Summary
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1.
Tissues examined for the histochemical localization of choline acetyltransferase are best fixed by acetone.
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2.
Topographical identification of choline acetyltransferase by its reaction products is not only substrate-dependent because a slight staining also occurred in the absence of the substrates choline and acetyl-coenzyme in the incubation medium.
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3.
Histochemical localization of choline acetyltransferase was inhibited by sarin but not by DFP or eserine.
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4.
According to Burt and Silver's method cells and cell organelles, of which the enzymatic content is doubtful, were stained.
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5.
Chloroacetylcholine-perchlorate did not inhibit the histochemical localization of choline acetyltransferase.
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6.
The staining of acetylcholinesterase showed a different topographical distribution although both methods were inhibited by sarin.
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Dedicated to Prof. Dr. Gian Töndury, Zürich, on his 70th anniversary.
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Lebbin, C., Waser, P.G. Studies about the specificity of the histochemical localization of choline acetyltransferase. Histochemistry 45, 309–317 (1975). https://doi.org/10.1007/BF00492633
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DOI: https://doi.org/10.1007/BF00492633