Summary
Osteoclasts (OCs) and other cells of the mononuclear phagocyte system possess receptors for adhesive proteins present in the extracellular matrix. The antigenic phenotype of OCs and foreign body giant cells (FBGCs) was investigated for the presence of several integrin molecules and other largely platelet-associated antigens involved in cell adhesion reactions. Both OCs and FBGCs expressed the α-chains of the vitronectin receptor (CD51) and of the VLA-2 (CDw49b) and VLA-4 (CDw49d) molecules as well as their respective β-chains, gpIIIa (CD61) and CD29. OCs and FBGCs also expressed CD9 and CD55 (DAF-Decay Accelerating Factor) and strongly reacted with antibodies directed against fibrinogen, fibronectin and vitronectin; the latter are ligands for several of the above matrix protein receptors. The data suggest that cell-cell and cell-matrix interactions involving adhesive proteins may be important in OC and FBGC function.
Similar content being viewed by others
References
Athanasou NA, Quinn J (1990) Immunophenotypic differences between osteoclasts and macrophage polykaryons: immunohistological distinction and implications for osteoclast ontogeny and function. J Clin Pathol 43:997–1003
Athanasou NA, Heryet A, Quinn J, Gatter KC, Mason DY, McGee JO'D (1986) Osteoclasts contain macrophage and megakaryocyte antigens. J Pathol 150:239–246
Athanasou NA, Quinn J, Heryet A, McGee JO'D (1988) Localization of platelet antigens and fibrinogen on osteoclasts. J Cell Sci 89:115–122
Athanasou NA, Quinn J, McGee JO'D (1988) Immunocytochemical analysis of the human osteoclast: phenotypic relationship to other marrow derived cells. Bone Miner 3:317–333
Athanasou NA, Quinn J, Horton MA, McGee JO'D (1990) New sites of cellular vitronectin receptor immunoreactivity detected with osteoclast-reacting monoclonal antibodies 13C2 and 23C6. Bone Mineral 8:7–22
Bouchiex C, Soria C, Mirshahi M, Soria J, Perrot JY, Fournie FN, Ballard N, Rosenfeld C (1983) Characteristics of platelet aggregation induced by the monoclonal antibody ALB6 (acute lymphoblastic leukaemia antigen p24). Inhibition of aggregation by ALB6 Fab. FEBS Lett 161:289–295
Chambers TJ (1980) The cellular basis of bone resorption. Clin Orthop 151:283–293
Davey FR, Cordell JL, Erber WN, Pulford KA, Gatter KC, Mason DY (1988) Monoclonal antibody (Y1/82A) with specificity towards blood monocytes and tissue macrophages. J Clin Pathol 41:753–758
Davies J, Warwick J, Totty N, Philp R, Helfrich M, Horton MA (1989) Osteoclast functional antigen, implicated in the regulation of bone resorption is biochemically related to the vitronectin receptor. J Cell Biol 109:1817–1826
Dotey CK, Bick KL (1977) Ultrahistochemical analysis of glycosaminoglycan hydrolysis in the rat periodontal ligament 1. Calcif Tissue Res 24:135–141
Foon KA, Todd RF (1986) Immunologic classification of leukemia and lymphoma. Blood 68:1–31
Furth R van (1980) Cells of the mononuclear phagocyte system: nomenclature in terms of sites and conditionsh. In: Furth R van (ed) Mononuclear phagocytes. Martinus Nijhoff, The Hague, pp 1–30
Gatter KC, Falini B, Mason DY (1984) The use of monoclonal antibodies in histopathological diagnosis. In: Anthony PP, MacSween RNM (eds) Recent advances in histopathology, no 12. Churchill Livingstone, Edinburgh, pp 35–67
Ginsberg MH, Loftus JC, Plow EF (1988) Cytoadhesions, integrins and platelets. Thromb Haemost 59:1–6
Gothlin G, Ericsson JLE (1976) The osteoclast. Clin Orthop 120:201–231
