), the stimulation by indol-3-acetic acid (IAA) exhibited a bimodal concentration dependence with maximal stimulation of about 50% at 10-6 M IAA. Without PAA, only a high concentration of 10-4 M IAA was stimulatory, whereas 10-6 M IAA had no apparent effect and 10-8 M IAA exhibited weak inhibition. PAA alone had only weak or no effects. The effects of IAA must be considered as hormone-specific. The ATPase activity in the presence of 10-4 M PAA was activated only by 2,4-dichlorophenoxyacetic acid (2,4-D), an active auxin analogue, but not by the inactive stereoisomers, 2,3-D and 3,5-D. Comparison with marker enzyme profiles suggested that part of the auxin-stimulated ATPase was localized on plasma membranes as well as other compartments. Thus, the auxin-stimulated ATPase may become a useful tool in the investigation of the mechanism of action of auxin.

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Abbreviations

2,4-D:

2,4-dichlorophenoxyacetic acid

2,3-D:

2,3-dichlorophenoxyacetic acid

3,5-D:

3,5-dichlorophenoxyacetic acid

IAA:

indol-3-acetic acid

PAA:

phenylacetic acid

MES:

(2-(N-morpholino))-ethanesulfonic acid

EDTA:

ethylenediamine tetraacetic acid

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  1. Botanisches Institut, Universität Bonn, Venusbergweg 22, D-5300, Bonn 1, Federal Republic of Germany

    Günther F. E. Scherer

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Scherer, G.F.E. Auxin-stimulated ATPase in membrane fractions from pumpkin hypocotyls (Cucurbita maxima L.). Planta 151, 434–438 (1981). https://doi.org/10.1007/BF00386536

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  • DOI: https://doi.org/10.1007/BF00386536

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