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Improved localization of thiamine pyrophosphatase activity in mounted cryostat sections using gel fixation and gel incubation media

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Summary

The presence of 1% agar in the fixation and substrate solutions for the histochemical demonstration of thiamine pyrophosphatase (4.4 mM TPP; 3.6 mM Pb2+; 0.025 Tris-maleate buffer, pH 7.2) clearly facilitates the localization of the enzyme in Golgi apparatus in cold microtome sections prepared from unfixed specimens.

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References

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Authors and Affiliations

  1. 2nd Department of Pathology of the Medical Faculty UJEP, Children's Hospital, Brno, ČSSR

    Zdeněk Lukáš, Karel Dvořák & Zdeňka Juránková

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  1. Zdeněk Lukáš
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  2. Karel Dvořák
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  3. Zdeňka Juránková
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Lukáš, Z., Dvořák, K. & Juránková, Z. Improved localization of thiamine pyrophosphatase activity in mounted cryostat sections using gel fixation and gel incubation media. Histochemistry 56, 345–347 (1978). https://doi.org/10.1007/BF00495996

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  • DOI: https://doi.org/10.1007/BF00495996

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