Skip to main content
SpringerLink
Log in

A new bioassay system for screening high berberine-producing cell colonies of Thalictrum minus

  • Published:
Plant Cell Reports Aims and scope Submit manuscript

Abstract

A new method of estimating the amount of berberine released from minute cell colonies of Thalictrum minus has been devised to facilitate the selection of high berberine-producing cell lines. In this system, cell aggregates obtained from a cell suspension culture are grown on small pieces of an agar culture medium and the concentration of berberine which has been released from the cells into the agar piece is assayed by the antibacterial activity against Bacillus cereus MT2026. Screening of 1000 cell colonies by the “agar piece method” has resulted in the isolation of four, high berberine-producing cell lines, although they have been found to be more or less unstable with respect to the biosynthetic capability during successive subcultures.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Ikuta A, Syono K, Furuya T (1975) Phytochemistry 14: 1209–1210

    Google Scholar 

  • Ikuta A, Itokawa H (1982) Phytochemistry 21: 1419–1421

    Google Scholar 

  • Nakagawa K, Konagai A, Fukui H, Tabata M (1984) Plant Cell Reports 3: 254–257

    Google Scholar 

  • Yamada Y, Sato F (1981) Phytochemistry 20: 545–547

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Bioscience Research Center, Mitsui Petrochemical Industries, Ltd, Waki-cho, Kuga-gun, 740, Yamaguchi-ken, Japan

    Toshiyuki Suzuki, Toshihiro Yoshioka, Yasuhiro Hara & Yasuhiro Fujita

  2. Faculty of Pharmaceutical Sciences, Kyoto University, Sakyo-ku, 606, Kyoto, Japan

    Mamoru Tabata

Authors
  1. Toshiyuki Suzuki
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Toshihiro Yoshioka
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Yasuhiro Hara
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Mamoru Tabata
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Yasuhiro Fujita
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Communicated by F. Constabel

Rights and permissions

Reprints and permissions

About this article

Cite this article

Suzuki, T., Yoshioka, T., Hara, Y. et al. A new bioassay system for screening high berberine-producing cell colonies of Thalictrum minus . Plant Cell Reports 6, 194–196 (1987). https://doi.org/10.1007/BF00268477

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00268477

Keywords

Search

Navigation