Skip to main content
SpringerLink
Log in

Further studies on the precipitative freeze dissolution technique for the localization of inorganic ions

  • Published:
Histochemistry Aims and scope Submit manuscript

Summary

The precipitative freeze dissolution technique (PFD) for the localization of inorganic ions (Van Iren and Bange 1978) was tested further by means of experiments with gelatin models. In several cases dislocation artifacts appeared. By careful comparison of various results, it appeared possible to draw meaningful conclusions.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

PFD:

precipitative freeze dissolution

GDA:

glutar-dialdehyde

mp:

melting point

Δt :

difference between melting point and actual temperature of PFD solutions

References

  • Giusti GV, Fiori A (1969) The histochemical detection of thallium in paraffin embedded tissues by the sulfide-selenium-silver method. Stain Technol 44263–267

    Google Scholar 

  • Korenman IM (1963) Analytical chemistry of thallium. Israel Program Scientific Translations, Jerusalem

    Google Scholar 

  • Sillén GL (1964) Stability constants of metal ion complexes. Section I: inorganic ligands. Special publication no. 17 of the Chemical Society, London

  • Spurr AR (1969) A low viscosity epoxy resin embedding medium for electron microscopy. J Ultrastruct Res 26:31–43

    Google Scholar 

  • Stephen H, Stephen T (1963) Solubilities of organic and inorganic compounds. Pergamon Press, Oxford London New York Paris

    Google Scholar 

  • Van Iren F, Bange GG J (1978) Localization of inorganic ions by precipitative freeze dissolution. Histochemistry 55:81–96

    Google Scholar 

  • Van Iren F, Van der Spiegel A (1975) Subcellular localization of inorganic ions in plant cells by in vivo precipitation. Science 187:1210–1211

    Google Scholar 

  • Van Iren F, Boers-Van der Sluijs FP (1980a) Localization of rubidium in barley roots: a specific role of the root surface in primary absorption. In: Spanswick RM, Lucas WJ, Dainty J (eds) Plant membrane transport. Elsevier/North Holland, Amsterdam, pp 431–432

    Google Scholar 

  • Van Iren F, Boers-Van der Sluijs FP (1980b) Symplasmic and apoplasmic radial ion transport in plant roots: cortical plasmalemmas loose absorption capacity during differentiation. Planta 148:130–137

    Google Scholar 

  • Van Iren F, Boers-Van der Sluijs FP (1980b) Symplasmic and apoplasmic radial ion transport potassium translocation from the root to the shoot of law-salt barley plants. Kinetic and localization studies. Physiol Plant (in press)

  • Voigt GE (1966) Histochemical demonstration of T1. Acta Med Leg Soc 19:17–18

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Plant Nutrition, Botanisch Laboratorium der Rijksuniversiteit, Nonnensteeg 3, NL-2311 VJ, Leiden, The Netherlands

    F. Van Iren & P. Boers-Van der Sluijs

Authors
  1. F. Van Iren
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. P. Boers-Van der Sluijs
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Van Iren, F., Boers-Van der Sluijs, P. Further studies on the precipitative freeze dissolution technique for the localization of inorganic ions. Histochemistry 71, 195–201 (1981). https://doi.org/10.1007/BF00507824

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00507824

Keywords

Search

Navigation