Skip to main content
SpringerLink
Log in

Expression of CD44 isoforms during bleomycin-or radiation-induced pulmonary fibrosis in rats and mini-pigs

  • Original Paper
  • Published:
Histochemistry and Cell Biology Aims and scope Submit manuscript

Abstract

The distribution of CD44s and CD44v molecules in normal and injured lung tissue of rats and minipigs was studied by examining the immunohistochemical binding of monoclonal antibodies against CD44 isoforms. We showed that the expression of CD44v and CD44s varies greatly among different pulmonary fibrosis samples and that some tissues express either enhanced expression of CD44s, particularly in the interstitium and on alveolar macrophages, or very low levels of CD44v in the alveolar epithelium. Normal type II pneumocytes expressed the CD44s and CD44v molecules at the basolateral aspect of the cell. Such localisation favours a role for CD44 in epithelial cell-fibroblast interaction during lung development and repair.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Adamson IYR, Hedgecock C, Bowden DH (1990) Epithelial cell-fibroblast interactions in lung injury and repair. Am J Pathol 137:385–392

    PubMed  Google Scholar 

  • Albelda SM, Smith CW, Ward PA (1994) Adhesion molecules and inflammatory injury. FASEB J 8:504–512

    PubMed  Google Scholar 

  • Asher R, Bignami A (1992) Hyaluronate binding and CD44 expression in human glioblastoma cells and astrocytes. Exp Cell Res 203:80–90

    PubMed  Google Scholar 

  • Bray BA, Sampson PM, Osman M, Giandomenico A, Turino GM (1991) Early changes in lung tissue hyaluronan (hyaluronic acid) and hyaluronidase in bleomycin-induced alveolitis in hamsters. Am Rev Respir Dis 143:284–288

    PubMed  Google Scholar 

  • Culty M, Miyake K, Kincade PW, Sikorski E, Butcher EC, Underhill C, Silorski E [corrected to Sikorski E] (1990) The hyaluronate receptor is a member of the CD44 (H-CAM) family of cell surface glycoproteins [published erratum appears in J Cell Biol (1991) 112(3): following 513]. J Cell Biol 111:2765–2774

    PubMed  Google Scholar 

  • Denning SM, Le PT, Singer KH, Haynes BF (1990) Antibodies against the CD44 p80, lymphocyte homing receptor molecule augment human peripheral blood T cell activation. J Immunol 144:7–15

    PubMed  Google Scholar 

  • Deryckere, F, Gannon F (1994) A one-hour minipreparation technique for extraction of DNA-binding proteins from animal tissues. Biotechniques 16:405

    PubMed  Google Scholar 

  • Flanagan BF, Dalchau R, Allen AK, Daar AS, Fabre JW (1989) Chemical composition and tissue distribution of the human CDw44 glycoprotein. Immunology 67:167–175

    PubMed  Google Scholar 

  • Forsberg UH, Jalkanen S, Schroder J (1989) Assignment of the human lymphocyte homing receptor gene to the short arm of chromosome 11. Immunogenetics 29:405–407

    PubMed  Google Scholar 

  • Fox SB, Fawcett J, Jackson DG, Collins I, Gatter KC, Harris AL, Gearing A, Simmons DL (1994) Normal human tissues, in addition to some tumors, express multiple different CD44 isoforms. Cancer Res 54:4539–4546

    PubMed  Google Scholar 

  • Geyer P (1993) Irradiation technique of the right lung of rats using a 9-MV-photon beam of a lineac. Strahlenther Onkol 169: 734–739

    PubMed  Google Scholar 

  • Günthert U (1993) CD44: a multidute of isoforms with diverse functions. Curr Top Microbiol Immunol 184:47–63

    PubMed  Google Scholar 

  • Jackson DG, Schenker T, Waibel R, Bell JI, Stahel RA (1994) Expression of alternatively spliced forms of the CD44 extracellular-matrix receptor on human lung carcinomas. Int J Cancer Suppl 8:110–115

