Abstract
We have used a combination of chromosome sorting, degenerate oligonucleotide-primed polymerase chain reaction (DOP-PCR), chromosome painting and digital image capturing and processing techniques for comparative chromosome analysis of members of the genus Muntiacus. Chromosome-specific “paints” from a female Indian muntjac were hybridised to the metaphase chromosomes of the Gongshan, Black, and Chinese muntjac by both single and three colour chromosome painting. Karyotypes and idiograms for the Indian, Gongshan, Black and Chinese muntjac were constructed, based on enhanced 4′, 6-diamidino-2-phenylindole (DAPI) banding patterns. The hybridisation signal for each paint was assigned to specific bands or chromosomes for all of the above muntjac species. The interspecific chromosomal homology was demonstrated by the use of both enhanced DAPI banding and comparative chromosome painting. These results provide direct molecular cytogenetic evidence for the tandem fusion theory of the chromosome evolution of muntjac species.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Brinkley BR, Valdivia MM, Tousson A, Brenner SL (1984) Compound kinetochores of the Indian muntjac. Evolution by linear fusion of unit kinetochores. Chromosoma 91:1–111
Carrano AV, Gray JW, Moore II DH, Minkler JL, Mayall BH, Van Dilla MA, Mendelsohn ML (1976) Purification of the chromosomes of the Indian muntjac by flow sorting. J Histochem Cytochem 24:348–354
Carter NP, Ferguson-Smith ME, Affara NA, Briggs H, Ferguson-Smith MA (1990) Study of X chromosome abnormality in XX males using bivariate flow karyotype analysis and flow sorted dot blots. Cytometry 11:202–207
Carter NP, Ferguson-Smith MA, Perryman MT, Telenius H, Pelmear AH, Leversha MA, Glancy MT, Wood SL, Cook K, Dyson HM, Ferguson-Smith ME, Willatt LR (1992) Reverse chromosome painting: a method for the rapid analysis of aberrant chromosomes in clinical cytogenetics. J Med Genet 29:299–307
Cremer T, Lichter P, Boren J, Ward DC, Manulidis L (1988) Detection of chromosome aberrations in metaphase and interphase tumour cells by in situ hybridisation using chromosome-specific library probes. Hum Genet 80:235–236
Dauwerse JG, Wiegant J, Raap AK, Breuning MH, van Ommen GJB (1992) Multiple colors by fluorescence in situ hybridization using ratio-labelled DNA probes create a molecular karyotype. Hum Mol Genet 1:593–598
Elder FFB, Hsu TC (1988) Tandem fusions in the evolution of mammalian chromosomes. In: Daniel A (ed) The cytogenetics of mammalian autosomal rearrangements. Alan R Liss, New York, pp 481–506
Fontana F, Rubini M (1990) Chromosomal evolution in Cervidae. Biosystems 24:157–174
Hsu TC, Pathak S, Chen TR (1975) The possibility of latent centromeres and a proposed nomenclature system for total chromosome and whole arm translocations. Cytogenet Cell Genet 15:41–49
Jauch R, Wienberg J, Stanyon R, Arnold N, Tofanelli S, Ishida T, Cremer T (1992) Reconstruction of genomic rearrangements in great apes and gibbons by chromosome painting. Proc Natl Acad Sci USA 89:8611–8615
Lan H, Shi L (1994) Restriction endonuclease analysis of mitochondrial DNA of muntacs and related deer. Sci Chin [Series BJ 37:294–302
Lee C, Sasi R, Lin CC (1993) Interstitial localization of telomeric DNA sequences in the Indian muntjac chromosomes: further evidence for tandem fusions in the karyotypic evolution of the Asian muntjacs. Cytogenet Cell Genet 63:156–159
Levy HP, Schultz RA, Ordonez JV, Cohen MM (1991) Anti-kinetochore staining for single laser, bivariate flow sorting of Indian muntjac chromosomes. Cytometry 12:695–700
