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Renin in the human kidney

Immunohistochemical localization

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Summary

We have localized the enzyme renin (EC 3.4.99.19) in the normal adult human kidney by immunohistology. Serial paraffin sections of kidneys were incubated with renin antisera and then processed by the peroxidase-antiperoxidase method. Renin immunoreactivity was observed in the juxta-glomerular “epithelioid granular cells” (JEG-cells) in the wall of the afferent and rarely of the efferent vessel of the glomerulus. JEG-cells have long cytoplasmic processes penetrating between adjacent cells. This suggests a possible paracrine release of renin. Staining of various segments of the tubular system was shown to be artifactual. The kidney proteins recognized by our anti-human renin antisera had similar characteristics to renin when determined by a combination of gel-electrophoretic and immunologic techniques. Renin immunostaining in the juxtaglomerular apparatus of the human kidney is discrete and reflects the low amount of extractable renin.

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References

  • Adebahr G (1962) Beitrag zur Morphologie der Vasa afferentia und efferentia der juxtamedullären Glomeruli der menschlichen Niere. Z Mikrosk Anat Forsch 68:48–60

    Google Scholar 

  • Brown JJ, Davies DL, Lever AF, Parker RA, Robertson JIS (1965) The assay of renin in single glomeruli in the normal rabbit and the appearance of the juxtaglomerular apparatus. J Physiol (Lond) 176:418–428

    Google Scholar 

  • Bruggmann S, Lutz H (1978) Gel-electrophoresis derived enzyme linked immunosorbent assay (Gedelisa). A new technique for the detection and characterisation of antigens in complex mixtures. In: Knapp W, Holubar K, Wick G (eds) Immunofluorescence and related staining techniques. Elsevier North Holland, Amsterdam, pp 341–342

    Google Scholar 

  • Camilleri JP, Vuong NP, Bariety J, Corvol P, Menard J (1980) Use of specific antiserum for renin detection in human kidney. J Histochem Cytochem 28:1343–1346

    Google Scholar 

  • Chandra S, Skelton FR, Bernardis LL (1964) Separation of renal pressor activity by ultracentrifugation. Lab Invest 13:1192–1198

    Google Scholar 

  • Day RP, Luetscher JA (1975) Biochemical properties of big renin extracted from human plasma. J Clin Endocrinol Metab 40:1085–1093

    Google Scholar 

  • Edelman R, Hartroft PM (1961) Localization of renin in juxta-glomerular cells of rabbit and dog through the use of the fluorescent antibody technique. Circ Res 9:1069–1077

    Google Scholar 

  • Galen FX, Guyenne TT, Devaux C, Menard J, Corvol P, Bariety J, Camilleri JP, N'Goc PW (1980) Purification of human kidney renin and production of highly specific antibodies: application to direct radioimmunoassay of tissue and plasma renin. In: Gross F, Vogel G (eds) Enzymatic release of vasoactive peptides. Raven Press, New York

    Google Scholar 

  • Goldblatt H, Lynch J, Hanzal RF (1934) Studies on experimental hypertension. I. The production of persistent elevation of systolic blood pressure by means of renal ischemia. J Exp Med 59:347–379

    Google Scholar 

  • Goormaghtigh N (1932) Les segments neuro-myo-arteriels juxtaglomerulaires du rein. Arch Biol Liège) 43:575–591

    Google Scholar 

  • Goormaghtigh N (1939) Existence of an endocrine gland in the media of renal arterioles. Proc Soc Exp Biol Med 42:688–714

    Google Scholar 

  • Grube D (1980) Immunoreactivities of Gastrin (G-) cells. II. Non specific binding of immunoglobulins to G-cells by ionic interactions. Histochemistry 66:149–167

    Google Scholar 

  • Haas E, Goldblatt H (1959) Renin content of kidneys in experimental renal and human essential hypertension. Am J Physiol 197:1103–1106