    PubMed  Google Scholar 

  • Jalkanen S, Aho R, Kallajoki M, Ekfors T, Mortamo P, Gahmberg C, Duijvestijn A, Kalimo H (1989) Lymphocyte homing receptors and adhesion molecules in intravascular malignant lymphomatosis. Int J Cancer 44:777–782

    PubMed  Google Scholar 

  • Juul SE, Kinsella MG, Jackson JC, Truog WE, Standaert TA, Hodson WA (1994) Changes in hyaluronan deposition during early respiratory distress syndrome in premature monkeys. Pediatr Res 35:238–243

    PubMed  Google Scholar 

  • Kasper M, Rudolf T, Verhofstad AA, Schuh D, Müller M (1993a) Heterogeneity in the immunolocalization of cytokeratin-specific monoclonal antibodies in the rat lung: evaluation of three different alveolar epithelial cell types Histochemistry 100:65–71

    PubMed  Google Scholar 

  • Kasper M, Rudolf T, Hahn R, Peterson I, Müller M (1993b) Immuno-and lectin histochemistry of epithelial subtypes and their changes in a radiation-induced lung fibrosis model of the mini-pig. Histochemistry 100:367–377

    PubMed  Google Scholar 

  • Kasper M, Rudolf T, Haase M, Schuh D, Müller M (1993c) Changes in cytokeratin, vimentin and desmoplakin distribution during the repair of irradiation-induced lung injury in adult rats. Virchows Arch [B] 64:271–279

    Google Scholar 

  • Kasper M, Haroske G, Pollack K, Migheli A, Müller M (1993d) HeterogeneousDolichos biflorus lectin binding to a subset of rat alveolar macrophages in normal and fibrotic lung tissue. Acta Histochem 95:1–11

    PubMed  Google Scholar 

  • Kasper M, Schuh D, Müller M (1994a) Immunohistochemical localization of the β subunit of prolyl 4-hydroxylase in human alveolar epithelial cells. Acta Histochem 96:309–313

    PubMed  Google Scholar 

  • Kasper M, Höfer D, Woodcock-Mitchell J, Migheli A, Attanasio A, Rudolf T, Müller M, Drenckhahn D (1994b) Colocalization of cytokeratin 18 and villin in type III alveolar cells (brush cells) of the rat lung. Histochemistry 101:57–62

    PubMed  Google Scholar 

  • Kasper M, Haroske G, Müller M (1994c) Species differences in lectin binding to pulmonary cells: soybean agglutinin (SBA) as a marker of type I alveolar epithelial cells and alveolar macrophages in mini pigs. Acta Histochem 96:63–73

    Google Scholar 

  • Kasper M, Behrens J, Schuh D, Müller M (1995a) Distribution of E-cadherin and Ep-CAM in the human lung during development and after injury. Histochemistry 103:281–286

    PubMed  Google Scholar 

  • Kasper M, Günthert U, Dall P, Kayser K, Haroske G, SchuhD, Müller M (1995b) Distinct patterns of CD44 isoforms during human lung development and after injury in pulmonary fibrosis. Am J Respir Cell Mol Biol 13:648–656

    PubMed  Google Scholar 

  • Kasper M, Haroske G (1996) Alterations of the alveolar epithelium leading to pulmonary fibrosis. Histol Histopathol (in press)

  • Kim H, Yang XL, Rosada C, Hamilton SR, August JT (1994) CD44 expression in colorectal adenomas is an early event occurring prior to K-ras and p53 gene mutation. Arch Biochem Biophys 310:504–507

    PubMed  Google Scholar 

  • Lasky LA, Singer MS, Yednock TA, Dowbenko D, Fennie C, Rodriguez H, Nguyen T, Stachel S, Rosen SD (1989) Cloning of a lymphocyte homing receptor reveals a lectin domain. Cell 56:1045–1055

    PubMed  Google Scholar 

  • Lesley J, Hyman R (1992) CD44 can be activated to function as an hyaluronic acid receptor in normal murine T cells. Eur J Immunol 22:2719–2723

    PubMed  Google Scholar 

  • Li H, Hamou MF, Tribolet N de, Jaufeerally R, Hofmann M, Diserens AC, Van-Meir EG (1993) Variant CD44 adhesion molecules are expressed in human brain metastases but not in glioblastomas. Cancer Res 53:5345–5349