Levy HP, Schultz RA, Ordonez JV, Cohen MM (1993) DNA content measurements and an improved idiogram for the Indian muntjac Cytometry 14:362–368
Lichter P, Cremer T, Borden J, Manuelidis L, Ward DC (1988) Delineation of individual human chromosomes in metaphase and interphase cells by in situ suppression hybridisation using recombinant DNA libraries. Hum Genet 80:224–234
Lin CC, Sasi R, Fan Y-S, Chen Z-Q (1991) New evidence for tandem chromosome fusions in the karyotypic evolution of Asian muntjacs. Chromosoma 101:19–24
Ma K, Shi L (1988) Studies on synaptonemal complexes in spermatocytes of Chinese muntjac, Black muntjac, and Indian muntjac. Chin J Genet 15:283–292
Ma S, Wang Y, Shi L (1990) A new species of the genus Muntiacus from Yunnan China. Zool Res 11:47–53 (in Chinese)
Neitzel H (1987) Chromosome evolution of Cervidae: Karyotype and molecular aspects. In: Obe G, Basler A (eds) Cytogenetics-Basic and applied aspects. Springer, Berlin Heidelberg New York, pp 91–112
Pinkel D, Landegent J, Collins C, Fuscoe J, Seagraves R, Lucas J, Gray JW (1988) Fluorescence in situ hybridisation with human chromosome specific libraries: Detection of trisomy 21 and translocations of chromosomes 4. Proc Natl Acad Sci USA 85:9138–9142
Scherthan H, Cremer T, Arnason U, Weier H-U, Lima-de-Faria A, Fronicke L (1994) Comparative chromosome painting discloses homologous segments in distantly related mammals. Nature Genet 6:342–347
Shi L, Ye Y, Duan X (1980) Comparative cytogenetic studies on the red muntjac, Chinese muntjac and their F1 hybrids. Cytogenet Cell Genet 26:22–27
Shi L (1983) Sex-linked chromosome polymorphism in black muntjac Muntiacus crinifrons In: Swaminathan MS (ed) Proceedings of the fifteenth international congress of genetics, Oxford & IBH Publication, New Delhi
Shi L, Ma C (1988) A new karyotype of muntjac (Muntiacus sp.) from Gongshan county in China. Zool Res 9:343–347 (in Chinese)
Shi L, Pathak S (1981) Gametogenesis in a Indian muntjac X Chinese muntjac hybrid. Cytogenet Cell Genet 30:152–156
Soma H, Kada H, Mtayoshi K, Susuki Y, Meckvichal C, Mahannop A, Vatanaromya B (1983) The chromosomes of Muntiacus feae. Cytogenet Cell Genet 35:156–158
Spathas D, Ferguson-Smith MA (1993) A simplified one step procedure for enhanced detection of biotinylated probes with fluorescein conjugates. Trends Genet 9:262
Telenius H, Pelmear A, Tunnacliffe A, Carter NP, Behmel A, Ferguson-Smith MA, Nordenskjold M, Pfragner R, Ponder B (1992) Cytogenetic analysis by chromosome painting using DOP-PCR amplified flow-sorted chromosomes. Genes Chromosomes Cancer 4:257–263
Wienberg J, Stanyon R, Jauch A, Cremer T (1990) Molecular cytotaxonomy of primates by chromosomal in situ suppression hybridisation. Genomics 8:347–350
Wienberg J, Stanyon R, Jauch A, Cremer T (1992) Homologies in human and Macaca fuscata chromosomes revealed by in situ suppression hybridisation with human chromosome specific DNA libraries. Chromosoma 101:265–270
Wurster DH, Benirschke K (1967) Chromosome studies in some deer, the springbok and the spronghorn, with notes on placentation in deer. Cytologia 32:273–285
Wurster DH, Benirschke K (1970) Indian muntjac, Muntiacus muntjak: a deer with a low diploid chromosome number. Science 168:1363–1366
Author information
Authors and Affiliations
Additional information
Deceased
Rights and permissions
About this article
Cite this article
Yang, F., Carter, N.P., Shi, L. et al. A comparative study of karyotypes of muntjacs by chromosome painting. Chromosoma 103, 642–652 (1995). https://doi.org/10.1007/BF00357691
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00357691