Abstract
Callus was induced on the wounded immature seeds and mature zygotic embryos of Dysosma pleiantha (Hance) Woodson (Berberidaceae) on a medium based on Murashige and Skoog's (1962) formula supplemented with 1 mg/l 2,4-dichlorophenoxy-acetic acid (2,4-D). Spontaneous embryoid formation occurred on the media containing low concentrations of 2,4-D (0.1–0.5 mg/l). These embryoids germinated in either MS or B5 medium containing 1 mg/l N6-benzyladenine and 1 mg/l gibberellic acid. The regenerated plantlets were successfully transferred to soil.
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Abbreviations
- 2,4-D:
-
2,4-dichlorophenoxyacetic acid
- BA:
-
N6-benzyladenine
- GA3 :
-
gibberellic acid
- MS medium:
-
Murashige and Skoog's (1962) medium
References
Chuang MJ, Chang WC (1987) J Plant Physiol 128:279–283
Gamborg OL, Miller RA, Ojima L (1968) Exp Cell Res 50:151–158
Hartwell J, Schrecker AW (1958) Forscher Chem Org Naturst 15:83–166
Lee MH, Chen FC (1984) Scientific Taiwan 38:49–74
Murashige T, Skoog F (1962) Physiol Plant 15:473–497
Shibata S, Murata T, Fujita M (1962) Yakugaku Zasshi 82:777–779
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Communicated by J.M. Widholm
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Chuang, Mj., Chang, Wc. Somatic embryogenesis and plant regeneration in callus culture derived from immature seeds and mature zygotic embryos of Dysosma pleiantha (Hance) Woodson. Plant Cell Reports 6, 484–485 (1987). https://doi.org/10.1007/BF00272789
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DOI: https://doi.org/10.1007/BF00272789