Summary
Ovaries from 4-day-old female adults of Gryllus bimaculatus produce about 5 ng of free and conjugated ecdysteroids per hour during a 16-h incubation in Grace's medium. During incubation of pieces of the abdominal integument together with the adjacent segmental fat body, a net synthesis of moulting hormones is observed (2.3 ng per hour per animal), similar to that in the ovary. Separate incubations of disunited abdominal epidermis and segmental fat body tissue result in much lower rates of ecdysteroid synthesis. Ecdysteroid synthesis in ovarian homogenates is about one-third of that in intact organs. This reduction is due to a lack of conjugate formation in homogenates. Homogenates of the abdominal integument complex are no longer capable of synthesizing ecdysteroids. For both tissues, a de novo synthesis of ecdysteroids is corroborated by following the in vitro incorporation of [14C]-label from cholesterol and [3H]-label from 2,22,25-trideoxyecdysone (5β-ketodiol), respectively, into free ecdysone. The rate of incorporation into ecdysone is only 0.0014% for cholesterol but 0.48% for 5β-ketodiol. Both tissues represent primary sources of ecdysteroids in female adult crickets.
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Abbreviations
- HPLC:
-
high performance liquid chromatography
- IU:
-
international units
- NP:
-
normal phase
- RIA:
-
radioimmunoassay
- RP:
-
reversed phase
- SEM:
-
standard error of mean
References
Adams TS, Dillwith JW, Blomquist GJ (1984) The role of 20-hydroxyecdysone in housefly sex pheromone biosynthesis. J Insect Physiol 30:287–294
Behrens W, Hoffmann KH (1983) Effect of exogenous ecdysteroids on reproduction in crickets, Gryllus bimaculatus. Int J Invert Reprod 6:149–159
Bulenda D, Stecher A, Freunek M, Hoffmann KH (1986) Ecdysone metabolism in adult crickets, Gryllus bimaculatus. Insect Biochem 16:83–90
Cassier P, Baehr J-C, Carnelle J-P, Porcheron P, Claret J (1980) The integument and ecdysteroids: in vivo and in vitro studies. In: Hoffmann JA (ed) Progress in ecdysone research. Elsevier, Amsterdam pp 235–246
Delachambre J, Besson MT, Quennedey A, Delbecque JP (1984) Relationships between hormones and epidermal cell cycles during the metamorphosis of Tenebrio molitor. In: Hoffmann JA, Porchet M (eds) Biosynthesis metabolism and mode of action of invertebrate hormones. Springer, Berlin, pp 245–254
Delbecque JP, Meister MF, Quennedey A (1986) Conversion of radiolabelled 2,22,25-trideoxyecdysone in Tenebrio pupae. Insect Biochem 16:57–63
Delbecque JP, Connat JL, Lafont R (1988) Polar and apolar metabolites of ecdysteroids during metamorphosis of Tenebrio molitor. J Insect Physiol 34:619–624
Delbecque JP, Weidner K, Hoffmann KH (1990) Alternative sites for ecdysteroid production in insects. Invert Reprod Develop 18:29–42
Espig W, Thiry E, Hoffmann KH (1989) Ecdysteroids during ovarian development and embryogenesis in the cricket, Gryllus bimaculatus (de Geer). Invert Reprod Develop 15:143–154
Gersch M (1978) Die klassische Vorstellung der hormonalen Steuerung der Insektenentwicklung im Lichte neuer experimenteller Tatsachen: Produktion von Häutungshormonen außerhalb der Prothorakaldrüse. Zool Jahrb Abt Allg Zool Physiol Tiere 82:171–184
Goltzené F, Lagueux M, Charlet M, Hoffmann JA (1978) The follicle cell epithelium of maturing ovaries of Locusta migratoria: a new biosynthetic tissue for ecdysone. Hoppe Seyler's Z Physiol Chem 359:1427–1434
