Abstract
Transformed roots of Catharanthus roseus were obtained following infection of detached leaves with Agrobacterium rhizogenes. Roots would not grow in full strength Gamborg's B5 medium but would grow satisfactorily if the medium was diluted to one half strength. Little alkaloid appeared in the growth medium but root tissue contained a high level and wide variety of alkaloids. Ajmalicine, serpentine, vindolinine and catharanthine were prominent components. Vinblastine could also be detected by a combination of HPLC and radioimmunoassay, though at a level of only 0.05μg/g dry weight.
Similar content being viewed by others
Abbreviations
- B5:
-
Gamborg's B5 nutrient salts
- LC/MS:
-
combined liquid chromatography/mass spectrometry
- FW:
-
fresh weight
- Kb:
-
kilobase
References
Aird ELH, Hamill JD, Rhodes MJC (1988) Plant Cell, Tissue and Organ Culture. In press.
Arens H, Stöckigt J, Weiler EW, Zenk MH (1978) Planta Medica 34: 37–46.
Creasey WA (1984) In Saxton JE (ed) The Chemistry of Heterocyclic Compounds: Indoles; Part Four — The monoterpenoid indole alkaloids. John Wiley and Sons, New York, pp 783–829.
Deus B, Zenk MH (1982) Biotechnol. Bioeng. 24: 1965–1974.
Deus-Neumann B, Zenk MH (1984) Planta Medica 50: 427–431.
Eilert U, Constabel F, Kurz WGW (1986) J. Plant Physiol. 126: 11–22.
Endo T, Goodbody A, Misawa M (1987) Planta Medica 53: 479–482.
Fahn W, Gundlach H, Deus-Neumann B, Stöckigt J (1985) Plant Cell Rep. 4: 333–336.
Feinberg AP, Vogelstein B (1984) Anal. Biochem. 134: 266–267.
Flores HE (1986) In Lebaron H, Mumma RO, Honeycutt RC, Duesing JH (eds) Applications of Biotechnology to Agricultural Chemistry. Am. Chem. Soc. Symp. Series.
Gamborg OL, Miller RA, Ojima K (1968) Exp. Cell Res. 50: 151–158.
Hamill JD, Parr AJ, Robins RJ, Rhodes MJC (1986) Plant Cell Rep. 5: 111–114.
Hamill JD, Parr AJ, Rhodes MJC, Robins RJ, Walton NJ (1987) Bio/Technology 5: 800–804.
Janot M-M, Le Men J, Fan C (1959) Bull. Soc. Chim. France 1959, 891–892.
Jouanin L (1984) Plasmid 12: 91–102.
Knobloch K-H, Berlin J (1980) Z. Naturforsch. 35C: 551–556.
Kurz WGW, Chatson KB, Constabel F, Kutney JP, Choi LSL, Kolodziejczyk P, Sleigh SK, Stuart KL, Worth BR (1980) Phytochemistry 19: 2583–2587.
Miura Y, Hirata K, Kurano N (1987) Agric. Biol. Chem. 51: 611–614.
Murashige T, Skoog F (1962) Physiol. Plant. 15: 473–497.
Parr AJ, Hamill JD (1987) Phytochemistry 26: 3241–3245.
Payne J, Hamill JD, Robins RJ, Rhodes MJC (1987) Planta Medica 53: 474–478.
Renaudin J-P (1984) J. Chromatogr. 291: 165–174.
Smith JI, Smart NJ, Kurz WGW, Misawa M, (1987) J. Exp. Bot. 38: 1501–1506.
Svoboda GH, Blake DA (1975) In “The Catharanthus Alkaloids”. Eds Taylor WI, Farnsworth NR, Marcel Dekker, New York, pp 45–83.
Svoboda GH, Oliver AT, Bedwell DR (1963) Lloydia 26: 141–153.
Teale JD, Clough JM, Marks V (1977) Br. J. Clin. Pharmac. 4: 169–172.
Verpoorte R, Baerheim Svendsen A (1983) Chromatography of Alkaloids. Part A, Thin-layer Chromatography. Elsevier, Amsterdam (J. Chromatogr. Library volume 23A).
White PR (1954) The Cultivation of Animal and Plant Cells. The Ronald Press, New York.
Author information
Authors and Affiliations
Additional information
Communicated by W. Barz
Rights and permissions
About this article
Cite this article
Parr, A.J., Peerless, A.C.J., Hamill, J.D. et al. Alkaloid production by transformed root cultures of Catharanthus roseus . Plant Cell Reports 7, 309–312 (1988). https://doi.org/10.1007/BF00269925
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00269925