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Lipofuscin und Lysosomen im menschlichen Nebenhoden

Fluoreszenzmikroskopische und histochemische Untersuchungen

Lipofuscin and lysosomes in the human epididymis

fluorescence microscopical and histochemical investigations

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Summary

Fluorescence microscopical and histochemical investigations were carried out on lipofuscin and lysosomes in the epididymal epithelium of adults. The results are as follows:

  1. 1.

    The epithelium of the ductuli efferentes contains fluorescent granules of which number and size increase with advancing age. Within the cells of one ductule number, size, and intracellular position of the granules are mainly the same but vary in the cells of different ductules of the same organ. — Very fine fluorescent granules appear with advancing age of the individuum especially in the supranuclear zone of the zylindrical cells of the epididymal duct. Bigger granules which also increase in number with advancing age exist in the supranuclear zone but especially in the nuclear zone. The granulation of the basal zone and the basal cells corresponds to the one found in the two other zones. “Signet-ring like” vacuolar shapes are only found in the epithelium of the epididymal duct.

  2. 2.

    In the epithelium of the ductuli efferentes and of the ductus epididymidis the existence of the following enzyme activities in granules was proved: acid phosphatase, organophosphate resistant esterase, ß-glucuronidase, and acid deoxyribonuclease. The activity of these lysosomal enzymes shows constant différencies in both parts of the organ. — In the epithelium of the ductuli efferentes the fluorescent and the enzyme-containing granules are mainly identical. In the supranuclear zone of the epithelium of the epididymal duct the enzymecontaining granules are more numerous in most cases than the fluorescent granules. The latter belong to the lysosomes, too. — Especially in the ductuli efferentes the enzyme activity of the fluorescent granules shows differencies and can be lower or obviously missing in part of the granules.

  3. 3.

    According to their different number, size, and intracellular position, the histochemical properties of the fluorescent granules are also different. The small ones show a positive reaction with Baker's acid haematein test, sudanophilia in paraffin sections, and a positive PAS-reaction. They are interpreted as being regular lysosomes owning properties of the socalled propigment. The big granules are characterized as lipofuscin granules by their inherent colour, sudanophilia, capability of reduction, basophilia, and acid fastness. Further qualities known from experimental investigations of pigment formation are being described and related to the formation of lipofuscin.

    It is possible to assume stages of development which lead from lysosomes with properties of the so-called propigment to the lipofuscin granules and further to cell inclusions without enzyme activity. According to the age it is possible to observe the stages of this development in different ductuli efferentes of the same organ. These stages show typical differencies in their positional relation to the nucleus. — In the zylindrical cells of the epididymal duct the lipofuscin granules lying in the nuclear zone arise from the lysosomes of the supranuclear zone.

  4. 4.

    The variable lysosome content of the individual ductuli efferentes points to a variable pinocytotic activity of the “vacuolar apparatus” of the epithelial cells. The corresponding differencies in the lipofuscin content of the various ductules have to be seen in relationship with this cell function. — For the lysosomes of the zylindrical cells of the epididymal duct it is possible to assume both participation in pinocytosis and relation to a possible secretoric activity of these cells.

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    Wolfgang Köhl

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Für technische Unterstützung sei Frl. I. Dürr, Frau A. Gadacz, Frl. M. Huber und Frau B. Rottmann gedankt.

Das Manuskript hat der Medizinischen Fakultät der Universität München als Inaugural-dissertation vorgelegen.

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Köhl, W. Lipofuscin und Lysosomen im menschlichen Nebenhoden. Histochemie 16, 236–286 (1968). https://doi.org/10.1007/BF00307852

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