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Schiff-type reagents in cytochemistry

I. Theoretical and practical considerations

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Zeitschrift für Zellforschung und mikroskopische Anatomie Abt. Histochemie Aims and scope Submit manuscript

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Bibliography

  • Alexander, E. R., andE. J. Underhill: Studies on the mechanism of the Mannich reaction. I. Ethylmalonic acid, a methylnyl compound. J. Amer. chem. Soc.71, 4014–4019 (1949).

    Google Scholar 

  • Atwood, L., andL. Ornstein: Referred to byE. D. De Lamater, A new cytological basis for bacterial genetics. Cold Spr. Harb. Symp. Quant. Biol.16, 381–412 (1951).

    Google Scholar 

  • Barger, J. D., andE. D. De Lamater: The use of thionyl chloride in the preparation of Schiff's reagent. Science108, 121–122 (1948).

    Google Scholar 

  • Barka, T.: Observations on the mechanism of visualization of tissue aldehyde groups using S35 labeled Schiff reagent (abstract). J. Histochem. Cytochem.,1, 289–290 (1959) (Abstract).

    Google Scholar 

  • Bartell, P. D.: The study of organic reaction mechanisms. Organic Chemistry byH. Gilman, vol. III, p. 1–121. New York: John Wiley & Sons 1953.

    Google Scholar 

  • Bauer, H.: Die Feulgensche Nuklealfärbung in ihrer Anwendung auf cytologische Untersuchungen. Z. Zellforsch.15, 225–247 (1932).

    Google Scholar 

  • Block, R. J., E. L. Durrum andG. Zweig: A Manual of Paper Chromatography and Paper Electrophoresis. New York: Academic Press 1955.

    Google Scholar 

  • Bucherer, H. T.: Lehrbuch der Farbenchemie, 2. Aufl. Leipzig: Otto Spamer 1921.

    Google Scholar 

  • Colour Index, V. 3, 4, 2nd edit. Leeds: Chorley & Pickersgill 1957, 1958.

    Google Scholar 

  • Colour Index—Cross Indices. I. Old colour index numbers to new colour index names and numbers. Amer. Dyestuff Rep.46, 610–612 (1957a).

    Google Scholar 

  • II. Old prototype numbers to new colour index names and numbers. Amer. Dyestuff Rep.46, 613–615 (1957b).

    Google Scholar 

  • III. New colour index names and numbers to old names and numbers. Amer. Dyestuff Rep.46, 616–624, 655–673 (1957c).

    Google Scholar 

  • Conn, H. J.: Thionin and its derivatives. Stain Technol.2, 35–40 (1927).

    Google Scholar 

  • Biological Stains. Baltimore: Williams & Wilkins Company 1953.

    Google Scholar 

  • Dürrschnabel, K., u.H. Weil: Über die Einwirkung der schwefligen Säure auf die Triphenylmethan-Farbstoffe. Ber. dtsch. chem. Ges.38, 3492–3496 (1905).

    Google Scholar 

  • Duijn, P. van: A histochemical specific thionin-SO2 reagent and its use in a bicolor method for deoxyribonucleic acid and periodic acid Schiff positive substances. J. Histochem. Cytochem.4, 55–63 (1956).

    Google Scholar 

  • Feigl, F.: Spot tests in organic analysis. New York: Elsevier Publ. Comp. 1956.

    Google Scholar 

  • Feulgen, R., u.H. Rossenbeck: Mikroskopisch-chemischer Nachweis einer Nucleinsäure vom Typus der Thymonucleinsäure und die darauf beruhende elektive Färbung von Zellkernen in mikroskopischen Präparaten. Z. physiol. Chem.135, 203–248 (1924).

    Google Scholar 

  • Glegg, R. E., Y. Clermont andC. P. Le Blond: The use of lead tetraacetate, benzidine, o-dianisidine and a “film test” in investigating the periodic-acid-Schiff technic. Stain Technol.27, 277–305 (1952).

    Google Scholar 

  • Hantzsch, A., u.G. Osswald: Über die Umwandlung von Farbbasen in Pseudoammonium-Hydrate, -Cyanide und -Sulfonsäure. Ber. dtsch. chem. Ges.33, 278–317 (1900).

    Google Scholar 

  • Hartman, M. I.: Personal communication 1958.

  • Himes, M., andL. Moriber: A triple stain for deoxyribonucleic acid, polysaccharides and proteins. Stain Technol.31, 67–70 (1956).

    Google Scholar 

  • Hörmann, H., W. Grassman andG. Fries: Über den Mechanismus der Schiffschen Reaktion. Justus Liebigs Ann. Chem.616, 125–147 (1958).

    Google Scholar 

  • Holmes, W. C.: Subsidiary dyes in methylene blue. Stain Technol.2, 71–73 (1927).

    Google Scholar 

  • Holmes, W. C., andR. W. French: The oxidation products of methylene blue. Stain Technol.1, 17–26 (1926).

    Google Scholar 

  • Hotchkiss, R. D.: A microchemical reaction resulting in the staining of polysaccharide structures in fixed tissue preparations. Arch. Biochem.16, 131–141 (1948).

