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Metabolism of deoxycytidine in isolated perfused organs

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Summary

The metabolism of deoxycytidine-U-14C was studied in isolated perfused normal and regenerating rat liver, in perfused rat intestine and in isolated perfused mouse liver. A technique to perfuse mouse liver is described. Catabolism of dCyd is very slow in organs of the rat whereas it is rapid in those of the mouse. Nevertheless, incorporation into liver DNA does not differ markedly between the species. The quantitative aspects of dCyd metabolism in the isolated organs are discussed.

Zusammenfassung

Der Stoffwechsel von Deoxycytidin-U-14C wurde in isoliert perfundierter normaler und regenerierter Rattenleber, in Rattendarm und in normaler Mäuseleber untersucht. Eine Methode zur Perfusion der Mäuseleber wird beschrieben. Der Abbau von Deoxycytidin ist langsam in Rattenorganen, jedoch rasch in Mäuseleber. Dabei sind zwischen den beiden Tierarten keine großen Unterschiede beim Einbau in Leber-DNA zu verzeichnen. Die quantitativen Aspekte des Deoxycytidinstoffwechsels in isolierten Organen werden diskutiert.

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Abbreviations

dCyd:

deoxyoytidine

dThd:

deoxythymidine

dUrd:

deoxyuridine

dCMP:

deoxyoytidine monophosphate

BAIBA:

ß-aminoisobutyrio acid

References

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Authors and Affiliations

  1. EURATOM and Radiobiology Department, S.C.K./C.E.N., Mol, Belgium

    G. B. Gerber, B. Zicha, J. Deroo, J. P. de Cock & E. Geyer

Authors
  1. G. B. Gerber
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  2. B. Zicha
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  3. J. Deroo
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  4. J. P. de Cock
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  5. E. Geyer
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Additional information

This is publication No. 754 of the Euratom Biology Division, Contract 078-69 BIAC.

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Gerber, G.B., Zicha, B., Deroo, J. et al. Metabolism of deoxycytidine in isolated perfused organs. Biophysik 8, 333–341 (1972). https://doi.org/10.1007/BF01190619

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  • DOI: https://doi.org/10.1007/BF01190619

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