Hato T, Ikeda K, Yasukow M, Watanabe A, Kobayashi Y (1988) Exposure of platelet fibrinogen receptors by a monoclonal antibody to CD9 antigen. Blood 72:224–229
Hemler ME (1988) Adhesive protein receptors on hematopoietic cells. Immunol Today 4:109–113
Horton MA, Davies J (1989) Adhesion receptors in bone. J Bone Min Res 4:803–808
Hynes RO (1981) Integrins: a family of cell surface receptors. Cell 48:549–554
Kanamura A, Okuda K, Ueda E, Kitani T, Kinoshita T, Nagai K (1988) Different distribution of decay accelerating factor on haemopoietic progentors from normal individuals and patients with paroxysmal nocturnal hemoglobinuria. Blood 957:399–410
Kelly PMA, Bliss E, Morton JA, Burns J, McGee JO'D (1988) Monoclonal antibody EBM/11: high cellular specificity for human macrophages. J Clin Pathol 41:510–515
Kinoshita T, Medof ME, Silber R, Nussenweg V (1985) Distribution of decay accelerating factor in the peripheral blood of normal individuals and patients with paroxysmal nocturnal hemoglobinuria. J Exp Med 165:75–92
Knapp W, Dorken B, Rieber P, Schmidt RE, Stein H, Borne AEGK von den (1989) Leucocyte typing IV. University Press, Oxford
Lasser A (1983) The mononuclear phagocytic system. Human Pathol 14:108–126
Mariano M, Spector WG (1974) The formation and properties of macrophage polykaryons (inflammatory giant cells). J Pathol 113:1–19
Marks SC (1983) The origin of osteoclasts. J Oral Pathol Med 12:226–256
Mundy GR, Roodman GD (1985) Osteoclast ontogeny and function. In: Peck WA (ed) Bone and mineral research, no. 5. Elsevier, Amsterdam, p 209
Phillips DR, Charo IF, Parise LV, Fitzgerald LA (1988) The platelet membrane glycoprotein IIb-IIIa complex. Blood 71:831–843
Plow EF, Loftus JC, Levine EG, Fair DS, Dixon D, Forsyth J, Ginsberg MH (1986) Immunologic relationship between platelet membrane glycoprotein IIb/IIIa and cell surface molecules expressed by a wide variety of cells. Proc Natl Acad Sci USA 83:6002–6006
Revell PA (1982) Tissue reactions to joint prostheses and the products of wear and corrosion. In: Berry CL (ed) Current trends in pathology: bone and joint diseases. Springer, Berlin Heidelberg New York, pp 73–101
Seehafer JC, Tang SC, Slupsky JR, Shaw ARE (1988) The functional glycoprotein CD9 is variably acylated: localization of the variably acylated region to a membrane-associated peptide containing the binding site for the antagonistic monoclonal antibody. 50H. 19. Biochim Biophys Acta 957:399–410
Sutton JS, Weiss L (1966) Transformation of monocytes in tissue culture into macrophages, epithelioid cells and multinucleated giant cells. J Cell Biol 28:303–329
Yasukawa M, Hato T, Inatsuki A, Kobayashi Y (1988) Expression of CD9 (p24) antigen on hematopoietic cells following treatment with phorbol ester. Acta Haematol (Basel) 73:133–136
Zambonin-Zallone A, Teti A, Carano A, Marchisio PC (1988) The distribution of podosomes in osteoclasts cultured on bone laminae: effect of retinol. J Bone Miner Res 3:517–523
Zambonin-Zallone A, Teti A, Grano M, Rubinacci A, Abbadini M, Gaboli M, Marchisio PC (1989) Immunocytochemical distribution of extracellular matrix receptors in human osteoclasts: a beta-3 integrin is colocalised with vinculin and talin in the podosomes of osteoclastoma giant cells. Exp Cell Res 182:645–652
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Quinn, J.M.W., Athanasou, N.A. & McGee, J. Extracellular matrix receptor and platelet antigens on osteoclasts and foreign body giant cells. Histochemistry 96, 169–176 (1991). https://doi.org/10.1007/BF00315989
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00315989