    Google Scholar 

  • Kasten, F. H.: Chromosomin and the Feulgen reaction. J. Histochem. Cytochem.4, 310–317 (1956a).

    Google Scholar 

  • Stability of the Feulgen-deoxyribonucleic acid absorption curvein situ with variation in nuclear protein content and other factors. J. Histochem. Cytochem.4, 462–470 (1956b).

    Google Scholar 

  • The Feulgen-deoxyribonucleic acid absorption curve in normal and tumorous tissues. J. Histochem. Cytochem.3, 398–402 (1957).

    Google Scholar 

  • Additional Schiff-type reagents for use in cytochemistry. Stain Technol.33, 39–45 (1958a).

    Google Scholar 

  • The Feulgen-DNA absorption curve in situ. Histochemie1, 123–150 (1958b).

    Google Scholar 

  • Kasten, F. H., andV. Burton: A modified Schiff's solution. Stain Technol.34, 289 (1959).

    Google Scholar 

  • Kasten, F. H., V. Burton andP. Glover: New fluorescent Schiff-type reagents for cytochemical detection of polyaldehyde moieties in sections and smears. Nature (Lond.) (in press, 1959).

  • Kasten, F. H., andS. Lofland: Preliminary studies of dye impurities in pyronin B and pyronin Y. J. Histochem. Cytochem.6 394–395 (abstract) (1958).

    Google Scholar 

  • Lessler, M. A.: The nature and specificity of the Feulgen reaction. Arch. Biochem.32, 42–54 (1951).

    Google Scholar 

  • Lubs, H. A.: The Chemistry of Synthetic Dyes and Pigments. New York: Reinhold Publ. Comp. 1955.

    Google Scholar 

  • Ornstein, L., W. Mautner, B. J. Davis andR. Tamura: New horizons in fluorescence microscopy. J. Mt. Sinai Hosp.24, 1066–1078 (1957).

    Google Scholar 

  • Oster, K. A., andJ. G. Oster: The specificity of sex hormones on the tissue aldehyde shift in the rat kidney and of fuchsin sulfurous acid reagent on aldehydes. J. Pharmacol. exp. Ther.87, 306–312 (1946).

    Google Scholar 

  • Ostergren, G.: Chromatin stains of Feulgen type involving other dyes than fuchsin. Hereditas (Lund)34, 510–511 (1948).

    Google Scholar 

  • Pearse, A. G. E.: Histochemistry, Theoretical and Applied. London: J. & A. Churchill 1953.

    Google Scholar 

  • Rafalko, J. S.: A modified Feulgen technic for small and diffuse chromatin elements. Stain Technol.21, 91–93 (1946).

    Google Scholar 

  • Rowe, F. M.: Colour Index. Bradford: Soc. of Dyers & Colourists 1924.

    Google Scholar 

  • Supplement to Colour Index. Bradford: Soc. of Dyers & Colourists 1928.

    Google Scholar 

  • Rumpf, P.: Recherches physico-chimiques sur la reaction colorée des aldéhydes, dite “Reaction de Schiff”. Ann. Chim. (Paris)3, 327–442 (1935).

    Google Scholar 

  • Schiff, H.: Derivate des Rosanilins. Z. Chem.10, 175–176 (1867a).

    Google Scholar 

  • Action des aldehydes sur les alcaloides et les sulfites d'aniline. Bull. Soc. Chim.7, 443–447 (1867b).

    Google Scholar 

  • Schmidt, J. G.: Über das Verhalten einiger organischen Verbindungen zu Fuchsinschwefligersäure. Ber. dtsch. chem. Ges.14, 1848–1851 (1881).

    Google Scholar 

  • Stedman, E., andE. Stedman: The cytological interpretation of the Feulgen reaction. Biochem. J.47, 508–512 (1950).

    Google Scholar 

  • Tomasi, J. A. de: Improving the technic of the Feulgen stain. Stain Technol.11, 137–144 (1936).

    Google Scholar 

  • Venkataraman, K.: The chemistry of synthetic dyes, V 1, V 2. Academic Press 1952.

  • Wertheim, E.: A modified Schiff's solution. J. Amer. chem. Soc.44, 1834–1835 (1922).

    Google Scholar 

  • Wieland, H., u.G. Scheuing: Die fuchsin-schweflige Säure und ihre Farbreaktion mit Aldehyden. Ber. dtsch. chem. Ges.54, 2527–2555 (1921).

    Google Scholar 

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With 19 Figures in the Text, of which 8 in Colour

During various phases of this study technical assistance was given byVivian Burton, Fred Giambront, Peggy Glover, Marie Hitchcock, andSue Lofland. This work was supported byGrant C-3420 (C1,2) from the National Cancer Institute of the U. S. Public Health Service and aided in part by contract no. AT-(40-1)-2432 from the U. S. Atomic Energy Commission.

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Kasten, F.H. Schiff-type reagents in cytochemistry. Histochemie 1, 466–509 (1959). https://doi.org/10.1007/BF00736403

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