Grieneisen ML, Warren JT, Sakurai S, Gilbert LI (1991) A putative route for ecdysteroids: metabolism of cholesterol in vitro by middly disrupted prothoracic glands of Manduca sexta. Insect Biochem 21:41–51
Hagedorn HH (1985) The role of ecdysteroids in reproduction. In: Kerkut GA, Gilbert LI (eds) Comprehensive insect physiology, biochemistry and pharmacology, vol 8. Pergamon Press, Oxford, pp 205–262
Hagedorn HH (1989) The role of hemolymph ecdysteroids in the adult insect. In: Koolman J (ed) Ecdysone-from chemistry to mode of action. Thieme, Stuttgart, pp 279–289
Hoffmann JA, Lagueux M (1985) Endocrine aspects of embryonic development in insects. In: Kerkut GA, Gilbert LI (eds) Comprehensive insect physiology, biochemistry and pharmacology, vol 1. Pergamon Press, Oxford, pp 435–460
Hoffmann JA, Lagueux M, Hetru C, Kappler C, Goltzené F, Lanot R, Thiebold J (1986) Role of ecdysteroids in reproduction of insects: a critical analysis. In: Porchet M, et al. (eds) Advances in invertebrate reproduction 4. Elsevier, Amsterdam, pp 9–21
Hoffmann KH, Behrens W (1982) Free ecdysteroids in adult male crickets, Gryllus bimaculatus. Physiol Entomol 7:269–279
Hoffmann KH, Espig W, Kempa-Tomm S, Wennauer R (1988) Hormones mediate temperature effects on insect reproduction: new aspects on the role of ecdysteroids and juvenile hormone in ovarian and egg development of Gryllus bimaculatus. In: Sehnal F et al. (eds) Endocrinological frontiers in physiological insect ecology. Technical University Press, Wroclaw, pp 227–242
Koolman J (1990) Ecdysteroids. Zool Sci 7:563–580
Lanot R, Roussel J-P, Thiebold JJ (1989) Ecdysteroids and meiotic reinitiation in oocytes of Periplaneta americana (Dictyoptera) and Gryllus bimaculatus (Orthoptera). Invert Reprod Develop 15:69–75
Lanzrein B, Wilhelm R, Buschor J (1981) On the regulation of corpora allata activity in adult females of the ovoviviparous cockroach Nauphoeta cinerea. In: Pratt GE, Brooks GT (eds) Juvenile hormone biochemistry. Elsevier, Amsterdam, pp 147–160
Leubert F, Eibisch H, Kroschwitz H, Scheffel H (1982) Ecdysteroid Biosynthese durch das Ovar von Lithobius fortificatus (L.) (Chilopoda). Zool Jahrb Abt Zool Physiol Tiere 86:465–476
Locb MJ, Woods CW, Brandt EP, Borkovec AB (1982) Larval testes of the tobacco budworm. A new source of insect ecdysteroids. Science 218:896–898
Loeb MJ, Brandt EP, Woods CW, Bell RA (1988) Secretion of ecdysteroid by sheats of testes of the gypsy moth, Lymantria dispar, and its regulation by testis ecdysiotropin. J Exp Zool 248:94–100
Lynn DE, Feldlaufer MF, Lusby WR (1987) Isolation and identification of 20-hydroxyecdysone from a lepidopteran continuous cell line. Arch Insect Biochem Physiol 5:71–79
Meister MF, Dimarcq J-L, Kappler C, Hetru C, Lagueux M, Lanot R, Luu B, Hoffmann JA (1985) Conversion of a radiolabelled ecdysone precursor, 2,22,25-trideoxyecdysone, by embryonic and larval tissues of Locusta migratoria. Mol Cell Endocrinol 41:27–44
Oberlander H, Leach CE, Lanka S, Willis JH (1988) Ecdysteroid action on moth epithelial tissues and cell lines. Arch Insect Biochem Physiol 5:81–89
Porcheron H, Lafon M, Morinière M, Coudouel N (1988) Production of ecdysteroids by isolated cells from larval epidermis of Locusta migratoria. Comp Endocrinol (Life Sci Adv) 7:7–11
Rees HH (1985) Biosynthesis of ecdysone. In: Kerkut GA, Gilbert LI (eds) Comprehensive insect physiology, biochemistry and pharmacology, vol 7. Pergamon Press, Oxford, pp 249–293