    PubMed  Google Scholar 

  • Liu AY (1994) Expression of CD44 in prostate cancer cells. Cancer Lett 76:63–69

    PubMed  Google Scholar 

  • Mackay CR, Terpe HJ, Stauder R, Marston WL, Stark H, Günthert U (1994) Expression and modulation of CD44 variant isoforms in humans. J Cell Biol 124:71–82

    PubMed  Google Scholar 

  • Mayer B, Jauch KW, Günthert U, Figdor CG, Schildberg FW, Funke I, Johnson JP (1993) De-novo expression of CD44 and survival in gastric cancer. Lancet 342:1019–1022

    PubMed  Google Scholar 

  • Penno MB, August JT, Baylin SB, Mabry M, Linnoila RI, Lee VS, Croteau D, Yang XL, Rosada C (1994) Expression of CD44 in human lung tumors. Cancer Res 54:1381–1387

    PubMed  Google Scholar 

  • Roussel E, Gingras MC, Ro JY, Branch C, Roth JA (1994) Loss of alpha 1 beta 1 and reduced expression of other beta 1 integrins and CAM in lung adenocarcinoma compared with pneumocytes. J Surg Oncol 56:198–208

    PubMed  Google Scholar 

  • Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual, vol 1. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York

    Google Scholar 

  • Schreiber E, Matthias P, Müller MM, Schaffner W (1989) Rapid detection of octamer binding proteins with mini-extracts, prepared from a small number of cells. Nucleic Acids Res 17:6419

    PubMed  Google Scholar 

  • Terpe A, Franke F, Stark H, Gustmann C, Mackay C, Marston W, Günthert U (1993) Occurrence of CD44 and its isoforms under orthological and pathological conditions. Verh Dtsch Ges Pathol 77:276–281

    PubMed  Google Scholar 

  • Terpe HJ, Stark H, Prehm P, Günthert U (1994) CD44 variant isoforms are preferentially expressed in basal epithelial of nonmalignant human fetal and adult tissues. Histochemistry 101:79–89

    PubMed  Google Scholar 

  • Underhill CB, Nguyen HA, Shizari M, Culty M (1993) CD44 positive macrophages take up hyaluronan during lung development. Dev Biol 155:324–336

    PubMed  Google Scholar 

  • Wirth K, Arch R, Somasundaram C, Hofmann M, Weber B, Herrlich P, Matzku S, Zoller M (1993) Expression of CD44 isoforms carrying metastasis-associated sequences in newborn and adult rats. Eur J Cancer 8:1172–1177

    Google Scholar 

  • Yang H, Hutchings A, Binns RM (1993) Preparations and reactivities of anti-porcine CD44 monoclonal antibodies. Scand J Immunol 37:490–498

    PubMed  Google Scholar 

  • Young L, Adamson IY (1993) Epithelial-fibroblast interactions in bleomycin-induced lung injury and repair. Environ Health Perspect 101:56–61

    PubMed  Google Scholar 

  • Zhou DF, Ding JF, Picker LJ, Bargatze RF, Butcher EC, Goeddel DV (1989) Molecular cloning and expression of Pgp-1. The mouse homolog of the human H-CAM (Hermes) lymphocyte homing receptor. J Immunol 143:3390–3395

    PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institute of Pathology, Technical University of Dresden, Fetscherstrasse 74, D-01307, Dresden, Germany

    M. Kasper, A. Bierhaus, D. Schuh & M. Müller

  2. The Babraham Institute, CB2 4AT, Cambridge, UK

    A. Whyte & R. M. Binns

Authors
  1. M. Kasper
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. A. Bierhaus
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. A. Whyte
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. R. M. Binns
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. D. Schuh
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. M. Müller
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kasper, M., Bierhaus, A., Whyte, A. et al. Expression of CD44 isoforms during bleomycin-or radiation-induced pulmonary fibrosis in rats and mini-pigs. Histochem Cell Biol 105, 221–230 (1996). https://doi.org/10.1007/BF01462295

Download citation

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01462295

Keywords

Search

Navigation