Abstract
A high degree of mitotic asynchrony is reported for spontaneously arising multinucleate cells of HeLa and Pt-K1, tissue culture cell lines. Neither nuclear number, nor nuclear size nor the presence or absence of nucleoli, could be directly related to the asynchronous behaviour of the cells. Suboptimal levels of nutrients led to a higher frequency of asynchrony. A partial compartmentation of the cytoplasm could be detected in some of the multinucleate cells. The compartmentation of the cytoplasm may lead to an uneven distribution of inducer and non-inducer metabolites. All these factors together may be responsible for the high degree of mitotic asynchrony.
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References
Das, N.K.: Synthetic capacities of chromosome fragments correlated with their ability to maintain nucleolar material. J. Cell Biol. 15, 121–130 (1962)
Gimenez-Martin, G., De La Torre, C., Lopez-Saez, J.F., Esponda, P.: Plant Nucleolus: Structure and physiology. Cytobiologie 14, 421–462 (1977)
González-Fernández, A., Giménez-Martín, G., Díez, J.L., Torre, C. de la, López-Sáez, J.F.: Interphase development and beginning of mitosis in the different nuclei of polynucleate homokaryotic cells. Chromosoma (Berl.) 36, 100–111 (1971)
Harris, H., Watkins, J.F.: Hybrid cells derived from mouse and man: Artificial heterokaryons of mammalian cells from different species. Nature (Lond.) 205, 640–646 (1965)
Heneen, W.K.: In situ analysis of cultured Potorous cells. I. DNA synthesis and mitotic dynamics in bi- and multinucleate cells. Hereditas (Lund) 67, 221–250 (1971)
Johnson, R.T., Harris, H.: DNA synthesis and mitosis in fused cells. I. HeLa homokaryons. J. Cell Sci. 5, 603–624 (1969)
Johnson, R.T., Rao, P.N.: Nucleocytoplasmic interactions in the achievement of nuclear synchrony in DNA synthesis and mitosis in multinucleate cells. Biol. Rev. 46, 97–155 (1971)
Kato, H., Sandberg, A.A.: Chromosome pulverization in human binucleate cells following colcemid treatment. J. Cell Biol. 34, 35–45 (1967)
Kato, H., Sandberg, A.A.: Chromosome pulverization in human cells with micronuclei. J. nat. Cancer Inst. 40, 165–175 (1968)
Koller, P.C.: The experimental modification of nucleic acid synthesis in the cell. Symp. Soc. exp. Biol. 1, 270–290 (1947)
Krishan, A., Ray-Chaudhury, R.: Asynchrony of nuclear development in cytochalasin-induced multinucleate cell. J. Cell Biol. 43, 618–621 (1969)
Nilova, V.K., Sukhanova, K.M.: Synthesis of nucleic acids in Opalina ranarum Ehrbg. Nature (Lond.) 204, 459–460 (1964)
Oftebro, R., Wolf, I.: Mitosis of bi- and multinucleate HeLa cells. Exp. Cell Res. 48, 39–52 (1967)
Rao, P.N., Johnson, R.T.: Mammalian cell fusion studies on the regulation of DNA synthesis and mitosis. Nature (Lond.) 225, 159–164 (1970)
Stubblefield, E.: DNA synthesis and chromosomal morphology of Chinese hamster cells cultured in media containing N-deacetyl-N-methyl colchicine (colcemid). In: Cytogenetics of cells in culture (R.J.C. Harris, ed.), pp. 223–248. New York: Academic Press 1964
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Ghosh, S., Paweletz, N. & Ghosh, I. Mitotic asynchrony of multinucleate cells in tissue culture. Chromosoma 65, 293–300 (1978). https://doi.org/10.1007/BF00286409
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DOI: https://doi.org/10.1007/BF00286409