Abstract
Stimulation of soluble guanylyl cyclase (SGC) by nitric oxide (NO) results in the generation of cyclic guanosine monophosphate (cGMP). We recently described expression of abundant nitric oxide synthase, the enzyme by which NO is generated froml-arginine, in macula densa cells of rat kidney at the protein and mRNA level. In the present study we looked for possible targets of NO in the kidney. By light and electron microscopy, we applied polyclonal antisera against four subunits (α1,α2, β1β2) of SGC in immunocytochemical studies of frozen sections of rat kidney. We demonstrate the presence of α1-subunit in glomerular podocytes and of β2-subunit in principal cells of the collecting duct. In both cell types a cytosolic localization was evident from ultrastructural analysis. Regarding the collecting duct, NO was shown by other authors to inhibit sodium reabsorption in cultured mouse cortical collecting duct principal cells. In podocytes NO may relax the contractile system of podocyte foot processes, the tone of which has been suggested to counteract the elastic distension of the capillary wall.
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References
Bachmann S, Mundel P (1994) Nitric oxide in the kidney: synthesis, localization, and function. Am J Kid Dis 24:112–129
Cattel V, Cook HT (1993) Nitric oxide: role in the physiology and pathology of the glomerulus. Exp Nephrol 1:265–280
Chevalier RL, Fern RF, Garmey M, El-Dahr SS, Gomez RA, Vente J de (1992) Localization of cyclic GMP after infusion of nitroprusside in the maturing rat. Am J Physiol 262:417–424
Elger M, Drenckhahn D, Nobiling R, Mundel P, Kriz W (1993) Cultured rat mesangial cells contain muscle α-actin not found in vivo. Am J Pathol 142:497–509
Garbers DL (1992) Guanylyl cyclase receptors and their endocrine, paracrine, and autocrine ligands. Cell 71:1–4
Harteneck C, Wedel B, Koesling D, Malkewitz J, Böhme E, Schultz G (1991) Molecular cloning and expression of a new α-subunit of soluble guanylyl cyclase. Interchangeability of the α-subunits of the enzyme. FEBS Lett 292:217–222
Kaissling B, Kriz W (1979) Structural analysis of the rabbit kidney. Adv Anat Embryol 56:1–123
Kasinath BS, Fried TA, Davalath S, Marsden PA (1992) Glomerular epithelial cells synthesize endothelin peptides. Am J Pathol 141:279–283
Koesling D, Böhme E, Schulz G (1991) Guanylyl cyclases, a growing family of signal-transducing enzymes. FASEB J 5:2785–2791
Kriz W, Hackenthal E, Nobiling R, Sakai T, Elger M (1994) A role for podocytes to counteract capillary wall distension. Kidney Int 45:369–376
Lahera V, Salom MG, Fiksen-Olsen MJ, Raij L, Romero JC (1990) Effects of Ng-monomenthyl-l-arginine andl-arginine on acetylcholine renal response. Hypertension 15:659–663
Mundel P, Bachmann S, Bader M, Fischer A, Kummer W, Mayer B, Kriz W (1992) Expression of nitric oxide synthase in kidney macula densa cells. Kidney Int 42:1017–1019
Romero JC, Labera V, Salom MG, Biuondi ML (1992) Role of endothelium-derived relaxing factor nitric oxide on renal function. J Am Soc Nephrol 2:1371–1387
Shake JG, Brandt RC, Daniels BS (1992) Angiotensin II induces actin polymerization within the glomerular filtration barrier: possible role in the local regulation of ultrafiltration. J Am Soc Nephrol 3:568
Sharma R, Lovell HB, Wiegmann TB, Savin VJ (1992) Vasoactive substances induce cytoskeletal changes in cultured glomerular epithelial cells. J Am Soc Nephrol 3:1131–1138
Shultz PJ, Schorer AR, Raij L (1990) Effects of endothelium-derived relaxing factor and nitric oxide on rat mesangial cells. Am J Physiol 258:162–167
Stamler JS, Simon DI, Osborne JA, Mullins ME, Jaraki O, Michel T, Singel DJ, Loscalzo J (1992) S-nitrosylation of proteins with nitric oxide: synthesis and characterization of biologically active compounds. Proc Natl Acad Sci USA 89:444–448
Stoos BA, Carrelero OA, Fahry RD, Scicli G, Garvin JL (1992) Endothelium-derived relaxing factor inhibits transport and increases cGMP content in cultured mouse cortical collecting duct cells. J Clin Invest 89:761–765
Terada Y, Kimio T, Nonoguchi T, Marumo F (1992) Polymerase chain reaction localization of constitutive nitric oxide synthase and soluble guanylyl cyclase messenger RNAs in microdisseceted rat nephron segments. J Clin Invest 90:659–665
Ujiie K, Drewett JG, Yuen PS, Star RA (1993) Differential experssion of mRNA for guanylyl cyclase-linked endothelium-derived relaxing factor receptor subunits in rat kidney. J Clin Invest 91:730–734
Yuen PS, Potter LR, Garbers DL (1990) A new form of guanylyl cyclase is preferentially expressed in rat kidney. Biochemistry 29:10872–10878
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Mundel, P., Bachmann, S., Kriz, W. et al. Immunolocalization of soluble guanylyl cyclase subunits in rat kidney. Histochem Cell Biol 103, 75–79 (1995). https://doi.org/10.1007/BF01464478
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DOI: https://doi.org/10.1007/BF01464478