    Google Scholar 

  • Hartroft PM (1963) Juxtaglomerular cells. Circ Res 12:525–538

    Google Scholar 

  • Inagami T, Murakami K, Yokosawa H, Matoba T, Yokosawa N, Takii Y, Misono K (1980) Purification of renin and precusors. In: Gross F, Vogel G (eds) Enzymatic release of vasoactive peptides. Raven Press, New York

    Google Scholar 

  • Jutzler GA (1956) Beitrag zur Morphologie des juxta-glomerulären Apparates bei Drosselungshochdruck. Frankf Z Pathol 67:177

    Google Scholar 

  • Lämmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685

    Google Scholar 

  • Larsson LI, Goltermann N, De Magistris L, Rehfeld JF, Schwartz TW (1979) Somatostatin cell processes as pathways for paracrine secretion. Science 205:1393–1395

    Google Scholar 

  • Meyer D (1972) Morphometrische Untersuchungen am juxtaglomerulären Apparat und Macula densa menschlicher Nieren bei verschiedenen Erkrankungen. Gustav Fischer, Stuttgart (Veröffentlichungen aus der Morphologischen Pathologie, Heft 90, S 1–83)

    Google Scholar 

  • Morgan T, Davis JM (1975) Renin secretion at the individual nephron level. Pflügers Arch 359:23–31

    Google Scholar 

  • Parker RA (1967) Distribution of the changes in the juxta-glomerular apparatus in human renal artery stenosis. Nephron 4:315–325

    Google Scholar 

  • Prinzmetal M, Friedman B (1936) Pressor effects of kidney extracts from patients and dogs with hypertension. Proc Soc Exp Biol Med 35:122–145

    Google Scholar 

  • Robertson AL Jr., Smeby RR, Bumpus FM, Page IH (1966) Production of renin by human juxtaglomerular cells in vitro. Circ Res 18/19 (Suppl I) 131–156

    Google Scholar 

  • Schaffenburg CA, Haas E, Goldblatt H (1960) Concentration of renin in kidneys and angiotensinogen in serum of various species. Am J Physiol 199:788–792

    Google Scholar 

  • Sternberger LA (1979) Immunocytochemistry. 2nd ed. John Wiley, New York

    Google Scholar 

  • Sutherland LE (1970) A fluorescent antibody study of juxta-glomerular cells using the freeze substitution technique. Nephron 7:512–523

    Google Scholar 

  • Swaab DF, Pool CW, van Leeuwen FW (1977) Can specifity ever be proven in immunohistochemical staining? H Histochem Cytochem 25:388–391

    Google Scholar 

  • Taugner Ch, Poulsen K, Hackenthal E, Taugner R (1979) Immunocytochemical localization of renin in mouse kidney. Histochemistry 62:19–27

    Google Scholar 

  • Yokosawa H, Holladay LA, Inagami T, Haas E, Murakamy K (1980a) Human renal renin. Complete purification and characterization. J Biol Chem 155:3498–3502

    Google Scholar 

  • Yokosowa H, Yokosawa N, Inagami T (1980b) Specific antibody to human renal renin and its crossreactivity with inactive human plasma pro-renin. Proc Soc Exp Biol Med 164:466–471

    Google Scholar 

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Authors and Affiliations

  1. Anatomisches Institut der Universität Zürich, Gloriastraße 19, CH-8006, Zürich, Switzerland

    M. R. Celio & T. Inagami

  2. Department of Biochemistry, Vanderbilt University, School of Medicine, 37232, Nashville, TN, USA

    M. R. Celio & T. Inagami

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  1. M. R. Celio
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  2. T. Inagami
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Dedicated to Prof. G. Töndury on the occasion of his 75th birthday

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Celio, M.R., Inagami, T. Renin in the human kidney. Histochemistry 72, 1–10 (1981). https://doi.org/10.1007/BF00496773

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  • DOI: https://doi.org/10.1007/BF00496773

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