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Molecular characterization of an 18 kb segment of DNA puff C4 of Bradysia hygida (Diptera, Sciaridae)

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Abstract

The data presented here are an extension of the molecular characterization of DNA puff C4 of Bradysia hygida. A cDNA related to a gene amplified in this puff and expressed when puff C4 expands was cloned and sequenced. Analysis of the amino acid sequence deduced from the open reading frame present in the cDNA indicate that the encoded protein is secreted and comprises mostly α-helical coiled-coil. An 18 kb genomic segment containing the transcription unit of this gene was also cloned and the structure and expression of the 1.4 kb mRNA was determined. Quantitative slot blot hybridization of DNA complementary to the transcription unit shows that this gene is amplified about 21 times in the salivary gland, confirming data previously obtained. Fragments upstream of the 5′ end, and beyond the 3′ end, of the gene transcription unit were also analysed and shown to be amplified at least eight and five times, respectively. Based on these data we discuss how amplification could occur at DNA puffs.

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Authors and Affiliations

  1. Departamento de Morfologia, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, 14049-900, Ribeirão Preto, SP, Brazil

    Nadia Monesi, Aparecida M. Fontes, Luiz R. Basso Jr & Maria Luisa Paçó-Larson

  2. Departmento de Biologia, Universidade Estadual de Maringá, PR, Brazil

    M. Aparecida Fernandez

  3. Department of Biology, Faculty of Pharmaceutical Sciences, Kanazawa University, Kanazawa, Japan

    Yoshinobu Nakanishi

  4. Unité de Génétique Somatique (URA CNRS 361), Institut Pasteur, 25 rue du Dr. Roux, F-75724, Paris Cédex 15, France

    Bruno Baroh & Gerard Buttin

Authors
  1. Nadia Monesi
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  2. M. Aparecida Fernandez
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  3. Aparecida M. Fontes
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  4. Luiz R. Basso Jr
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  5. Yoshinobu Nakanishi
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  6. Bruno Baroh
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  7. Gerard Buttin
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  8. Maria Luisa Paçó-Larson
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Monesi, N., Fernandez, M.A., Fontes, A.M. et al. Molecular characterization of an 18 kb segment of DNA puff C4 of Bradysia hygida (Diptera, Sciaridae). Chromosoma 103, 715–724 (1995). https://doi.org/10.1007/BF00344233

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  • DOI: https://doi.org/10.1007/BF00344233

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