Summary
In the brain of the adult worker bee (Apis mellifica) prolactin-like (PRL) immunoreactive cells were localized in the lateral neurosecretory cell region and the subesophageal ganglion by means of the PAP procedure. These cells emit nerve fibres which pass through the neuropile of the brain to the corpora cardiaca where a great number of immunoreactive axon terminals is present. Tests with antisera against rat pituitary prolactin and human luteinizing hormone were negative. These results indicate that hPRL material is produced in neurosecretory cells of the bee brain and transferred via axons to the corpora cardiaca for storage and subsequent release into haemolymph.
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References
Ammon I, Melani I, Gröschel-Steward U (1966) Insulinktivität bei Schnecken (Helix pomatia). In: Die Pathogenese des Diabetes melitus. Die endokrine Regulation des Fettstoffwechsels. 2. Tagung Dtsch. Diabet. Ges., Wiesbaden
Bhaskar M, Bharathi D, Reddanna P, Lovindappe S (1982) Growth pattern of silkworm larvae Bombyx mori on exposure to prolactin. Indian J Seric 21–22 (0):33–35
Bicker G, Schäfer S, Kingan TG (1985) Muschroom body feedback interneurones in the honeybee show GABA-like immunoreactivity. Brain Res 360:394–397
De Loof A, Verhaert P, Cardoen J (1985) Growth hormone, prolactin and other immunoreactive peptide hormones in the insect Periplaneta americana. Cell Differ (Suppl) 16:78
El-Salhy M, Falkmer S, Kramer KJ, Speirs RD (1983) Immunohistochemical investigations of neuropeptides in the brain, corpora cardiaca and corpora allata of an adult lepidopteran insect, Manduca sexta L. Cell Tissue Res 232:292–317
Hansen GN, Hansen BL, Scharrer B (1988) Diversity of prolactin systems in the insect Leucophaea maderae: Use of antiserum polyclonality for immunocytochemical detection of neuropeptide heterogenity. Cell Tissue Res 252:557–563
Kramer KJ (1980) Insulin-like and glucagon-like hormones in insects. In: Miller TA (ed) Neurohormonal techniques in insects. Springer, Berlin Heidelberg New York, pp 117–136
Kramer KJ (1984) Vertebrate hormones in insects. In: Kramer KJ (ed) Comprehensive insect physiology, biochemistry and pharmacology, vol 7. Pergamon Press, New York, pp 511–536
Le Roith D, Maxine A, Lesniak A, Roth J (1981) Insulin in insects and annelids. Diabetes 30:70–76
Le Roith D, Adamo M, Shemer J, Waldbillig R, Lesniak MA, De Pablo F, Hart C, Roth J (1988) Insulin related material in the nervous system of vertebrates and non-vertebrates: possible extrapancreatic production. Horm Metab Res 20:411–420
Maier V, Grube B, Steiner P, Greischel A, Pfeiffer EF (1982) ACTH, gastrin, glucagon and insulin-like immunoreactivities in the honeybee. Acta Endocrinol (Suppl) 246:36
Maier V, Mezger S, Pfeifle B, Fuchs J, Hang C, Scherbaum W, Herlitzius H, Pfeiffer EF (1986) Isolation and immunohistochemical localization of insulin in the honeybee (Apis mellifica). In: Serrano-Rios, Lefebvre P (eds) Diabetes. Elsevier, Amsterdam, pp 1101–1105
Maier V, Steiner P, Fuchs J, Pfeifle B, Mezger S, Pfeiffer EF (1988) Isolation and partial characterization of insulin in the honeybee (Apis mellifica). Horm Metab Res 20:421–425
Nässel DR, Meyer EP, Klemm N (1985) Mapping the ultrastructure of serotonin-immunoreactive neurons in the optic lobes of three insect species. J Comp Neurol 232:190–204
Noble JN, Goodman LJ (1987) Immunohistochemical localization of gastrin CCK-like peptide in the brain of the honeybee. In: Menzel R, Mercer A (eds) Neurobiology and behaviour of honeybees. Springer, Berlin Heidelberg New York, pp 236–252
Schäfer S, Bicker G (1986a) Common areas of 5-HT- and GABA-like imunoreactive fibres in the visual system of the honeybee. Brain Res 380:368–371
Schäfer S, Bicker G (1986b) Distribution of GABA-like immunoreactivity in the brain of the honeybee (Apis mellifera). J Comp Neurol 246:287–300
Schot LPC, Boer HH, Montaagne-Wajer C (1984) Characterization of multiple immunoreactive neurons in the central nervous system of the pond snail Lymnea stagnalis with different fixatives and antisera observed with the homologous and heterologous antigens. Histochemistry 81:373–378
Schürmann FW, Klemm N (1984) Serotonin-immunoreactive neurons in the brain of the honeybee (Apis mellifera). J Comp Neurol 225:570–580
Sternberger LA (1986) Immunocytochemistry, 3rd edn. John Wiley, New York
Swaab DF, Pool cW, Leeuwen FW Van (1977) Can specifity ever be prooved in immunocytochemical staining? J Histochem Cytochem 25:388–391
Swinnen K, Verhaert P, De Loof A (1987) Immunocytochemical localization of GH, PRL, GHRH and GHIH-like antigenic determinants in the central nervous system of a locust and a fly. Gen Comp Endocrinol 66:47
Veenstra JA, Romberg-Price HM, Schooneveld H, Polak JM (1985) Immunocytochemical localization of peptidergic neurons and neurosecretory cells in the neuroendocrine system of the Colorado potato beetle with antisera to vertebrate regulatory peptides. Histochemistry 82:9–18
Verhaert P, Cardoen J, Vanden Broeck J, De Loof A, Beglay A, Martial JA (1985) Vertebrate cDNA's to peptide hormones hybridize to insect RNA dot blots. Arch Int Physiol Biochim 93 (3):116–117
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This work is part of the Ph. D. thesis of K.P.S.
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Schmid, K.P., Maier, V., Obert, B. et al. Immunocytochemical localization of prolactin-like antigenic determinatns in the neuroendocrine system of the honeybee (Apis mellifica). Histochemistry 91, 469–472 (1989). https://doi.org/10.1007/BF00492517
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DOI: https://doi.org/10.1007/BF00492517