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The effect of methanol on granulocyte and horseradish peroxidase quantitatively studied in a film model system

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Summary

Using a model system of polyacrylamide films into which peroxidase had been incorporated, the effect of methanol on histochemically demonstrated enzyme activity could be investigated in quantitative terms. After staining with 3,3′-diaminobenzidine in the presence of hydrogen peroxide it appeared that methanol exerted a marked inhibiting effect on granulocyte peroxidase activity, but did not influence the enzymatic activity of horseradish peroxidase.

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Authors and Affiliations

  1. Department of Histology, Free University, Amsterdam, the Netherlands

    J. G. Streefkerk

  2. Department of Histochemistry and Cytochemistry, University of Leiden, the Netherlands

    M. van der Ploeg

Authors
  1. J. G. Streefkerk
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  2. M. van der Ploeg
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Streefkerk, J.G., van der Ploeg, M. The effect of methanol on granulocyte and horseradish peroxidase quantitatively studied in a film model system. Histochemistry 40, 105–111 (1974). https://doi.org/10.1007/BF00495959

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  • DOI: https://doi.org/10.1007/BF00495959

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