Abstract
The tissue localization was analysed of collagen X during human fetal and juvenile articular cartilagebone metamorphosis. This unique collagen type was found in the hypertrophic cartilage zone peri- and extracellularly and in cartilage residues within bone trabeculae. In addition, occasionally a slight intracellular staining reaction was found in prehypertrophic proliferating chondrocytes and in chondrocytes surrounding vascular channels. A slight staining was also seen in the zone of periosteal ossification and occasionally at the transition zone of the perichondrium to resting cartilage. Our data provide evidence that the appearance of collagen X is mainly associated with cartilage hypertrophy, analogous to the reported tissue distribution of this collagen type in animals. In addition, we observed an increased and often “spotty” distribution of collagen X with increasing cartilage “degeneration” associated with the closure of the growth plate. In basal hypertrophic cartilage areas, a co-distribution of collagens II and X was found with very little and “spotty” collagen III. In juvenile cartilage areas around single hypertrophic chondrocytes, co-localization of collagens X and I was also detected.
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Capasso O, Gionti E, Pontarelli G, Ambesi-Impiobato FS, Tajana G, Cancedda R (1982) The culture of chick embryo chondrocytes and the control of their differentiated functions in vitro. Exp Cell Res 142:197–206
Cordell JL, Falini B, Erber WN, Ghosh AK, Abdulaziz Z, MacDonald S, Pulford AF, Stein H, Mason DY (1984) Immunoenzymatic labeling of monoclonal antibodies using immune complexes of alkaline phosphatase and monoclonal anti-alkaline phosphatase. J Histochem Cytochem 32:219–225
Gibson GJ, Schor SL, Grant ME (1982) The identification of a new cartilage collagen synthesized by chondrocytes cultured within collagen gels. Connect Tissue Res 9:207–208
Gibson GJ, Bearman CH, Flint MH (1986) The immunoperoxidase localization of type X collagen in chick cartilage and lung. Coll Relat Res 6:163–184
Hsu SM, Raine L, Fanger H (1981) A comparative study of the peroxidase-antiperoxidase method and an avidin-biotin complex method for studying polypeptide hormones with radio immunoassay antibodies. Am J Clin Pathol 75:134–139
Iyama KI, Ninomiya Y, Olsen BR, Linsenmayer TF, Trelstad RL, Hayashi M (1991) Spatiotemporal pattern of type X collagen gene expression and collagen deposition in embryonic chick vertebrae undergoing endochondral ossification. Anat Rec 229:462–472
Kirsch T, Mark K von der (1990) Isolation of bovine type X collagen and immunolocalization in growth-plate cartilage. Biochem J 265:453–459
Kirsch T, Mark K von der (1991a) Isolation of human type X collagen and immunolocalization in fetal human cartilage. Eur J Biochem 196:575–580
Kirsch T, Mark K von der (1991b) Ca-binding properties of type X collagen. FEBS Lett 294:149–152
Linsenmayer TF, Gibney E, Schmid TM (1986) Segmental appearance of type X collagen in the developing avian notochord. Dev Biol 113:467–473
Mark K von der (1986) Differentiation, modulation and dedifferentiation of chondrocytes. In: Kühn K, Krieg T (eds) Connective tissue: biological and clinical aspects. Karger, Basel, pp 272–315
Mark K von der, Mark H von der, Gay S (1976) Study of differential collagen synthesis during development of the chick embryo by immunofluorescence. Dev Biol 48:237–249
Mark K von der, Kirsch T, Aigner T, Reichenberger E, Nerlich A, Weseloh G, Stöß H (1992) The fate of chondrocytes in osteoarthritic cartilage: regeneration, dedifferentiation or hypertrophy? In: Kuettner KE, Schleyerbach R, Peyron JG, Hascall VC (eds) Articular cartilage and osteoarthritis. Raven Press, New York, pp 221–234
Nerlich A, Schleicher E (1991) Immunohistochemical localization of extracellular matrix components in human diabetic glomerular lesions. Am J Pathol 139:889–899
Nerlich A, Wiest I, Kantimm S, Brenner R, Mark K von der (1991) Die immunhistochemische Analyse der normalen und pathologischen Knochen- und Knorpelmatrix als Methode in der Osteologie. In: Werner E, Matthias HH (eds) Osteologie — interdisziplinär. Springer, Heidelberg Berlin New York, pp 41–45
Ninomiya Y, Gordon M, Rest M van der, Schmid TM, Linsenmayer TF, Olsen BR (1986) The developmentally regulated type X collagen gene contains a long open reading frame without introns. J Biol Chem 261:5041–5050
Schmid TM, Conrad HE (1982) A unique low molecular weight collagen secreted by cultured chick embryo chondrocytes. J Biol Chem 257:12444–12450
Schmid TM, Linsenmayer TF (1985a) Immunohistochemical localization of short chain cartilage collagen (type X) in avian tissues. J Cell Biol 100:598–605
Schmid TM, Linsenmayer TF (1985b) Developmental acquisition of type X collagen in the embryonic chick tibiotarsus. Dev Biol 107:373–381
Schmid TM, Linsenmayer TF (1987) Type X collagen. In: Mayne R, Burgeson RE (eds) Structure and function of collagen types. Academic Press, New York London, pp 223–259
Schmid TM, Popp RG, Linsenmayer TF (1990) Hypertrophic cartilage matrix. Ann New York Acad Sci 580:64–73
Summers TA, Irwin MH, Mayne R, Balian G (1988) Monoclonal antibodies to type X collagen. J Biol Chem 263:581–587
Timpl R, Wick G, Gay S (1977) Antibodies to distinct types of collagens and procollagens and their application in immunohistology. J Immunol Methods 18:165–175
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Nerlich, A.G., Kirsch, T., Wiest, I. et al. Localization of collagen X in human fetal and juvenile articular cartilage and bone. Histochemistry 98, 275–281 (1992). https://doi.org/10.1007/BF00270010
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DOI: https://doi.org/10.1007/BF00270010