Summary
Cellulases in forest litter and soil occur in both bound and extractable forms. The proportion of total bound endocellulase activity (not extractable) increases during decomposition, whereas the proportion of bound exocellulase activity remains fairly constant. The proportions of bound enzymes differ among litter types with different chemical compositions. The proportion of bound activity is higher in mineral soil than in litter. We also investigated the effects of anion type (NaCl versus Na2SO4), concentration and pH on the extractability of cellulases and protein in two horizons of two forest soils. The extractability of cellulases increases as pH increases from 3.5 to 5.6. Anion type and concentration did not have consistent effects on extractability. However, there was a trend for higher extractability by sulfate than by chloride and with increasing salinity.
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McClaugherty, C.A., Linkins, A.E. Extractability of cellulases in forest litter and soil. Biol Fert Soils 6, 322–327 (1988). https://doi.org/10.1007/BF00261021
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DOI: https://doi.org/10.1007/BF00261021