Rees HH (1989) Pathways of biosynthesis of ecdysone. In: Koolman J (ed) Ecdysone — from chemistry to mode of action. Thieme, Stuttgart, pp 152–160
Rees HH, Greenwood DR, Dinan LN, Isaac RE, Goodwin TW (1981) Biosynthesis and metabolism of ecdysteroids. In: Sehnal F, et al. (eds) Regulation of insect development and behaviour. Technical University Press, Wroclaw, pp 71–92
Redfern CPF (1989) Ecdysiosynthetic tissues. In: Koolman J (ed) Ecdysone — from chemistry to mode of action. Thieme, Stuttgart, pp 182–187
Richter K (1984) Die Prothorakaldrüse im Imaginalstadium von Periplaneta americana. Zool Jahrb Abt Zool Physiol Tiere 88:433–440
Robert A, Strambi A, Strambi C, Gonella J (1986) Opposite effects of ecdysone and 20-hydroxyecdysone on in vitro uterus motility of a tsetse fly. Life Sci 39:2617–2622
Romer F (1972) Histologie, Histochemie, Polyploidie und Feinstruktur der Oenocyten von Gryllus bimaculatus (Saltatoria). Cytobiologie Z Exp Zellforsch 6:195–213
Rubenstein EC, Lelly TJ, Schwartz MB, Woods CW (1982) In vitro synthesis and secretion of ecdysteroids by Drosophila melanogaster ovaries. J Exp Zool 223:305–308
Ruegg RP, Orchard I, Davey KG (1982) 20-Hydroxyecdysone as a modulator of electrical activity in neurosecretory cells of Rhodnius prolixus. J Insect Physiol 28:243–248
Smith SL, Bollenbacher WE (1985) Ovarian ecdysteroids and their secretion in late-pharate adults of Galleria mellonella. J Insect Physiol 31:419–424
Soltani N, Delachambre J, Delbecque JP (1989) Specific effects of diflubenzuron on ecdysteroid titers during development of Tenebrio molitor. Evidence for a change in hormonal source. Gen Comp Endocrinol 76:350–356
Thiry E, Hoffmann KH (1992) Dynamics of ecdysone and 20-hydroxyecdysone metabolism after injection and ingestion in Gryllus bimaculatus (de Geer). Zool Jahrb Abt Physiol Tiere 96:17–38
Walgraeve HRMA, Verhaert PDEM (1988) Presence and function of ecdysteroids in invertebrates. ISI atlas of science: plants and animals 1:164–172
Ward GB, Kelly TJ, Woods CW, Marks EP (1987) Ecdysteroid production by a continuous insect cell line. Arch Insect Biochem Physiol 5:91–98
Weidner K (1991) Ecdysonsynthese bei adulten Grillenweibchen (Gryllus bimaculatus de Geer). Dissertation, Univ Ulm, FRG
Weidner K, Hoffmann KH (1992) Endocrinology of cricket reproduction and embryogenesis: environmental aspects. Zool Jahrb Abt Zool Physiol Tiere 96:199–210
Weidner K, Claß M, Rieck H, Hoffmann KH (1992) Developmental changes in ecdysteroid biosynthesis in vitro during adult life cycle and embryogenesis in a cricket, Gryllus bimaculatus (de Geer). Invert Reprod Develop 21:129–140
Wennauer R, Kassel L, Hoffmann KH (1992) The effects of juvenile hormone, 20-hydroxyecdysone, precocene II, and ovariectomy on the activity of the corpora allata (in vitro) in adult female Gryllus bimaculatus. J Insect Physiol 35:299–304
Zhu XX, Gfeller H, Lanzrein B (1983) Ecdysteroids during oögenesis in the ovoviviparous cockroach Nauphoeta cinerea. J Insect Physiol 29:225–235
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Hoffmann, K.H., Weidner, K. & Seidel, M. Sites of ecdysteroid biosynthesis in female adults of Gryllus bimaculatus (Ensifera, Gryllidae). J Comp Physiol B 162, 731–739 (1992). https://doi.org/10.1007/BF00301623
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DOI: https://doi.org/10.1007/BF00301623