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Effects of ion substitution on the transepithelial potential difference of the gills of the fiddler crabUca tangeri: evidence for a H+-pump in the apical membrane

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Summary

  1. 1.

    Transepithelial potential differences (PDte) and acidification rates of the bath chamber were measured on isolated perfused posterior gills of the fiddler crabUca tangeri adapted to dilute seawater.

  2. 2.

    The PDte decreased to almost zero when Na+ was substituted by choline or when ouabain was added to the perfusion saline in high concentrations (10 mmol·l−1). Thus, the rheogenic NaCl-transport across the gill epithelium seems to be totally Na+-dependent.

  3. 3.

    When Cl− was replaced by gluconate, a bath positive PDte occurred which was insensitive to ouabain. This PDte could also be observed when, in addition to Cl− removal, Na+ was replaced by TMA+.

  4. 4.

    Bath acidification under normal conditions could be abolished by ouabain, indicating that there is H+ excretion via electrically silent Na+/H+ exchange. In contrast, bath acidification under Cl−-free conditions is only partially blocked by ouabain.

  5. 5.

    It is concluded that under Cl−-free conditions a rheogenic H+-pump in the apical membrane is responsible for the ouabain-insensitive bath acidification as well as for the PDte.

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Abbreviations

PD te :

transepithelial potential difference

R te :

transepithelial resistance

R in :

input resistance

TMA + :

tetramethylammonium

NEM :

N′-ethylmaleimide

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Authors and Affiliations

  1. I. Physiologisches Institut der Universität des Saarlandes, D-6650, Homburg/Saar, Federal Republic of Germany

    Peter Krippeit-Drews & Gisela Drews

  2. Institut für Tierphysiologie, Freie Universität Berlin, Grunewaldstrasse 34, D-1000, Berlin 41

    Kai Graszynski

Authors
  1. Peter Krippeit-Drews
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  2. Gisela Drews
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  3. Kai Graszynski
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Parts of this study had been published earlier in abstract form

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Krippeit-Drews, P., Drews, G. & Graszynski, K. Effects of ion substitution on the transepithelial potential difference of the gills of the fiddler crabUca tangeri: evidence for a H+-pump in the apical membrane. J Comp Physiol B 159, 43–49 (1989). https://doi.org/10.1007/BF00692682

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  • DOI: https://doi.org/10.1007/BF00692682

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