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Demonstration of PAS-positive material in electron microscopy with lead staining

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Summary

1. Two types of lead staining for electron microscopy, with different staining mechanisms, are described.

2. The first type of staining, leading to an increase of the contrast already available, is referred to as “intensifying staining”.

3. The second type of staining leads to the appearance of lead “precipitates” at several sites where PAS-positive material can be expected. This type of staining is therefore referred to as “PAS-like” staining.

4. Preliminary hypotheses for the mechanisms of these different stainings are given.

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Authors and Affiliations

  1. Laboratory for Electron Microscopy, University of Leiden, The Netherlands

    W. Th. Daems & J. -P. Persijn

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  1. W. Th. Daems
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  2. J. -P. Persijn
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Daems, W.T., Persijn, J.P. Demonstration of PAS-positive material in electron microscopy with lead staining. Histochemie 3, 79–88 (1962). https://doi.org/10.1007/BF00736425

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  • DOI: https://doi.org/10.1007/BF00736425

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